Melatonin antagonizes ABA action to promote seed germination by regulating Ca2+ efflux and H2O2 accumulation

Li, Hao; Guo, Yanliang; Lan, Zhixiang; Zhang, Zixing; Ahammed, Golam Jalal; Chang, Jianmei; Zhang, Yong; Wei, Chunhua; Zhang, Xian

doi:10.1016/j.plantsci.2020.110761

Cited by 49 publications

(36 citation statements)

References 60 publications

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“…Consistently, our results showed that melatonin alleviated chilling-induced H 2 O 2 accumulation and lipid peroxidation after watermelon seedlings were exposed to 4 °C for 48 h ( Figure 1 ). Many studies have shown that H 2 O 2 plays an essential signaling role in melatonin-mediated regulation of various physiological processes, including stomatal closure, seed germination, lateral root formation, and response to environmental stimulus [ 19 , 29 , 60 , 61 ]. A recent report revealed that H 2 O 2 mediates melatonin-induced cold tolerance in grafted watermelon plants [ 24 ].…”

Section: Discussionmentioning

confidence: 99%

“…Various Ca 2+ sensors decode the Ca 2+ signal evoked by Ca 2+ transient changes, subsequently regulating the expression of CBFs and cold responsive ( COR ) genes [ 8 , 65 ]. Several studies have confirmed that Ca 2+ signaling is involved in melatonin-induced stomatal closure, seed germination, and salt tolerance in plants [ 19 , 29 , 66 ]. However, the relationship between Ca 2+ and melatonin in response to cold stress remains unclear.…”

Section: Discussionmentioning

confidence: 99%

“…The gene-specific primers used for the qRT-PCR are listed in Table S1 . The qRT-PCR amplification was conducted under the conditions reported by Li et al [ 29 ]. β-actin or AtActin2 served as the internal control genes for the normalization of gene expression [ 11 , 44 ].…”

Section: Methodsmentioning

confidence: 99%

“…The seedlings were cultivated in a growth chamber under photoperiod of 12/12 h (day/night), temperature of 28/18 • C (day/night), and a photosynthetic photo flux density (PPFD) of 600 µmol m −2 s −1 . Arabidopsis seeds of Atrbohd (SALK_120299) mutant with Columbia-0 (Col-0) genetic background were received from the Arabidopsis Biological Resource Center (https://www.arabidopsis.org/, accessed date 16 May 2019) [29]. After surface sterilization with 75% ethanol and 3% sodium hypochlorite, Arabidopsis seeds were sown on half-strength Murashige-Skoog (MS) medium containing 0.8% agar and 1.0% sucrose and cultured at 22 • C in a growth chamber under 16/8 h photoperiod.…”

Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

“…Increasing evidence indicates that H 2 O 2 and Ca 2+ signaling facilitate melatonin-mediated regulation of multiple physiological processes, including seed germination and stomatal closure [ 19 , 29 ]. These findings raise a presumption that H 2 O 2 and Ca 2+ signaling may also have a critical role in the melatonin-induced CBF-responsive pathway and subsequent chilling tolerance.…”

Section: Introductionmentioning

confidence: 99%

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Positive Interaction between H2O2 and Ca2+ Mediates Melatonin-Induced CBF Pathway and Cold Tolerance in Watermelon (Citrullus lanatus L.)

Chang

Guo

et al. 2021

Antioxidants

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Cold stress is a major environmental factor that detrimentally affects plant growth and development. Melatonin has been shown to confer plant tolerance to cold stress through activating the C-REPEAT BINDING FACTOR (CBF) pathway; however, the underlying modes that enable this function remain obscure. In this study, we investigated the role of H2O2 and Ca2+ signaling in the melatonin-induced CBF pathway and cold tolerance in watermelon (Citrullus lanatus L.) through pharmacological, physiological, and genetic approaches. According to the results, melatonin induced H2O2 accumulation, which was associated with the upregulation of respiratory burst oxidase homolog D (ClRBOHD) during the early response to cold stress in watermelon. Besides, melatonin and H2O2 induced the accumulation of cytoplasmic free Ca2+ ([Ca2+]cyt) in response to cold. This was associated with the upregulation of cyclic nucleotide-gated ion channel 2 (ClCNGC2) in watermelon. However, blocking of Ca2+ influx channels abolished melatonin- or H2O2-induced CBF pathway and cold tolerance. Ca2+ also induced ClRBOHD expression and H2O2 accumulation in early response to cold stress in watermelon. Inhibition of H2O2 production in watermelon by RBOH inhibitor or in Arabidopsis by AtRBOHD knockout compromised melatonin-induced [Ca2+]cyt accumulation and melatonin- or Ca2+-induced CBF pathway and cold tolerance. Overall, these findings indicate that melatonin induces RBOHD-dependent H2O2 generation in early response to cold stress. Increased H2O2 promotes [Ca2+]cyt accumulation, which in turn induces H2O2 accumulation via RBOHD, forming a reciprocal positive-regulatory loop that mediates melatonin-induced CBF pathway and subsequent cold tolerance.

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