Melatonin and nitric oxide: Dual players inhibiting hazardous metal toxicity in soybean plants via molecular and antioxidant signaling cascades

Imran, Muhammad; Khan, Abdul Latif; Mun, Bong-Gyu; Bilal, Saqib; Shaffique, Shifa; Kwon, Eun-Hae; Kang, ⋅Sang-Mo; Yun, Byung‐Wook; Lee, In‐Jung

doi:10.1016/j.chemosphere.2022.136575

Cited by 30 publications

(13 citation statements)

References 97 publications

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“…On the other hand, neither did the extremely high levels of added Pb. Such a finding indicates the tolerance that the test plant exhibits towards Pb stress, in line with the findings for other plants ( Chrysopogon zizanioides —[ 26 ]; Tagetes erecta —[ 27 ]; soybean—[ 28 ]).…”

Section: Discussionsupporting

confidence: 86%

The Response of Purslane (Portulaca oleracea) to Soil-Added Pb: Is It Suitable as a Potential Phytoremediation Species?

Thalassinos

Petropoulos

Antoniadis

2023

Toxics

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Soils with high lead (Pb) levels can be decontaminated with the use of tolerant plants. Their effectiveness may be increased with added soil N due to boosted plant vigor, but such an agronomic practice has not been widely reported so far. In this work, purslane (Portulaca oleracea) was tested in a pot experiment as a potential phytoremediation species using soil spiked with Pb at doses of 0, 150, 300, 600, and 900 mg kg−1 (referred to as Pb(0), Pb(150), Pb(300), Pb(600), and Pb(900), respectively) with added N (referred to as N(1); at 300 kg N ha−1) and without added N (N(0)). We found that added Pb did not cause any adverse effects on plant growth (height, and aerial and root dry biomass) and physiological parameters, which were boosted with added N. Lead plant concentration and uptake significantly increased with added N, a finding that confirms our hypothesis. The number of necessary harvests of purslane in order to reduce soil Pb to half its initial concentration was also calculated and found to decrease with added N, being 131 at Pb(900)N(1). Although results indicate the potential of purslane as a phytoremediation species, further research is needed under real field conditions.

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Section: Discussionsupporting

confidence: 86%

The Response of Purslane (Portulaca oleracea) to Soil-Added Pb: Is It Suitable as a Potential Phytoremediation Species?

Thalassinos

Petropoulos

Antoniadis

2023

Toxics

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“…Similar results were shown by Shahzad et al 71 where B. amyloliquefaciens RWL‐1 augmented crop growth and reduced heavy metal accumulation through improving carbohydrate levels (glucose, sucrose, fructose, and raffinose), enhancing amino acid regulation, and augmenting antioxidant levels (POD, PPO, and GHS) 71 . The role of the melatonin synthesis of EH143 in augmenting stress tolerance is aligned with the findings of Imran et al 72 who showed that the application of melatonin reduced Cd and Pb mobilization by increasing the uptake of Ca 2+ and K + and increasing the exudation of organic acids into the rhizosphere 72 . Moreover, it was found that inoculation of P. aeruginosa and B. gladioli alleviated Cd‐induced toxicity, which was manifested through enhanced phenolic compound levels and osmolytes 66 .…”

Section: Discussionsupporting

confidence: 82%

“…71 The role of the melatonin synthesis of EH143 in augmenting stress tolerance is aligned with the findings of Imran et al 72 who showed that the application of melatonin reduced Cd and Pb mobilization by increasing the uptake of Ca 2+ and K + and increasing the exudation of organic acids into the rhizosphere. 72 Moreover, it was found that inoculation of P. aeruginosa and B. gladioli alleviated Cdinduced toxicity, which was manifested through enhanced phenolic compound levels and osmolytes. 66 A range of metabolites, including amino acids, fatty acids, carbohydrates, flavonoids, and phenols, as well as Cd detoxification, were controlled by Bacillus subtilis inoculation on alfalfa 73 and by inoculation of Pseudomonas putida in Rumex palustris, Alcea aucheri, and Arabidopsis thaliana.…”

Section: Discussionsupporting

confidence: 75%

Novel melatonin‐producing Bacillus safensis EH143 mitigates salt and cadmium stress in soybean

