Melanocyte stimulating hormone peptides inhibit TNF-α signaling in human dermal fibroblast cells

Hill, Rebecca P.; MacNeil, Sheila; Haycock, John W.

doi:10.1016/j.peptides.2005.03.061

Cited by 37 publications

(33 citation statements)

References 37 publications

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“…One such antimicrobial peptide is alpha-melanocyte-stimulating hormone (␣-MSH), an ancient linear tridecapeptide that is well recognized for its endogenous anti-inflammatory and melanogenic properties (8,15,29,36). The peptide is a cleavage product of pro-opiomelanocortic precursor (POMC), which is expressed in the pituitary gland and in other cells such as neutrophils, monocytes, melanocytes, fibroblasts, and keratinocytes (9,23,24). The sequence of this neuropeptide is Ac-Ser-Tyr-Ser-Met-Glu-His-Phe-Arg-TrpGly-Lys-Pro-Val-NH 2 .…”

mentioning

confidence: 99%

“…The sequence of this neuropeptide is Ac-Ser-Tyr-Ser-Met-Glu-His-Phe-Arg-TrpGly-Lys-Pro-Val-NH 2 . It controls inflammation and high temperature through immunomodulation, downregulates several proinflammatory cytokines, and stimulates production of antiinflammatory cytokines such as interleukin-10 (IL-10) (2,14,23). The widespread distribution of this peptide and its receptor in many barrier cells such as keratinocytes and in various immune cells suggests its potential role in host defense.…”

mentioning

confidence: 99%

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C-Terminal Amino Acids of Alpha-Melanocyte-Stimulating Hormone Are Requisite for Its Antibacterial Activity against Staphylococcus aureus

Singh

Mukhopadhyay

2011

Antimicrob Agents Chemother

105

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Alpha-melanocyte-stimulating hormone (␣-MSH) is an endogenous neuropeptide that is known for its anti-inflammatory and antipyretic activities.We recently demonstrated that ␣-MSH possesses staphylocidal activity and causes bacterial membrane damage. To understand the role of its amino acid sequences in the staphylocidal mechanism, in the present study we investigated the antimicrobial activities of different fragments of ␣-MSH, i.e., ␣-MSH(6-13), ␣-MSH(11-13), and ␣-MSH(1-5), and compared them with that of the entire peptide. Our results showed that peptides containing the C-terminal region of ␣-MSH, namely, ␣-MSH(6-13) and ␣-MSH(11-13), efficiently killed >90% of both methicillin-sensitive and -resistant Staphylococcus aureus cells in the micromolar range and ϳ50% of these cells in the nanomolar range; their efficiency was comparable to that of the entire ␣-MSH, whereas the peptide containing the N-terminal region, ␣-MSH(1-5), was found to be ineffective against S. aureus. The antimicrobial activity of ␣-MSH and its C-terminal fragments was not affected by the presence of NaCl or even divalent cations such as Ca 2؉ and Mg 2؉ . Similar to the case for the parent peptide, ␣-MSH(6-13) and ␣-MSH(11-13) also depolarized and permeabilized Staphylococcus cells (ϳ70 to 80% of the cells were depolarized and lysed after 2 h of peptide exposure at micromolar concentrations). Furthermore, scanning and transmission electron microscopy showed remarkable morphological and ultrastructural changes on S. aureus cell surface due to exposure to ␣-MSH-based peptides. Thus, our observations indicate that C-terminal fragments of ␣-MSH retain the antimicrobial activity of entire peptide and that their mechanism of action is similar to that of full-length peptide. These observations are important and are critical in the rational design of ␣-MSH-based therapeutics with optimal efficacy.

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confidence: 99%

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confidence: 99%

C-Terminal Amino Acids of Alpha-Melanocyte-Stimulating Hormone Are Requisite for Its Antibacterial Activity against Staphylococcus aureus

Singh

Mukhopadhyay

2011

Antimicrob Agents Chemother

105

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“…Since IL-10 reduces the production of pro-inflammatory cytokines in macrophages, its upregulation could result in anti-inflammatory influences. Studies on different cell types of human skin, including pigment cells, fibroblastic cells and dermal microvascular endothelial cells, as well as rat mast cells, showed that -MSH is able to suppress the expression of several intracellular adhesion molecules (interstitial adhesion molecule-1 -ICAM-1 and P-selectin) induced by proinflammatory stimuli such as IFN-, LPS and TNF-Böhm et al, 2005;Hill et al, 2006;Sarkar et al, 2003). Finally, the inhibition of adhesion and transmigration of inflammatory cells may contribute to the anti-inflammatory potential of -MSH (Böhm et al, 2005;Scholzen et al, 2003).…”

Section: The Place and Importance Of -Msh And Met-enkephalin In Immumentioning

confidence: 99%

Enkorten – A Potential Drug for the Treatment of Rheumatoid Arthritis

Mulabegović¹,

Kusturica²,

Todić-Rakanović³

et al. 2012

Rheumatoid Arthritis - Treatment

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“…With the development of non-invasive transdermal delivery methods [10], topical peptides have become new options for the treatment of skin problems. In the literature, several types of peptides have been reported to have anti-inflammatory effects [1,2,4,6,[11][12][13]. However, the effects of short chain peptides have not been thoroughly studied, even though better absorption is anticipated.…”

Section: Introductionmentioning

confidence: 99%