Melatonin and Mammary Pathological Growth

Cos, Samuel; Sánchez‐Barceló, Emilio J.

doi:10.1006/frne.1999.0194

Cited by 162 publications

(171 citation statements)

References 134 publications

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“…In these studies, optimal blastocyst rates were achieved when physiological melatonin rates (Cos and Sanchez-Barceló, 2000), which varied from 10 -9 M to 10 -6 M, were added to culture media. In the present study, a concentration of melatonin close to physiological levels (10 -9 M) was tested because elevated concentrations of melatonin negatively affect follicle survival in culture (Adriaens et al, 2006).…”

Section: Resultsmentioning

confidence: 99%

Melatonin in maturation media fails to improve oocyte maturation, embryo development rates and DNA damage of bovine embryos

Takada

Martins

Mingoti

et al. 2010

Sci. agric. (Piracicaba, Braz.)

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Melatonin (MEL) acts as a powerful scavenger of free radicals and direct gonadal responses to melatonin have been reported in the literature. Few studies, however, have evaluated the effect of MEL during in vitro maturation (IVM) on bovine embryos. This study tested the addition of MEL to maturation medium (MM) with no gonadotropins on nuclear maturation and embryo development rates and the incidence of DNA damage in resulting embryos. Cumulus-oocyte complexes were aspirated from abattoir ovaries and cultured in MM (TCM-199 medium supplemented with 10% fetal calf serum -FCS) at 39°C and 5% CO 2 in air. After 24 hours of culture in MM with 0.5 μg mL -1 FSH and 5.0 μg mL -1 LH; 10 -9 M MEL) or 10 -9 M MEL, 0.5 μg mL -1 FSH and 5.0 μg mL -1 LH, the oocytes were stained with Hoechst 33342 to evaluate nuclear maturation rate. After in vitro fertilization and embryo culture, development rates were evaluated and the blastocysts were assessed for DNA damage by Comet assay. There was no effect of melatonin added to the MM, alone or in combination with gonadotropins, on nuclear maturation, cleavage and blastocyst rates. These rates ranged between 88% to 90%, 85% to 88% and 42% to 46%, respectively. The extent of DNA damage in embryos was also not affected by MEL supplementation during IVM. The addition of 10 -9 M MEL to the MM failed to improve nuclear maturation and embryo development rates and the incidence of DNA damage in resulting embryos, but was able to properly substitute for gonadotropins during IVM. Key words: comet assay, embryos, gonadotropins, in vitro fertilization, melatonin Melatonina no meio de maturação não melhorou as taxas de maturação dos ovócitos, de desenvolvimento embrionário e a fragmentação do DNA dos embriões bovinos RESUMO: Melatonin (MEL) atua como um potente redutor de radicais livres. Efeito direto da MEL na função gonadal também foi observado. Existem poucos estudos relacionados ao efeito da MEL durante a maturação no desenvolvimento embrionário in vitro. Avaliou-se a adição de MEL no meio de maturação (sem gonadotrofinas) nas taxas de maturação nuclear e de desenvolvimento embrionário e na incidência de fragmentação do DNA nos embriões produzidos in vitro. Complexos cumulus-ovócitos foram aspirados de ovários provenientes de matadouros e cultivados em meio de maturação (Meio TCM-199 suplementado com 10% de soro fetal bovino) a 39°C e 5% CO 2 em ar. Após 24 horas de cultivo em meio de maturação com 0,5 μg mL -1 de FSH e 5,0 μg mL -1 de LH; 10 -9 M de MEL ou 10 -9 M de MEL, 0,5 μg mL -1 de FSH e 5,0 μg mL -1 de LH, os ovócitos foram corados com Hoechst 33342 para avaliação da taxa de maturação nuclear. A fragmentação do DNA dos blastocistos foi avaliada pela técnica do Cometa. Não houve efeito da MEL adicionada ao meio de maturação, com ou sem gonadotrofinas, nas taxas de maturação nuclear, clivagem e blastocistos. Os percentuais variaram entre 88% a 90%, 85% a 88% e 42% a 46%, respectivamente. O grau de fragmentação do DNA também não foi afetado pela adição de MEL ao meio de matur...

