Melatonin and vitamin E limit nitric oxide-induced lipid peroxidation in rat brain homogenates

Escames, Germaine; Guerrero, Juan M.; Reíter, Russel J.; García, Joaquín J.; Muñóz-Hoyos, Antonio; Ortíz, Genaro Gabriel; Oh, Chang Seok

doi:10.1016/s0304-3940(97)00498-9

Cited by 93 publications

(57 citation statements)

References 24 publications

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“…The erythrocyte α-tocopherol level is a sensitive and early index of oxidative stress [20, 21]. The molecule is the major chain-breaking antioxidant scavenging peroxide radicals and protecting membranes against ROS- and NO-induced peroxidative reactions [22, 23]. The increased generation of ROS and NO by monocytes and erythrocytes in the peripheral blood has been demonstrated previously, though those studies were mainly carried out during the active phase of the disease [2, 4, 24].…”

Section: Discussionmentioning

confidence: 99%

Alpha-Tocopherol and NADPH in the Erythrocytes and Plasma of Multiple Sclerosis Patients

et al. 2003

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Objectives: To investigate the influence of interferon-β-1b (INF-β-1b) therapy on blood antioxidants (α-tocopherol and NADPH) in multiple sclerosis (MS). Patients and Methods: Patients with relapsing-remitting MS (n = 14) have been studied during 6 months of INF-β-1b therapy. α-Tocopherol was determined by HPLC and UV or electrochemical detection; NADPH was quantified spectrophotometrically. Results: The erythrocyte α-tocopherol level was reduced (p < 0.001) before treatment, but had regained the control level by 6 months of therapy. The plasma α-tocopherol/lipid ratios were constant during therapy. Plasma triglyceride levels were transiently increased (p = 0.0270) after 1 month of treatment. INF-β-1b had also induced a transient decrease in NADPH after 1 month, but thereafter the level returned to approximately the initial value (p = 0.0248). Conclusion: INF-β-1b seems to exert a sparing effect toward the erythrocyte α-tocopherol content. The fall in NADPH in parallel to the rise in plasma triglycerides suggests stimulation of fatty acid synthesis by INF-β-1b.

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Section: Discussionmentioning

confidence: 99%

Alpha-Tocopherol and NADPH in the Erythrocytes and Plasma of Multiple Sclerosis Patients

et al. 2003

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“…MDA and 4-HNE, the final products of LPO, were measured by a colorimetric assay using the Bioxytech LPO-586 colorimetric kit assay (OXIS Res, Portland, OR, USA) [19]. Briefly, tissue samples were homogenized in 20 volumes (w/v) of ice-cold 20 mM Tris-HCl buffer, pH 7.4.…”

Section: Lipid Peroxidation Assaymentioning

confidence: 99%

Synergistic effects of melatonin and deprenyl against MPTP-induced mitochondrial damage and DA depletion

Khaldy

Escames

León

et al. 2003

Neurobiology of Aging

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Previous studies showed a synergistic effect of melatonin and deprenyl against dopamine (DA) autoxidation in vitro. Since oxidative stress is implicated in Parkinson's disease (PD), we explored the effects of melatonin plus deprenyl administration in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced PD in C57/Bl6 mice. Melatonin, but not deprenyl prevents the inhibition of mitochondrial complex I and the oxidative damage in nigrostriatal neurons induced by MPTP. With the dose used deprenyl recovers 50% DA levels and tyrosine hydroxylase activity depressed by the neurotoxin, normalizing locomotor activity of mice. Melatonin, which was unable to counteract MPTP-induced DA depletion and inhibition of tyrosine hydroxylase activity, potentiates the effect of deprenyl on catecholamine turnover and mice ambulatory activity. These results suggest a dissociation of complex I inhibition from DA depletion in this model of Parkinson's disease. The data also support that a combination of melatonin, which improves mitochondrial electron transport chain and reduces oxidative damage, and deprenyl, which promotes the specific function of the rescued neurons, i.e. DA turnover, may be a promising strategy for the treatment of PD.

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“…Other studies have identified melatonin as an antistress [12, 13], anticonvulsant [14, 15]and antiexcitotoxic [16, 17, 18]compound. The antioxidant properties of melatonin [19, 20, 21]are now recognized to be the basis for some of its effects, including its antistress property.…”

Section: Introductionmentioning

confidence: 99%

Melatonin and β-Endorphin Changes in Children Sensitized to Olive and Grass Pollen after Treatment with Specific Immunotherapy

Girón-Caro

Muñóz-Hoyos²,

Ruiz-Cosano³

et al. 2001

Int Arch Allergy Immunol

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Background: Specific immunotherapy for respiratory allergy, a seasonal disease, significantly reduces the inflammatory process, attenuating the clinical symptoms. The mechanism for the clinical beneficial effect of immunotherapy has not yet been clarified. Melatonin shows a circadian and seasonal variation and together with the endogenous opioid system plays an immunomodulatory role acting on both specific and nonspecific immunity responses. Thus, the possibility that immunotherapy involved changes in the melatonin-opioid system was investigated. Methods: Thirty-five children aged 3–15 years with rhinitis and asthma due to olive + grass pollen sensitization were studied. The patients were treated with depot extracts containing the identified allergens with increasing doses from 1 to 1,000 IU/ml during 3 months. Melatonin, β-endorphin, total and specific IgE and IgG4 were determined before and after treatment. Results: All children showed a significant improvement of their symptoms at the end of the treatment, coinciding with a significant drop of both melatonin and β-endorphin levels. Total IgE decreased in most of the cases although the mean values did not show significant changes. Specific IgE and IgG4 were also unchanged. A significant correlation between melatonin and β-endorphin and between β-endorphin and IgG4 was found before immunotherapy, and these correlations disappeared when the treatment was finished. Conclusions: The decrease in the levels of melatonin and β-endorphin suggests the disappearance of their stimulating influence on the immune system. Thus, hyposensitization after immunotherapy may involve, at least in part, the inhibition of the immunoenhancing properties of the melatonin-opioid system.

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Melatonin and vitamin E limit nitric oxide-induced lipid peroxidation in rat brain homogenates

Cited by 93 publications

References 24 publications

Alpha-Tocopherol and NADPH in the Erythrocytes and Plasma of Multiple Sclerosis Patients

Alpha-Tocopherol and NADPH in the Erythrocytes and Plasma of Multiple Sclerosis Patients

Synergistic effects of melatonin and deprenyl against MPTP-induced mitochondrial damage and DA depletion

Melatonin and β-Endorphin Changes in Children Sensitized to Olive and Grass Pollen after Treatment with Specific Immunotherapy

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