Melatonin and vitamin C exacerbate <i>Cannabis sativa</i>-induced testicular damage when administered separately but ameliorate it when combined in rats

Alagbonsi, Isiaka Abdullateef; Olayaki, Luqman Aribidesi; Salman, Toyin Mohammed

doi:10.1515/jbcpp-2015-0061

Cited by 29 publications

(32 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cannabis sativa (marijuana), the most widely abused recreational drug, has been reported to have a spermato-toxic effect. Alagbonsi and his colleagues [22,23] observed that Cannabis sativa significantly reduced sperm motility and increased the number of abnormal sperm via oxidative-stress. These authors also demonstrated the effect of Δ 9 -tetrahydrocannabinol, an active compound in Cannabis sativa, on sperm kinetics.…”

Section: Introductionmentioning

confidence: 99%

Codeine-induced sperm DNA damage is mediated predominantly by oxidative stress rather than apoptosis

Ajayi

Akhigbe

2020

Redox Report

View full text Add to dashboard Cite

Background: Opioids have been implicated to induce infertility. Although codeine remains the most used opioid for recreational purpose, no study has documented its effect on sperm quality. Elucidating the effect of codeine on sperm cells and the associated mechanisms may provide an insight into preventing drug-induced sperm damage. Twenty-one New Zealand white rabbits were randomized into three groups; control and codeine-treated. The codeine-treated groups received either 4 or 10mg/kg b.w of codeine for six weeks. Results: Codeine treatment led to significant decrease in sperm count, motility, viability, normal morphology, and sperm membrane integrity. This was associated with significant rise in sperm DNA fragmentation, oxidative damage, and caspase 3 activity. The percentage of sperm DNA fragmentation correlates positively with 8-hydroxy-2'-deoxyguanosine, a biomarker of oxidative DNA damage, and caspase 3 activity, a biomarker of apoptosis. The observed correlation was stronger between sperm DNA fragmentation and oxidative DNA damage than sperm DNA fragmentation and caspase 3 activity. Conclusion: This study revealed that chronic codeine exposure causes sperm DNA fragmentation and poor sperm quality primarily via oxidative stress rather than activation of caspase 3-dependent apoptosis. Findings of the present study may explain drug-induced male factor infertility, particularly, those associated with opioid use.

show abstract

Section: Introductionmentioning

confidence: 99%

Codeine-induced sperm DNA damage is mediated predominantly by oxidative stress rather than apoptosis

Ajayi

Akhigbe

2020

Redox Report

View full text Add to dashboard Cite

show abstract

“…Before 1980, the effect of exogenous chemicals on testes was the key point; after 1980, the adverse effect of xenobiotics on glucose metabolism was studied in testicular cells [59,68]. In 1989, the research focus had shifted to enzymes related to testicular cell glucose metabolism, such as LDH and 6-phosphate dehydrogenase.…”

Section: Discussionmentioning

confidence: 99%

Research Trends and Hotspots Analysis Related to the Effects of Xenobiotics on Glucose Metabolism in Male Testes

Fan

et al. 2018

IJERPH

View full text Add to dashboard Cite

This study aimed to integrate and analyze the existing studies and to explore research trends and hotspots related to the effects of xenobiotics on glucose metabolism in male testes. All articles were retrieved from the PubMed database, from an inception date up to 10 June 2017. CiteSpace software (version 5.1.R8 SE) was used for the co-word cluster analysis. A total of 165 eligible publications were included in this study. In 1949–1959, only two articles were published. After 1960, the number of articles increased steadily. These articles were published in 97 journals, in particular, in the Indian Journal of Experimental Biology (11 articles, 6.7%). Most of the authors (87.0%) only published one article. Only a few established research teams, mostly from the USA, worked consistently in this field. The main xenobiotics that had been studied were medicine and common environmental pollutants, e.g., gossypol, cadmium, di-n-butyl phthalate, and alpha-chlorohydrin. The hotspot keywords were Sertoli cell, lactate dehydrogenase, 6-phosphate dehydrogenase, oxidative stress, and glucose metabolism. The focus of research had been changed overtime. This is the first bibliometric study between xenobiotics and glucose metabolism in the male testes. The findings suggest that environmental pollutants have become a huge concern, and related research should be strengthened.

show abstract

“…We have previously observed in our in-vivo studies that vitamin C exacerbates the CS-induced gonadotoxicity, oxidative stress and reproductive hormonal toxicities, but these CS-induced toxicities were ameliorated only when vitamin C was co-administered with melatonin [7,9,10]. In our very recent in-vitro study, we however observed that melatonin alone ameliorated THC-induced spermatotoxicity by attenuating the THC-induced reduction of hyper-activated motility (HAM) of capacitated sperms [17].…”

Section: Introductionmentioning

confidence: 93%

“…However, its detrimental effects on the male reproductive functions have been well-established. For instance, it decreases germ cell proliferation, reproductive organ weight [6,7], and semen parameters [7][8][9] by eliciting endocrine disruption, hyperprolactinaemia [10], down-regulation of hypothalamic-pituitary-gonadal axis [10,11], and oxidative stress [9]. Delta-9-tetrahydrocannabinol (THC, an active phychoactive compound in CS), prescribed under the name Dronabinol (synthetic THC in sesame oil), is clinically being used for the treatment of acquired immunode ciency syndrome (AIDS)-associated cachexia, multiple sclerosis, and cancer chemotherapyassociated nausea [12,13].…”

Section: Introductionmentioning

confidence: 99%

Vitamin C Ameliorates Tetrahydrocannabinol-Induced Spermatotoxicity In-Vitro

Alagbonsi

Olayaki

2020

Preprint

View full text Add to dashboard Cite

Background: We investigated the in-vitro effects of vitamin C on delta-9-tetrahydrocannabinol (THC)-induced reduction in sperm motility and kinematics. Methods: Rats semen were randomly divided into 5 groups. Groups I-III received placebo, THC (1 mM), and vitamin C (5 mM) respectively. Groups IV and V received THC and vitamin C, but group V was additionally pre-treated with cannabinoid receptors’ blockers (CBs-) 1 (SR141716) and 2 (AM-630). Results: The sperm progressive motility, average path velocity (VAP), curvilinear velocity (VCL), straight-line velocity (VSL), amplitude of lateral head (ALH) and beat cross frequency (BCF) were reduced by THC but increased by vitamin C when compared to control. Vitamin C inhibited the THC-induced reduction in these parameters in the absence of CBs- 1 and 2, and even caused additional increases in progressive motility, VAP and VCL above the control levels with CBs-. Conclusion: In conclusion, vitamin C ameliorates THC-induced reduction in sperm motility in-vitro by modulation of sperm kinematics.

show abstract

Melatonin and vitamin C exacerbate Cannabis sativa-induced testicular damage when administered separately but ameliorate it when combined in rats

Abstract: This study further showed the gonadotoxic effect of C. sativa, which could be mediated by oxidative stress. It also showed that melatonin and vitamin C exacerbate C. sativa-induced testicular damage when administered separately but ameliorate it when combined in rats.

Cited by 29 publications

References 40 publications

Codeine-induced sperm DNA damage is mediated predominantly by oxidative stress rather than apoptosis

Codeine-induced sperm DNA damage is mediated predominantly by oxidative stress rather than apoptosis

Research Trends and Hotspots Analysis Related to the Effects of Xenobiotics on Glucose Metabolism in Male Testes

Vitamin C Ameliorates Tetrahydrocannabinol-Induced Spermatotoxicity In-Vitro

Contact Info

Product

Resources

About