Melatonin ameliorates imidacloprid-induced intestinal injury by negatively regulating the PGN/P38MAPK pathway in the common carp (Cyprinus carpio)

Miao, Zhiruo; Miao, Zhiying; Liu, Min; Xu, Shiwen

doi:10.1016/j.fsi.2022.11.018

Cited by 22 publications

(6 citation statements)

References 86 publications

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“…We used the TRIzol reagent to extract total RNA from 0.1 g of liver tissue and LMH cells in different treatment groups. 42 Then, we synthesized cDNA using the BioRT Master HiSensi cDNA first strand synthesis kit and the miRNA first strand cDNA synthesis kit (Hangzhou Biotechnology, China). Subsequently, we tested the expression of target genes using the LightCycler® 480 II Assay System (Roche, Basel, Switzerland).…”

Section: Methodsmentioning

confidence: 99%

Selenium deficiency caused hepatitis in chickens via the miR-138-5p/SelM/ROS/Ca²⁺ overload pathway induced by hepatocyte necroptosis

Sun,

Zhang,

Shi

et al. 2023

Food Funct.

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Section: Methodsmentioning

confidence: 99%

Selenium deficiency caused hepatitis in chickens via the miR-138-5p/SelM/ROS/Ca²⁺ overload pathway induced by hepatocyte necroptosis

Sun,

Zhang,

Shi

et al. 2023

Food Funct.

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“…Fixed broiler breast muscle tissues were dehydrated with alcohol, embedded in paraffin, and sectioned into 5 μm thickness slices. The slices were dewaxed, hydrated, stained with H&E, observed, and photographed with an optical microscope. , …”

Section: Methodsmentioning

confidence: 99%

“…The slices were dewaxed, hydrated, stained with H&E, observed, and photographed with an optical microscope. 25,26 2.5. Immunohistochemistry (IHC).…”

Section: Hematoxylin and Eosin (Hande) Stainingmentioning

confidence: 99%

Low-Se Diet Increased Mitochondrial ROS to Suppress Myoblasts Proliferation and Promote Apoptosis in Broilers via miR-365-3p/SelT Signaling Axis

Wu,

Xu,

Yang

et al. 2023

J. Agric. Food Chem.

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microRNA (miRNA) controls the post-transcriptional translation of mRNA to affect the expression of many genes participating in functional interaction pathways. Selenoproteins are characterized by their antioxidant activity, wherein selenoprotein T (SelT) is an essential membrane-bound selenoprotein serving as a guardian of intracellular homeostasis. During muscle development and regeneration, myoblasts enter the cell cycle and rapidly proliferate. However, the role of SelT in muscle development and selenium (Se) deficiency-induced muscle damage remains poorly investigated. This study established Se deficient broiler models, chicken embryos models, and cultured chicken primary myoblasts in vitro. We showed that Se deficiency induced skeletal muscle damage in broilers, promoted miR-365-3p expression, and downregulated the level of SelT, significantly. The absence of SelT led to the accumulation of mitochondrial superoxide and downregulated mitochondrial dynamics gene expression, which, in turn, induced the disruption of mitochondria potential and blocked the oxidative phosphorylation (OXPHOS) process. Limited ATP production rate caused by mitochondrial ROS overproduction went along with cell cycle arrest, cell proliferation slowness, and myocyte apoptosis increase. Using Mito-TEMPO for mitochondrial ROS elimination could effectively mitigate the above adverse reactions and significantly restore the proliferation potential of myoblasts. Moreover, we identified miR-365-3p, a miRNA that targeted SelT mRNA to inhibit myoblast proliferation by disrupting intracellular redox balance. The omics analysis results showed that Se deficiency led to the significant enrichment of "cell cycle", "oxidative stress response", and "oxidative phosphorylation" pathway genes. Finally, we proved that the effect of the miR-365-3p/SelT signaling axis on muscle development did exist in the chicken embryo stage. In summary, our findings revealed that miR-365-3p was involved in broiler skeletal muscle damage in Se deficiency by targeting SelT, and SelT, serving as an intracellular homeostasis guardian, resisted mitochondrial oxidative stress, and protected ATP generation, promoting myoblast proliferation and inhibiting apoptosis. This study provides an attractive target for the cultivated meat industry and regenerative medicine.

