Melanoma-targeted photodynamic therapy based on hypericin-loaded multifunctional P123-spermine/folate micelles

Oliveira, Ana Carolina Vieira de; Morais, Flávia Amanda Pedroso de; Campanholi, Katieli da Silva Souza; Bidóia, Danielle Lazarin; Balbinot, Rodolfo Bento; Nakamura, Celso V.; Caetano, Wilker; Hioka, Noboru; Monteiro, Odair dos Santos; Rocha, Cláudia Quintino da; Gonçalves, Renato Sonchini

doi:10.1016/j.pdpdt.2022.103103

Cited by 5 publications

(8 citation statements)

References 62 publications

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“…It is well known that the removal of some nanoparticles from circulation is thought to occur primarily through the phagocytic action of macrophages [ 55 , 56 ]. In this sense, considering the poor ability to uptake the nanoparticles by promastigote forms, the data on mortality appear to be more closely related to selectivity to BRA concentrations, i.e., to the relationship BRA/F127 micelle ratio, as observed also in previous works and inferred by oxidation assay [ 24 , 57 , 58 ]. The biological action of F127 Pluronic is more pronounced at lower concentrations of this copolymer, as they promote changes in the cell membrane.…”

Section: Resultssupporting

confidence: 63%

“…A451DE4). The extraction and fractionation procedures followed previously described methods [ 24 ]. Briefly, Arrabidaea brachiopoda roots (300 g) were dried and ground in a knife mill.…”

Section: Methodsmentioning

confidence: 99%

“…F127 is an ABA triblock copolymer comprised of poly(ethylene oxide) (EO) and poly(propylene oxide) (PO) blocks, which self-assemble in core-shell micelles above the critical micelle concentration (CMC) in an aqueous medium [ 23 ]. Using F127 copolymer as a drug delivery system allows the encapsulation of poorly water-soluble drugs, enhancing their bioavailability, providing controlled release over time, better cellular uptake, and a prolonged drug release effect on the therapeutic target [ 24 , 25 ]. Liquid formulations have garnered extensive utilization in the field of pharmaceutics owing to their heightened dosing flexibility, ease of ingestion, and rapid onset of therapeutic effects [ 26 ].…”

Section: Introductionmentioning

confidence: 99%

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Design of Liquid Formulation Based on F127-Loaded Natural Dimeric Flavonoids as a New Perspective Treatment for Leishmaniasis

da Costa,

Marques,

do Nascimento

et al. 2024

Pharmaceutics

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Infectious and Parasitic Diseases (IPD) remain a challenge for medicine due to several interconnected reasons, such as antimicrobial resistance (AMR). American tegumentary leishmaniasis (ATL) is an overlooked IPD causing persistent skin ulcers that are challenging to heal, resulting in disfiguring scars. Moreover, it has the potential to extend from the skin to the mucous membranes of the nose, mouth, and throat in both humans and various animals. Given the limited effectiveness and AMR of current drugs, the exploration of new substances has emerged as a promising alternative for ATL treatment. Arrabidaea brachypoda (DC). Bureau is a native Brazilian plant rich in dimeric flavonoids, including Brachydin (BRA), which displays antimicrobial activity, but still little has been explored regarding the development of therapeutic formulations. In this work, we present the design of a low-cost liquid formulation based on the use of Pluronic F127 for encapsulation of high BRA concentration (LF-B500). The characterization techniques revealed that BRA-loaded F127 micelles are well-stabilized in an unusual worm-like form. The in vitro cytotoxicity assay demonstrated that LF-B500 was non-toxic to macrophages but efficient in the inactivation of forms of Leishmania amazonensis promastigotes with IC50 of 16.06 µg/mL. The results demonstrated that LF-B500 opened a new perspective on the use of liquid formulation-based natural products for ATL treatment.