Kwon,

Adhikari,

Imran

et al. 2024

Journal of Pineal Research

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Recently, microorganism and exogenous melatonin application has been recognized as an efficient biological tool for enhancing salt tolerance and heavy metal detoxification in agriculture crops. Thus, the goal of this study was to isolate and evaluate a novel melatonin‐producing plant growth promoting bacterium. With high‐throughput whole genome sequencing, phytohormone measurements, expression profiling, and biochemical analysis, we can identify a novel PGPB that produces melatonin and unravel how it promotes soybean growth and development and protects against salt and Cd stress. We identify the melatonin synthesis pathway (tryptophan→tryptamine→serotonin melatonin) of the halotolerant (NaCl > 800 mM) and heavy metal‐resistant (Cd >3 mM) rhizobacterium Bacillus safensis EH143 and use it to treat soybean plants subjected to Cd and NaCl stresses. Results show that EH143 will highly bioaccumulate heavy metals and significantly improve P and Ca2+ uptake and the K+/Na+ (93%↑under salt stress) ratio while reducing Cd uptake (49% under Cd stress) in shoots. This activity was supported by the expression of the ion regulator HKT1, MYPB67, and the calcium sensors CDPK5 and CaMK1 which ultimately led to increased plant growth. EH143 significantly decreased ABA content in shoots by 13%, 20%, and 34% and increased SA biosynthesis in shoots by 14.8%, 31%, and 48.2% in control, salt, and Cd‐treated plants, upregulating CYP707A1 and CYP707A2 and PAL1 and ICS, respectively. The melatonin content significantly decreased along with a reduced expression of ASMT3 following treatment with EH143; moreover, reduced expression of peroxidase (POD) and superoxide dismutase (SOD) by 134.5% and 39% under salt+Cd stress, respectively and increased level of total amino acids were observed. Whole‐genome sequencing and annotation of EH143 revealed the presence of the melatonin precursor tryptophan synthase (trpA, trpB, trpS), metal and other ion regulators (Cd: cadA, potassium: KtrA and KtrB, phosphate: glpT, calcium: yloB, the sodium/glucose cotransporter: sgIT, and the magnesium transporter: mgtE), and enzyme activators (including the siderophore transport proteins yfiZ and yfhA, the SOD sodA, the catalase katA1, and the glutathione regulator KefG) that may be involved in programming the plant metabolic system. As a consequence, EH143 treatment significantly reduced the contents of lipid peroxidation (O2‐, MDA, and H2O2) up to 69%, 46%, and 29% in plants under salt+Cd stress, respectively. These findings suggest that EH143 could be a potent biofertilizer to alleviate NaCl and Cd toxicity in crops and serve as an alternative substitute for exogenous melatonin application.

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“…In stress signal transduction, CDPK could be activated by ROS, which can phosphorylate and activate the NADPH-mediated respiratory burst oxidase homologs (RBOHs), to trigger downstream defense response for better adaptability under unfavorable conditions [ 42 ]. It has been reported that MT also activates the calcium-dependent protein kinase (CDPK) via Ca 2+ signaling [ 43 ]. In response to higher H 2 O 2 accumulation in tomatoes leaves; the foliar application of MT resulted in higher expression of CDPK biosynthesis genes ( CDPK1 and CDPK2 ), which gradually increased the Ca 2+ signaling to regulate diverse physiological processes to improve redox balance and stress adaptation [ 44 ].…”

Section: Coordination Of Mt and Secondary Messengers Under Abiotic St...mentioning

confidence: 99%

Phytomelatonin: A key regulator of redox and phytohormones signaling against biotic/abiotic stresses

et al. 2023

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Melatonin and nitric oxide: Dual players inhibiting hazardous metal toxicity in soybean plants via molecular and antioxidant signaling cascades

Cited by 30 publications

References 97 publications

The Response of Purslane (Portulaca oleracea) to Soil-Added Pb: Is It Suitable as a Potential Phytoremediation Species?

The Response of Purslane (Portulaca oleracea) to Soil-Added Pb: Is It Suitable as a Potential Phytoremediation Species?

Novel melatonin‐producing Bacillus safensis EH143 mitigates salt and cadmium stress in soybean

Phytomelatonin: A key regulator of redox and phytohormones signaling against biotic/abiotic stresses

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