Melatonin and Trolox ameliorate duodenal LPS-induced disturbances and oxidative stress

Fagundes, Diego Santos; Gonzálo, Sergio; Arruebo, M. P.; Plaza, Miguel Ángel; Murillo, María Divina

doi:10.1016/j.dld.2009.04.014

Cited by 15 publications

(14 citation statements)

References 37 publications

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“…In this study, genistein and quercetin reduced the amplitude of spontaneous contractions in the longitudinal and circular smooth muscle of the duodenum, but they did not modify the frequency. This result agrees with the effect of melatonin, an antioxidant, in the duodenum (14). Our results with genistein or quercetin agree with previous findings showing that flavonoids The values are the mean ± SEM.…”

Section: Discussionsupporting

confidence: 83%

Different mechanisms of actions of genistein and quercetin on spontaneous contractions of rabbit duodenum

Santos-Fagundes¹,

Grasa²,

Gonzálo³

et al. 2015

Rev Esp Enferm Dig

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Flavonoids are known to relax precontracted intestinal smooth muscle and delay intestinal transit or intestinal peristalsis. The aim of this study was to determine the effects of genistein and quercetin on spontaneous contractions of rabbit duodenum in vitro in an organ bath. Genistein and quercetin (0.1-10 µM) reduced the amplitude of spontaneous contractions in the longitudinal and circular smooth muscle of rabbit duodenum, but they did not modify the frequency. Bay K8644 (L-type Ca 2+ channel activator), apamin, charybdotoxin, and tetraetylammonium (K + channel blockers) reverted the inhibition of amplitude of spontaneous contractions induced by genistein in longitudinal and circular smooth muscle. H-89 (protein kinase A inhibitor) antagonized the reduction of the amplitude of spontaneous contractions induced by quercetin in longitudinal and circular smooth muscle of duodenum, while 2,5-dideoxiadenosine (adenylyl cyclase inhibitor) reverted only the reduction of the amplitude in circular smooth muscle. In conclusion, genistein and quercetin reduce the spontaneous contractions in the duodenum by different mechanisms of actions. The effect of genistein would be mediated by Ca 2+ and K + channels, while the effect of quercetin would be mediated by cAMP and protein kinase A.

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Section: Discussionsupporting

confidence: 83%

Different mechanisms of actions of genistein and quercetin on spontaneous contractions of rabbit duodenum

Santos-Fagundes¹,

Grasa²,

Gonzálo³

et al. 2015

Rev Esp Enferm Dig

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“…A growing body of data document that a pro‐inflammatory and oxidative condition is related to the pathogenesis and the progression of endotoxin‐induced septic shock. It is confirmed by the fact that anti‐oxidants have therapeutic effects in LPS‐induced sepsis and its consequent gastrointestinal disturbances 15,28,36 . The beneficial effect of melatonin in the LPS‐induced motility disorders is associated with a reduction in lipid peroxidation and MAPK activation 28 .…”

Section: Discussionmentioning

confidence: 90%

Inhibition of p38 MAPK improves intestinal disturbances and oxidative stress induced in a rabbit endotoxemia model

Gonzálo

Grasa

Arruebo

et al. 2009

Neurogastroenterology &Amp; Motility

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“…Gastrointestinal movement disturbance occurs commonly with IBDs 27 . The LPS‐induced septic ileus model is often used in experimental study 28–30 . Based on our previous work, 22 GI motility disorder was replicated by LPS i.p.…”

Section: Discussionmentioning

confidence: 99%

A novel CB receptor GPR55 and its ligands are involved in regulation of gut movement in rodents

Lin

Yuece

et al. 2011

Neurogastroenterology Motil

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Melatonin and Trolox ameliorate duodenal LPS-induced disturbances and oxidative stress

Cited by 15 publications

References 37 publications

Different mechanisms of actions of genistein and quercetin on spontaneous contractions of rabbit duodenum

Different mechanisms of actions of genistein and quercetin on spontaneous contractions of rabbit duodenum

Inhibition of p38 MAPK improves intestinal disturbances and oxidative stress induced in a rabbit endotoxemia model

A novel CB receptor GPR55 and its ligands are involved in regulation of gut movement in rodents

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