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Section: Resultsmentioning

confidence: 99%

Melatonin in maturation media fails to improve oocyte maturation, embryo development rates and DNA damage of bovine embryos

Takada

Martins

Mingoti

et al. 2010

Sci. agric. (Piracicaba, Braz.)

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“…In the absence of estradiol (E2), the inactive receptor is complexed with a variety of proteins that block its ability to interact with DNA whereas in the presence of E2, the receptor undergoes a conformational change that allows its binding to coactivators, initiating the transcription of target genes. Melatonin downregulates ER expression by suppressing ER gene transcription, resulting in a reduction in ER mRNA and protein levels [125,126]. Melatonin also inhibits the mitogenic effects of E2 in cancer cells by blocking its ability to stimulate binding of ER to DNA: melatonin inhibits binding of the E2-ER complex to the estrogen response element (ERE) on DNA [38].…”

Section: Anti-estrogenic Activitiesmentioning

confidence: 99%

Melatonin-Induced Oncostasis, Mechanisms and Clinical Relevance

Cardinali¹,

Escames²,

Acuña-Castroviejo³

et al. 2016

J Integr Oncol

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“…In such perspective, it might be worth to better characterize this issue with the aim of designing efficient ionic channel inhibitors/modulators that may potentially affect cancer therapy. Functional relationship exists between resting membrane potential, K þ channel type expression and cell functions such as proliferation and differentiation (Asher et al, 2010;Cos and Sánchez-Barceló, 2003;Enomoto et al, 1986;Wang, 2004). Resting membrane potential is typically more depolarized in undifferentiated respect to differentiated cells (O'Grady and Lee, 2005;Pardo, 2004) and in cancer cells respect to terminally differentiated cells (Kunzelmann, 2005).…”

Section: Introductionmentioning

confidence: 99%

Melatonin affects voltage-dependent calcium and potassium currents in MCF-7 cell line cultured either in growth or differentiation medium

Squecco

Tani

Zecchi‐Orlandini

et al. 2015

European Journal of Pharmacology

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Chemical compounds studied in this article:α-Dendrotoxin (PubChem SID: 135651983) Chromanol (PubChem CID: 92890) Iberiotoxin (PubChem CID: 16132435) Melatonin (PubChem CID: 896) Nifedipine (PubChem CID: 4485) a b s t r a c t Big efforts have been dedicated up to now to identify novel targets for cancer treatment. The peculiar biophysical profile and the atypical ionic channels activity shown by diverse types of human cancers suggest that ion channels may be possible targets in cancer therapy. Earlier studies have shown that melatonin exerts an oncostatic action on different tumors. In particular, it was shown that melatonin was able to inhibit growth/viability and proliferation, to reduce the invasiveness and metastatic properties of human estrogen-sensitive breast adenocarcinoma MCF-7 cell line cultured in growth medium, with substantial impairments of epidermal growth factor (EGF) and Notch-1-mediated signaling. The purpose of this work was to evaluate on MCF-7 cells the possible effects of melatonin on the biophysical features known to have a role in proliferation and differentiation, by using the patch-clamp technique. Our results show that in cells cultured in growth as well as in differentiation medium melatonin caused a hyperpolarization of resting membrane potential paralleled by significant changes of the inward Ca 2 þ currents (T-and L-type), outward delayed rectifier K þ currents and cell capacitance. All these effects are involved in MCF-7 growth and differentiation. These findings strongly suggest that melatonin, acting as a modulator of different voltage-dependent ion channels, might be considered a new promising tool for specifically disrupting cell viability and differentiation pathways in tumour cells with possible beneficial effects on cancer therapy.

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Melatonin and Mammary Pathological Growth

Cited by 162 publications

References 134 publications

Melatonin in maturation media fails to improve oocyte maturation, embryo development rates and DNA damage of bovine embryos

Melatonin in maturation media fails to improve oocyte maturation, embryo development rates and DNA damage of bovine embryos

Melatonin-Induced Oncostasis, Mechanisms and Clinical Relevance

Melatonin affects voltage-dependent calcium and potassium currents in MCF-7 cell line cultured either in growth or differentiation medium

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