Melatonin directly stimulates the secretion of progesterone by human and bovine granulosa cells in vitro

Webley, G. E.; Lück, Martin

doi:10.1530/jrf.0.0780711

Cited by 103 publications

(48 citation statements)

References 17 publications

(21 reference statements)

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“…A limited number of earlier studies had brought controversial results. Thus, no consistent effects of melatonin on aromatase activity were described in human, porcine or bovine granulose cells cultured in a serum-supplemented medium (Webley and Luck, 1986;Tanavde and Maitra, 2003). Other authors attributed the low sperm quality of human seminal plasma to a low aromatase activity dependent on high endogenous melatonin levels (Yie et al, 1991) or long-term melatonin administration (Luboshitzky et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

Inhibitory effects of pharmacological doses of melatonin on aromatase activity and expression in rat glioma cells

et al. 2007

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Melatonin exerts oncostatic effects on different kinds of neoplasias, especially on oestrogen-dependent tumours. Recently, it has been described that melatonin, on the basis of its antioxidant properties, inhibits the growth of glioma cells. Glioma cells express oestrogen receptors and have the ability to synthesise oestrogens from androgens. In the present study, we demonstrate that pharmacological concentrations of melatonin decreases the growth of C6 glioma cells and reduces the local biosynthesis of oestrogens, through the inhibition of aromatase, the enzyme that catalyses the conversion of androgens into oestrogens. These results are supported by three types of evidence. Firstly, melatonin counteracts the growth stimulatory effects of testosterone on glioma cells, which is dependent on the local synthesis of oestrogens from testosterone. Secondly, we found that melatonin reduces the aromatase activity of C6 cells, measured by the tritiated water release assay. Finally, by (RT) -PCR, we found that melatonin downregulates aromatase mRNA steady-state levels in these glioma cells. We conclude that melatonin inhibits the local production of oestrogens decreasing aromatase activity and expression. By analogy to the implications of aromatase in other forms of oestrogen-sensitive tumours, it is conceivable that the modulation of the aromatase by pharmacological melatonin may play a role in the growth of glioblastomas.

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Section: Discussionmentioning

confidence: 99%

Inhibitory effects of pharmacological doses of melatonin on aromatase activity and expression in rat glioma cells

et al. 2007

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“…Melatonin-binding sites have been detected in granulosa-luteal cells in humans (Yie et al 1995, Woo et al 2001) and in cattle (Wang et al 2012). Previous studies demonstrated that melatonin significantly increased P 4 production in in vitro-cultured human and bovine granulosa cells (Webley & Luck 1986). Similarly, this effect has been described in many other species, including rats and sheep (Durotoye et al 1997, Adriaens et al 2006.…”

Section: Discussionmentioning

confidence: 75%

Melatonin deprival modifies follicular and corpus luteal growth dynamics in a sheep model

Manca¹,

Manunta²,

Spezzigu³

et al. 2014

Reproduction

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This study assessed the effect of melatonin deprival on ovarian status and function in sheep. Experimental procedures were carried out within two consecutive breeding seasons. Animals were divided into two groups: pinealectomised (nZ6) and sham-operated (nZ6). The completeness of the pineal gland removal was confirmed by the plasma concentration of melatonin. Ovarian status was monitored by ovarian ultrasonography for 1 year to study reproductive seasonality. Follicular and corpus luteal growth dynamics were assessed during an induced oestrous cycle. As the effects of melatonin on the ovary may also be mediated by its antioxidant properties, plasma Trolox equivalent antioxidant capacity (TEAC) was determined monthly for 1 year. Pinealectomy significantly extended the breeding season (310G24.7 vs 217.5G24.7 days in controls; P!0.05). Both pinealectomised and sham-operated ewes showed a well-defined wave-like pattern of follicle dynamics; however, melatonin deficiency caused fewer waves during the oestrous cycle (4.3G0.2 vs 5.2G0.2; P!0.05), because waves were 1 day longer when compared with the controls (7.2G0.3 vs 6.1G0.3; P!0.05). The mean area of the corpora lutea (105.4G5.9 vs 65.4G5.9 mm 2 ; P!0.05) and plasma progesterone levels (7.1G0.7 vs 4.9G0.6 ng/ml; P!0.05) were significantly higher in sham-operated ewes compared with pinealectomised ewes. In addition, TEAC values were significantly lower in pinealectomised ewes compared with control ones. These data suggest that melatonin, besides exerting its well-known role in the synchronisation of seasonal reproductive fluctuations, influences the growth pattern of the follicles and the steroidogenic capacity of the corpus luteum. Free Italian abstractAn Italian translation of this abstract is freely available at

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“…The involvement of melatonin in the steroid genesis of human ovaries has been documented (19,20). Webley and Luck (19) also reported direct stimulation of melatonin on hCG.…”

Section: Discussionmentioning

confidence: 97%

The influences of weather on patients with different ovarian responses in the treatment of assisted reproductive technology

Chang

Lan

Chen

et al. 2005

J Assist Reprod Genet

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Purpose : The purpose of this study was to evaluate the influences of tropical weather on patients with different ovarian responses in the treatment of assisted reproductive technology. Methods: Six-hundred fourty-seven women underwent their first treatment cycles were retrospectively analyzed. Patients received embryo transfer either 3 days or 5 days after oocyte retrieval, depending on the number and quality of embryos on day-2.Results : Significant correlations were demonstrated in the top quality embryo rates of day-3 and day-5 embryo transfers with temperature, humidity, and atmosphere pressure. The cumulative light hours negatively correlated with the implantation and pregnancy rates of day-3 embryo transfer (−.282 and −.282, respectively), while they positively correlated with those of day-5 embryo transfer (.225 and .224, respectively). Conclusions : These results clearly suggest that weather may exert influences on the outcome of assisted reproductive technology. Patients with different ovarian responses or blastocyst culture and transfer may modify weather influences.

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Melatonin directly stimulates the secretion of progesterone by human and bovine granulosa cells in vitro

Cited by 103 publications

References 17 publications

Inhibitory effects of pharmacological doses of melatonin on aromatase activity and expression in rat glioma cells

Inhibitory effects of pharmacological doses of melatonin on aromatase activity and expression in rat glioma cells

Melatonin deprival modifies follicular and corpus luteal growth dynamics in a sheep model

The influences of weather on patients with different ovarian responses in the treatment of assisted reproductive technology

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