Melatonin alleviates β-zearalenol and HT-2 toxin-induced apoptosis and oxidative stress in bovine ovarian granulosa cells

Yang, Fang; Lian, Li; Chen, Kunlin; Li, Chengmin; Wang, Yiru; Wang, Genlin

doi:10.1016/j.etap.2019.03.005

Cited by 34 publications

(25 citation statements)

References 50 publications

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“…The present study demonstrated that β-zearalenol and HT-2 can induce the accumulation of ROS in bovine ovarian granulosa cells. The results are in line with a previous study showing that β-zearalenol and HT-2 trigger oxidative stress in bovine GCs by increasing ROS production [21]. Moreover, ROS production could mediate endoplasmic reticulum (ER) stress [40,41].…”

Section: Discussionsupporting

confidence: 91%

“…The increase in mycotoxins-treated accumulation of intracellular ROS levels cause oxidative stress damage and apoptosis [21,27,38], which eventually result in a decline in fertility [39]. The present study demonstrated that β-zearalenol and HT-2 can induce the accumulation of ROS in bovine ovarian granulosa cells.…”

Section: Discussionsupporting

confidence: 48%

“…These GCs were kept at 38.5 • C in 5% CO 2 environment and the medium was replaced by fresh medium every 24 h [10]. GCs were identified by follicle-stimulating hormone receptor (FSHR) antibody (PA5-28764, Thermo Fisher Scientific, Waltham, MA, USA) using immunocytochemistry, as described in a previous study [21].…”

Section: Isolation Culture and Identification Of Bovine Gcsmentioning

confidence: 99%

“…The treatment concentration ranges of β-zearalenol and HT-2 toxin were 0-200 µM and 0-200 nM, respectively. Doses of β-zearalenol and HT-2 were selected based on previous studies [10,13,21].…”

Section: Cell Treatment and Cell Viability Analysismentioning

confidence: 99%

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Understanding the Toxin Effects of β-Zearalenol and HT-2 on Bovine Granulosa Cells Using iTRAQ-Based Proteomics

Yang

et al. 2020

Animals

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Zearalenone (ZEA) and T-2 are the most common mycotoxins in grains and can enter the animal and human food-chain and cause many health disorders. To elucidate the toxic response profile, we stimulated bovine granulosa cells (GCs) with β-zearalenol or HT-2. Using isobaric tags for relative and absolute quantification (iTRAQ)-based proteomic, 178 and 291 differentially expressed proteins (DEPs, fold change ≥ 1.3 and p-value < 0.05) in β-zearalenol and HT-2 groups were identified, respectively. Among these DEPs, there were 66 common DEPs between β-zearalenol and HT-2 groups. These 66 DEPs were associated with 23 biological processes terms, 14 molecular functions terms, and 19 cellular components terms. Most heat shock proteins (HSPs) were involved in the toxic response. Reactive oxygen species accumulation, the endoplasmic reticulum (ER)-stress related marker molecule (GRP78), and apoptosis were activated. β-zearalenol and HT-2 inhibited oestradiol (E2) production. These results emphasized the important function of HSPs, clarified oxidative stress, and demonstrated the caspase-3 signaling cascade involved in mycotoxin-treated toxic response, along with decreased E2 production. This study offers new insights into the toxicity of β-zearalenol and HT-2 on ovarian granulosa cells.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 48%

Section: Isolation Culture and Identification Of Bovine Gcsmentioning

confidence: 99%

Section: Cell Treatment and Cell Viability Analysismentioning

confidence: 99%

See 2 more Smart Citations

Understanding the Toxin Effects of β-Zearalenol and HT-2 on Bovine Granulosa Cells Using iTRAQ-Based Proteomics

Yang

et al. 2020

Animals

Self Cite

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“…CAT plays key roles in neutralizing harmful hydrogen peroxide from various sources [44]. CAT mRNA expression in ovarian granulosa cells (GCs) decreases following β-zearalenol and HT-2 treatment, but upregulates in bovine melatonin-treated GCs [45]. Melatonin treatment improves frozen-thawed semen quality, and increases blastocyst development rate and CAT transcript abundance of the bovine in vitro-produced embryos originating from melatonin-treated spermatozoa [46].…”

Section: Discussionmentioning

confidence: 99%

Effect of Melatonin on the In Vitro Maturation of Porcine Oocytes, Development of Parthenogenetically Activated Embryos, and Expression of Genes Related to the Oocyte Developmental Capability

Yang

Wang

Cui

et al. 2020

Animals

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Melatonin treatment can improve quality and in vitro development of porcine oocytes, but the mechanism of improving quality and developmental competence is not fully understood. In this study, porcine cumulus–oocyte complexes were cultured in TCM199 medium with non-treated (control), 10−5 M luzindole (melatonin receptor antagonist), 10−5 M melatonin, and melatonin + luzindole during in vitro maturation, and parthenogenetically activated (PA) embryos were treated with nothing (control), or 10−5 M melatonin. Cumulus oophorus expansion, oocyte survival rate, first polar body extrusion rate, mitochondrial distribution, and intracellular levels of reactive oxygen species (ROS) and glutathione of oocytes, and cleavage rate and blastocyst rate of the PA embryos were assessed. In addition, expression of growth differentiation factor 9 (GDF9), tumor protein p53 (P53), BCL2 associated X protein (BAX), catalase (CAT), and bone morphogenetic protein 15 (BMP15) were analyzed by real-time quantitative PCR. The results revealed that melatonin treatment not only improved the first polar body extrusion rate and cumulus expansion of oocytes via melatonin receptors, but also enhanced the rates of cleavage and blastocyst formation of PA embryos. Additionally, melatonin treatment significantly increased intraooplasmic level of glutathione independently of melatonin receptors. Furthermore, melatonin supplementation not only significantly enhanced mitochondrial distribution and relative abundances of BMP15 and CAT mRNA, but also decreased intracellular level of ROS and relative abundances of P53 and BAX mRNA of the oocytes. In conclusion, melatonin enhanced the quality and in vitro development of porcine oocytes, which may be related to antioxidant and anti-apoptotic mechanisms.

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Effective protective agents against the organ toxicity of T-2 toxin and corresponding detoxification mechanisms: A narrative review

Wang,

Sun,

Wang

et al. 2024

Animal Nutrition

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Melatonin alleviates β-zearalenol and HT-2 toxin-induced apoptosis and oxidative stress in bovine ovarian granulosa cells

Cited by 34 publications

References 50 publications

Understanding the Toxin Effects of β-Zearalenol and HT-2 on Bovine Granulosa Cells Using iTRAQ-Based Proteomics

Understanding the Toxin Effects of β-Zearalenol and HT-2 on Bovine Granulosa Cells Using iTRAQ-Based Proteomics

Effect of Melatonin on the In Vitro Maturation of Porcine Oocytes, Development of Parthenogenetically Activated Embryos, and Expression of Genes Related to the Oocyte Developmental Capability

Effective protective agents against the organ toxicity of T-2 toxin and corresponding detoxification mechanisms: A narrative review

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