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“…The western blot analysis was performed according to the previous studies, 43,44 500 μl cell lysates and 5 μl protease inhibitor mixtures were added to cells of different treatment groups to extract cellular proteins. After electrophoresis, protein was shifted to the nitrocellulose membrane, the membrane was blocked in blocking buffer (TBST containing 5% skimmed milk) at 37 C for 2 h, and then mixed with the primary antibody of p-ASK1 (1:1000, Bioss, Beijing, China), p-JNK (1:1000, Wanlei, Shenyang, China), Bcl-2 (1:300), Bcl-2-Associated X (Bax, 1:500), Cyt-C (1:1500, Wanlei, Shenyang, China), Caspase 9 (1:500, Wanlei, Shenyang, China), Caspase 3 (1:500) and β-actin Except p-ASK1 and p-JNK, Cyt-C, Caspase 9 and β-actin, all primary antibodies came from our lab.…”

Section: Protein Extraction and Western Blotting Analysismentioning

confidence: 99%

Eucalyptol antagonized the apoptosis and immune dysfunction of grass carp hepatocytes induced by tetrabromobisphenol A by regulating ROS/ASK1/JNK pathway

Sun

Liu

Zhu

et al. 2023

Environmental Toxicology

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Tetrabromobisphenol A (TBBPA) is a common environmental pollutant which has multi‐organ toxicity to mammals. Eucalyptol (EUC) has super antioxidant biological activity. However, in this experimental study, we probed into the mechanism of toxic of TBBPA exposure on Grass carp hepatocytes (L8824 cells) and the antagonistic impact of EUC on TBBPA. We treated L8824 cells with 8 μg/ml TBBPA and/or 20 μM EUC for 24 h in this test research. The experiment results suggested that TBBPA exposure induced elevated levels of reactive oxygen species (ROS), led to oxidative stress, decreased SOD and CAT activities, decreased GSH and T‐AOC contents, exacerbated MDA accumulation, activated ASK1/JNK signaling pathway, and further increased the contents of mitochondrial dependent apoptosis pathway related indicators (Cyt‐C, Bax, Caspase 9, Caspase 3), while Bcl‐2 expression decreased. In addition, TBBPA exposure induced increased expression of TNF‐α, IL‐6, IL‐1β, and decreased expression of IL‐2, IFN‐γ, Hepcidin, β‐defensin, LEAP2. The oxidative stress level, ASK1/JNK signal pathway expression level, apoptosis ratio and cellular immune function of cells exposed to EUC alone did not change significantly. Combined exposure of TBBPA and EUC significantly reduced the proportion of apoptosis and restored cellular immune function. Therefore, these results suggest that EUC can effectively antagonize TBBPA‐induced apoptosis and immune dysfunction of L8824 cells by regulating ROS/ASK1/JNK signaling pathway.

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Melatonin ameliorates imidacloprid-induced intestinal injury by negatively regulating the PGN/P38MAPK pathway in the common carp (Cyprinus carpio)

Cited by 22 publications

References 86 publications

Selenium deficiency caused hepatitis in chickens via the miR-138-5p/SelM/ROS/Ca²⁺ overload pathway induced by hepatocyte necroptosis

Selenium deficiency caused hepatitis in chickens via the miR-138-5p/SelM/ROS/Ca²⁺ overload pathway induced by hepatocyte necroptosis

Low-Se Diet Increased Mitochondrial ROS to Suppress Myoblasts Proliferation and Promote Apoptosis in Broilers via miR-365-3p/SelT Signaling Axis

Eucalyptol antagonized the apoptosis and immune dysfunction of grass carp hepatocytes induced by tetrabromobisphenol A by regulating ROS/ASK1/JNK pathway

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Melatonin ameliorates imidacloprid-induced intestinal injury by negatively regulating the PGN/P38MAPK pathway in the common carp (Cyprinus carpio)

Cited by 22 publications

References 86 publications

Selenium deficiency caused hepatitis in chickens via the miR-138-5p/SelM/ROS/Ca2+ overload pathway induced by hepatocyte necroptosis

Selenium deficiency caused hepatitis in chickens via the miR-138-5p/SelM/ROS/Ca2+ overload pathway induced by hepatocyte necroptosis

Low-Se Diet Increased Mitochondrial ROS to Suppress Myoblasts Proliferation and Promote Apoptosis in Broilers via miR-365-3p/SelT Signaling Axis

Eucalyptol antagonized the apoptosis and immune dysfunction of grass carp hepatocytes induced by tetrabromobisphenol A by regulating ROS/ASK1/JNK pathway

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Selenium deficiency caused hepatitis in chickens via the miR-138-5p/SelM/ROS/Ca²⁺ overload pathway induced by hepatocyte necroptosis

Selenium deficiency caused hepatitis in chickens via the miR-138-5p/SelM/ROS/Ca²⁺ overload pathway induced by hepatocyte necroptosis