Melatonin and calcium act synergistically to enhance the coproduction of astaxanthin and lipids in Haematococcus pluvialis under nitrogen deficiency and high light conditions

Cui, Jing; Yu, Chen; Zhong, Du-bo; Zhao, Yongteng; Yu, Xuya

doi:10.1016/j.biortech.2020.123069

Cited by 56 publications

(17 citation statements)

References 49 publications

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“…It should be noted that the total fatty acid production was significantly improved by ~60% (Figure 5), although the change in cellular PUFA content was small. Similarly, Cui et al [32] reported that the combination of melatonin and CaCl 2 promoted the PUFA ratio among the total fatty acids in H. pluvialis LUGU. Salama et al [35] observed the promotion of PUFA biosynthesis in C. mexicana GU732420 and S. obliquus HM103382 in the presence of the phytohormone indole-3-acetic acid.…”

Section: Astaxanthin and Fatty Acid Productionmentioning

confidence: 86%

“…They also reported a 1.3-fold improvement in lipid productivity with the same rac-GR24 dosage under nitrogen-deficient conditions [15]. Compared to the control group, melatonin improved the lipid content of H. pluvialis LUGU by 49.5% under photoautotrophic conditions [32], and salicylic acid increased the total fatty acid content of H. pluvialis 192.80 by 36.0% under photoheterotrophic conditions [30]. The combination of melatonin and nitrogen deficiency improved the lipid content in M. sp.…”

Section: Astaxanthin and Fatty Acid Productionmentioning

confidence: 92%

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Enhancement of Astaxanthin and Fatty Acid Production in Haematococcus pluvialis Using Strigolactone

et al. 2022

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Improving the production rate of high-value nutraceutical compounds, such as astaxanthin and polyunsaturated fatty acids (PUFAs), is important for the commercialization of Haematococcus pluvialis biorefineries. Here, the effects of a phytohormone, strigolactone analog rac-GR24, on cell growth and astaxanthin and fatty acid biosynthesis in H. pluvialis were investigated. Four concentrations (2, 4, 6, and 8 µM) of rac-GR24 were initially added during 30 days of photoautotrophic cultivation. The addition of rac-GR24 improved cell number density and chlorophyll concentration in H. pluvialis cultures compared to the control; the optimal concentration was 8 µM. Despite a slightly reduced astaxanthin content of 30-d-old cyst cells, the astaxanthin production (26.1 ± 1.7 mg/L) improved by 21% compared to the rac-GR24-free control (21.6 ± 1.5 mg/L), owing to improved biomass production. Notably, at the highest dosage of 8 µM rac-GR24, the total fatty acid content of the treated H. pluvialis cells (899.8 pg/cell) was higher than that of the untreated cells (762.5 pg/cell), resulting in a significant increase in the total fatty acid production (361.6 ± 48.0 mg/L; 61% improvement over the control). The ratio of PUFAs, such as linoleic (C18:2) and linolenic (C18:3) acids, among total fatty acids was high (41.5–44.6% w/w) regardless of the rac-GR24 dose.

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Section: Astaxanthin and Fatty Acid Productionmentioning

confidence: 86%

Section: Astaxanthin and Fatty Acid Productionmentioning

confidence: 92%

Enhancement of Astaxanthin and Fatty Acid Production in Haematococcus pluvialis Using Strigolactone

et al. 2022

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“…High H. pluvialis biomass can be obtained through the mixotrophic mode of cultivation utilizing acetic acid or acetate [35]. In the cultivation of H. pluvialis, the astaxanthin accumulation was induced by using an organic carbon source supplemented with 100 mM potassium acetate [13].…”

Section: Effect Of Carbon Sourcesmentioning

confidence: 99%

A Review on Haematococcus pluvialis Bioprocess Optimization of Green and Red Stage Culture Conditions for the Production of Natural Astaxanthin

et al. 2021

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As the most recognizable natural secondary carotenoid astaxanthin producer, the green microalga Haematococcus pluvialis cultivation is performed via a two-stage process. The first is dedicated to biomass accumulation under growth-favoring conditions (green stage), and the second stage is for astaxanthin evolution under various stress conditions (red stage). This mini-review discusses the further improvement made on astaxanthin production by providing an overview of recent works on H. pluvialis, including the valuable ideas for bioprocess optimization on cell growth, and the current stress-exerting strategies for astaxanthin pigment production. The effects of nutrient constituents, especially nitrogen and carbon sources, and illumination intensity are emphasized during the green stage. On the other hand, the significance of the nitrogen depletion strategy and other exogenous factors comprising salinity, illumination, and temperature are considered for the astaxanthin inducement during the red stage. In short, any factor that interferes with the cellular processes that limit the growth or photosynthesis in the green stage could trigger the encystment process and astaxanthin formation during the red stage. This review provides an insight regarding the parameters involved in bioprocess optimization for high-value astaxanthin biosynthesis from H. pluvialis.

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“…Furthermore, nitrogen concentration can also markedly affect the fatty acid composition (Sahin et al, 2019). It determines the quality of biofuel such as combustion efficiency, cold-flow properties and viscosity (Cui et al, 2020;Andeden et al, 2021), which is rarely considered in current studies.…”

Section: Introductionmentioning

confidence: 99%

Effect of Nitrogen Concentration on the Alkalophilic Microalga Nitzschia sp. NW129-a Promising Feedstock for the Integrated Production of Lipids and Fucoxanthin in Biorefinery

et al. 2022

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Microalgae are considered promising resources for producing a variety of high-value-added products, especially for lipids and pigments. Alkalophilic microalgae have more advantages than other microalgae when cultured outdoors on a large scale. The present study investigated the comprehensive effects of different nitrogen concentrations on fucoxanthin (Fx), lipids accumulation and the fatty acid profile of the alkaliphilic microalgae Nitzschia sp. NW129 to evaluate the potential for simultaneous production of Fx and biofuels. Fx and Lipids amassed in a coordinated growth-dependent manner in response to various concentrations, reaching 18.18 mg g–1 and 40.67% dry weight (DW), respectively. The biomass of Nitzschia sp. NW129 was 0.58 ± 0.02 g L–1 in the medium at the concentration of 117.65 mM. The highest productivities of Fx (1.44 mg L–1 d–1) and lipid (19.95 ± 1.29 mg L–1 d–1) were obtained concurrently at this concentration. Furthermore, the fatty acid methyl esters revealed excellent biofuel properties with an appropriate value of the degree unsaturation (49.97), cetane number (62.72), and cold filter plugging point (2.37), which met the European standards for biofuel production (EN14214). These results provided a reliable strategy for further industrialization and comprehensive production of biofuel and Fx by using the alkaliphilic microalgal Nitzschia sp. NW129.

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Melatonin and calcium act synergistically to enhance the coproduction of astaxanthin and lipids in Haematococcus pluvialis under nitrogen deficiency and high light conditions

Cited by 56 publications

References 49 publications

Enhancement of Astaxanthin and Fatty Acid Production in Haematococcus pluvialis Using Strigolactone

Enhancement of Astaxanthin and Fatty Acid Production in Haematococcus pluvialis Using Strigolactone

A Review on Haematococcus pluvialis Bioprocess Optimization of Green and Red Stage Culture Conditions for the Production of Natural Astaxanthin

Effect of Nitrogen Concentration on the Alkalophilic Microalga Nitzschia sp. NW129-a Promising Feedstock for the Integrated Production of Lipids and Fucoxanthin in Biorefinery

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