Melatonin acts through different mechanisms to control oxidative stress and primordial follicle activation and survival during in vitro culture of bovine ovarian tissue

Silva, Bianca R.; Costa, Francisco C.; De Lima Neto, Miguel F.; Caetano Filho, Francisco F.; de Assis, Ernando I.T.; Aguiar, Francisco L.N.; Silva, Anderson W.B.; Martins, Solano D.; Araújo, Valdevane R.; Matos, Maria H.T.; Costa, José J.N.; Silva, José R.V.

doi:10.1016/j.domaniend.2023.106824

Cited by 4 publications

(1 citation statement)

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“…This characteristic has unveiled a new realm of possibilities, suggesting that besides its receptormediated hormonal actions, melatonin may also exert receptor-independent effects in regulating reproduction (Gao et al 2022). Melatonin critically scavenges oxygen free radicals during oocyte maturation and ovulation, mitigating the detrimental effects of reactive oxygen species (ROS) and protecting oocytes from oxidative damage (Silva et al 2024). Ovarian melatonin follows a seasonal pattern in carp, reaching its peak at spawning and this temporal variation shows a signi cant negative correlation with malondialdehyde (MDA) level, an indicator of intracellular oxidative stress (Hasan et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Correlation of testicular melatonin and cellular stress in an annual spermatogenic cycle of Clarias batrachus

Acharyya,

Hasan

2024

Preprint

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Melatonin, primarily produced by pineal gland, shows a rhythmic pattern of synthesis in response to environmental cues. Hitherto, the role of testicular melatonin in the regulation of annual spermatogenic cycle is indeterminate in any fish species. Present study aims to explore a correlation, for the first time, of melatonin with cellular stress levels in accordance to the rhythmic progression of spermatogenesis through six distinct reproductive phases of an annual gonadal cycle of catfish, Clarias batrachus under natural photo-thermal conditions. We assessed gonadosomatic index (GSI), testicular melatonin concentrations, relative percentage of haploid cells by using DNA dye in the seminiferous tubules, and different intracellular stress markers. The concentration of testicular melatonin displayed a distinct seasonal pattern, reaching their peak during the “phase of functional maturity”. Interestingly, the levels of 2’,7’–dichlorofluorescein [reactive oxygen species (ROS) marker], malondialdehyde (MDA) and RNS (total nitrate) were found significantly lowered at the verge of “mature state”. Correlation and LOESS regression analyses showed a striking positive relationship between testicular melatonin concentration and the activity of antioxidative enzymes, including superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione S-transferase (GST). Principal component (PC) analysis also identified the key components of “mature state” viz., GSI, testicular melatonin, haploid cell population (spermatid and spermatozoa), and the activity of enzymatic antioxidants. Cumulatively, melatonin may have a role in growth and maturation of spermatogenic cells by reducing the cellular stress to augment the quality of germ cells required for seed production in the culture of any fish species.

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Section: Introductionmentioning

confidence: 99%