Melatonin and glycolytic protein interactions are related to yeast fermentative capacity

Transcriptomic Insights into the Effect of Melatonin in Saccharomyces cerevisiae in the Presence and Absence of Oxidative Stress

“…Indeed, it has been reported that hypoxia induces the formation of foci containing glycolytic enzymes, which increases the incorporation of carbon into pyruvate and oxaloacetate [ 91 ]. Recently, melatonin has been shown to bind a complex of glycolytic enzymes [ 17 ], and this binding would be related to yeast fermentative capacity [ 18 ]. Several glycolytic genes found in this complex, such as PYC2 and TDH3 , were upregulated by melatonin in non-stressed cells ( Figure 1 B,E, Dataset S1 ).…”

“…Recent studies have demonstrated a protective function of melatonin against oxidative stress and UV radiation [ 14 , 15 , 16 ] and indicated a hypothetical role as a signaling molecule [ 7 ]. The recent finding that melatonin interacts with glycolytic proteins during alcoholic fermentation reinforces the latter role, which seems to be linked to fermentative metabolism [ 17 , 18 ].…”

Effect of Several Nutrients and Environmental Conditions on Intracellular Melatonin Synthesis in Saccharomyces cerevisiae

“…Rodriguez-Naranjo and collaborators [ 22 ] highlighted the role of yeast, specifically Saccharomyces cerevisiae , in the production of melatonin during the winemaking process. However, not only does S. cerevisiae produce melatonin but also several non- Saccharomyces strains are able to synthetize melatonin during alcoholic fermentation [ 14 , 15 , 16 , 23 , 24 , 25 ]. Melatonin production depends on the availability of tryptophan, which is a precursor [ 14 , 16 , 26 ], but also some tryptophan derivatives (N-acetyl serotonin or 5-methoxytryptamine) can be used as melatonin precursors [ 27 ].…”

“…Melatonin has been described in the extracellular medium during the exponential phase of growth (between the first and second day) [ 14 , 15 , 17 , 23 , 24 , 26 ] and at the end of alcoholic fermentation (at the stationary phase) [ 25 ] depending on the yeast species involved in the fermentation. Nevertheless, few studies about intracellular melatonin have been published.…”

“…Nevertheless, few studies about intracellular melatonin have been published. Some authors have provided evidence that melatonin is present intracellularly after precursor pulses [ 16 , 18 , 27 ], and also, in oenological conditions, both in Saccharomyces [ 17 ] and in non- Saccharomyces yeasts [ 16 , 24 ], although few strains have been studied.…”