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Section: Resultssupporting

confidence: 63%

“…A451DE4). The extraction and fractionation procedures followed previously described methods [ 24 ]. Briefly, Arrabidaea brachiopoda roots (300 g) were dried and ground in a knife mill.…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Design of Liquid Formulation Based on F127-Loaded Natural Dimeric Flavonoids as a New Perspective Treatment for Leishmaniasis

da Costa,

Marques,

do Nascimento

et al. 2024

Pharmaceutics

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“…We recently demonstrated in vitro the photoinactivation of B 16 F 10 melanoma cells mediated by hypericin (HY) loaded P123-FA and P123-SN nanoparticles [17]. Our results revealed that SN and FA enhanced the selectivity of HY by cancer cells rather than healthy cells (HaCat and L-929).…”

Section: Introductionmentioning

confidence: 86%

Hypericin-loaded in modified theranostic liposomes nanoplatform: a preliminary in vivo study of targeting and diagnosis

de Morais,

Balbinot,

Bakoshi

et al. 2024

Preprint

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Modified theranostic liposomes was created by combining phospholipid 1,2-dipalmitoyl-sn-3-glycerol-phosphatidylcholine with two previously modified Pluronic® copolymers covalently linked with spermine and folic acid to carry and stabilize the photosensitizer compound hypericin. After physicochemical characterization, the photocytotoxicity was evaluated against different cancer and healthy cells presenting a strong photodynamic effect. The formulation exhibited no photoactivity without illumination and without hypericin. In vivo pharmacokinetics biodistribution examined the uptake and theranostic potential of this nanoformulation after its intravenous administration in animal models. Fluorescence images revealed the maximum fluorescence between 0.5-4 h post-tail vein injection, making it an appropriate period for photodynamic treatment. The fluorescence of the entire body was monitored for at least 3-days, indicating that the theranostic procedures can be performed within the 0.5-4 h range after administration, after which the intensity decreases, indicating a potent metabolic ability with no significant side effects. The fluorescence images of the main organs consistently showed a signal during the 1st day of its application. After 48 h, only residues of the modified theranostic formulation were detected in the lungs and thyroid. The promising pharmacokinetics observed in our preliminary studies highlight the potential of this system, making it a worthy candidate for further investigation with tumor models.

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“…[36,38,39]; (2) hydrophobic polymers, such as poly lactic-co-glycolic acid (PLGA), polycaprolactone (PCL), polylactic acid (PLA), etc. [36,[40][41][42]; (3) amphiphilic block copolymers, mainly including D-alphatocopheryl polyethylene glycol succinate (TPGS), Soluplus ® , and Pluronic ® (F127, F68, and P123) [13,[43][44][45][46][47][48][49]. The classification and structures of the aforementioned polymers are shown in Table 1.…”

Section: Micellementioning

confidence: 99%

Recent Advances in Nanotechnology-Based Targeted Delivery Systems of Active Constituents in Natural Medicines for Cancer Treatment

Hu,

Song,

Feng

et al. 2023

Molecules

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Owing to high efficacy and safety, natural medicines have found their way into the field of cancer therapy over the past few decades. However, the effective ingredients of natural medicines have shortcomings of poor solubility and low bioavailability. Nanoparticles can not only solve the problems above but also have outstanding targeting ability. Targeting preparations can be classified into three levels, which are target tissues, cells, and organelles. On the premise of clarifying the therapeutic purpose of drugs, one or more targeting methods can be selected to achieve more accurate drug delivery and consequently to improve the anti-tumor effects of drugs and reduce toxicity and side effects. The aim of this review is to summarize the research status of natural medicines’ nano-preparations in tumor-targeting therapies to provide some references for further accurate and effective cancer treatments.

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Melanoma-targeted photodynamic therapy based on hypericin-loaded multifunctional P123-spermine/folate micelles

Cited by 5 publications

References 62 publications

Design of Liquid Formulation Based on F127-Loaded Natural Dimeric Flavonoids as a New Perspective Treatment for Leishmaniasis

Design of Liquid Formulation Based on F127-Loaded Natural Dimeric Flavonoids as a New Perspective Treatment for Leishmaniasis

Hypericin-loaded in modified theranostic liposomes nanoplatform: a preliminary in vivo study of targeting and diagnosis

Recent Advances in Nanotechnology-Based Targeted Delivery Systems of Active Constituents in Natural Medicines for Cancer Treatment

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