Melanoma detection by enzyme-radioimmunoassay of L-dopa, dopamine, and 3-O-methyldopamine in urine.

Faraj, Bahjat A.; Lawson, David H.; Nixon, Daniel W.; Murray, Douglas R.; Camp, Vernon M.; Ali, Farouk M.; Black, Marcus L.; Stacciarini, W M; Tarcan, Yavuz A.

doi:10.1093/clinchem/27.1.108

Cited by 20 publications

(4 citation statements)

References 18 publications

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“…A standard addition method determined the concentration of NE and DA in urine to be 0.34 μM ± 0.06 ( n = 3) and 0.94 μM ± 0.03 ( n = 3), respectively. These results are in agreement with the reported values . The feasibility of the proposed method was also validated by determining the content of catecholamine in P. oleracea L. leaves.…”

Section: Resultssupporting

confidence: 90%

Selective enrichment of catecholamines using iron oxide nanoparticles followed by CE with UV detection

Lin

Tseng

2012

Electrophoresis

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This study examines the use of unmodified magnetite nanoparticles (Fe(3)O(4) NPs) for selective extraction and enrichment of the catecholamines dopamine (DA), noradrenaline (NE), and adrenaline (E), prior to analysis using capillary electrophoresis with UV detection. Coordination between Fe(3+) on-the-surface Fe(3)O(4) NPs and the catechol moiety of catecholamines enables Fe(3)O(4) NPs to capture catecholamines from an aqueous solution. We obtained maximum loading of catecholamines on the NP surface by adjusting the pH of the solution to 7.0. In addition, catecholamine loading on the Fe(3)O(4) NPs increased in conjunction with NP concentrations. H(3)PO(4) was found to be efficient for the removal of adsorbed catecholamines on the NP surface. Adding 1.2% poly(diallyldimethylammonium chloride) to the background electrolyte resulted in a baseline separation of the liberated catecholamines within 20 min. Under optimal extraction and separation conditions, the limit of detections at a S/N ratio of 3 for E, NE, and DA were 9, 8, and 10 nM, respectively. Significantly, the combination of a phenylboronate-containing spin column and the proposed method was successfully applied to the determination of NE and DA in human urine and NE in Portulaca oleracea L. leaves.

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Section: Resultssupporting

confidence: 90%

Selective enrichment of catecholamines using iron oxide nanoparticles followed by CE with UV detection

Lin

Tseng

2012

Electrophoresis

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“…Patients with metastatic melanoma in particular may have elevated urinary dopamine levels, most likely because of increased conversion of tyrosine to the dopamine precursor L-dopa by tyrosinase in melanoma tumor cells. 21 Although MIBG imaging might be considered in the evaluation of such patients, results from MIBG imaging must be interpreted with caution in patients with possible adrenal metastases from tumors of neural crest origin, such as melanoma, because these tumors may accumulate MIBG. 22 An appropriate treatment strategy for these patients may include preoperative adrenergic blockade, even if the clinical scenario suggests that the likelihood of pheochromocytoma is relatively low.…”

Section: Discussionmentioning

confidence: 99%

Evaluation and surgical resection of adrenal masses in patients with a history of extra-adrenal malignancy

Lenert¹,

Barnett²,

Kudelka³

et al. 2001

Surgery

142

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“…Unfortunately, neither elevated urinary dopamine nor melanuria are considered sufficiently sensitive or specific to form a useful diagnostic test in malignant melanoma. 4,5 However, an understanding of the link between the pathways for catecholamine metabolism and melanogenesis was a major indicator of the likely diagnosis in this case.…”

Section: Melanuriamentioning

confidence: 89%

Bilateral Adrenal Masses: Phaeochromocytoma or Melanoma?

Mackenzie¹,

Ashby²,

Donovan

et al. 2006

J R Soc Med

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Adrenal masses vary in importance from being incidental findings of no clinical significance to having sinister consequences through malignancy. The importance in integrating the clinical history, biochemical findings and results of modern imaging techniques is illustrated in this case. CASE HISTORYA man of 70 presented to his general practitioner with a 2month history of weight loss, anorexia, nausea and abdominal pains. Past medical history included pharyngeal pouch and excision of a malignant melanoma from his left shoulder 7 years previously. The previous melanoma was a 1 cm maximum diameter superficial spreading type of malignant melanoma showing invasion of the epidermis and superficial dermis with a maximum depth of 0.6 mm. The resection margins were clear. The patient had shown no clinical evidence of local recurrence or axillary lymphadenopathy at 6-monthly reviews and was discharged after 5 years of follow-up according to the prevalent protocol.Physical examination was unremarkable except for a scar on his shoulder. Routine blood tests and chest radiograph were normal. He was referred to his local hospital where he was seen a month later and was found to be hypertensive, with a blood pressure of 190/96 mmHg, for which he was commenced on lisinopril. Abdominal ultrasound and subsequent computerized tomography scanning (Figure 1) revealed enlargement of both adrenal glands, reported as 'probably benign'. In view of the hypertension and adrenal masses, urinary catecholamines were measured. 24-hour urinary noradrenaline (254 nmol/24 h) and adrenaline (21 nmol/24 h) excretion were normal, but 24-hour urinary dopamine was grossly elevated at 18 783 nmol (normal 53194 nmol). He was referred to the tertiary centre (3 months after his initial presentation to his general practitioner) for further investigation of possible bilateral dopamine secreting phaeochromocytoma.He was seen the next day. By now he was generally unwell with nausea and vomiting, and had lost 20 kg in weight since the start of his illness. He had no palpitations, sweats or fainting. He looked cachectic and tanned. There was no palpable lymphadenopathy. Blood pressure was 159/85 mmHg. Large masses were palpable in both abdominal flanks. Adrenal insufficiency was suspected and after performance of a short Synacthen test (baseline cortisol 296 nmol/L, 30-min cortisol 268 nmol/L) he was started on hydrocortisone. His symptoms improved dramatically with increases in appetite and weight, and rapid resolution of the nausea and vomiting.After correction of his hypoadrenalism, routine blood tests were normal. Plasma noradrenaline (2.79 nmol/L; normal range 0.59-5.33 nmol/L) and adrenaline (50.055 nmol/L; normal range 50.82 nmol/L) were not elevated, 153 Figure 2 Melanuria-a specimen of the patient's urine that had been left standing. The dark colour is due to the oxidation of melanogens in the urine.

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Melanoma detection by enzyme-radioimmunoassay of L-dopa, dopamine, and 3-O-methyldopamine in urine.

Cited by 20 publications

References 18 publications

Selective enrichment of catecholamines using iron oxide nanoparticles followed by CE with UV detection

Selective enrichment of catecholamines using iron oxide nanoparticles followed by CE with UV detection

Evaluation and surgical resection of adrenal masses in patients with a history of extra-adrenal malignancy

Bilateral Adrenal Masses: Phaeochromocytoma or Melanoma?

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