Melanocyte–keratinocyte interaction induces calcium signalling and melanin transfer to keratinocytes

Joshi, Preeti G.; Nair, Nirmala; Begum, Gulnaz; Joshi, Nanda B.; Sinkar, Vilas P.; Vora, Shilpa

doi:10.1111/j.1600-0749.2007.00397.x

Cited by 81 publications

(58 citation statements)

References 24 publications

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“…Melanin which accumulated inside cells were highlighted by Fontana-Masson staining (Joshi et al, 2007). Cells were fi xed in 4% pformaldehyde for 10 min at room temperature and stained for melanin by using a Fontana-Masson staining kit from American Master*Tech Scientifi c, Inc. (Lodi, CA, USA), according to the manufacturer's instructions.…”

Section: Methodsmentioning

confidence: 99%

p‐coumaric acid not only inhibits human tyrosinase activity in vitro but also melanogenesis in cells exposed to UVB

Koh²,

Boo

2010

Phytotherapy Research

122

102

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Tyrosinase (TYR) catalyzes rate-limiting steps of melanogenesis and thus its inhibitors are potentially useful as hypopigmenting agents. Recently, p-coumaric acid (p-CA) has been suggested to interfere with the pro-melanogenic actions of tyrosine due to its structural similarity with tyrosine (An SM et al., Br J Dermatol 2008. 159: 292). In this study, we compared the inhibitory effects of p-CA and two other well known TYR inhibitors used in cosmetics--arbutin and kojic acid--on the catalytic activities of mushroom, murine and human TYRs in vitro, using tyrosine and 3,4-dihydroxyphenylalanine (DOPA) as substrates. The results showed that p-CA is a weaker inhibitor of mushroom TYR but much stronger inhibitor of human or murine TYR in comparison with kojic acid and arbutin. In addition, p-CA inhibited human TYR at much lower concentrations than those required for the inhibition of murine or mushroom TYRs. Enzyme kinetics analysis indicated that p-CA is a mixed type (for tyrosine) or competitive inhibitor (for DOPA) of human TYR. Potent antimelanogenic effects of p-CA were observed in human epidermal melanocytes exposed to UVB. The present study demonstrated that p-CA is a potent and selective inhibitor of human TYR and is potentially useful as a hypopigmenting agent.

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Section: Methodsmentioning

confidence: 99%

p‐coumaric acid not only inhibits human tyrosinase activity in vitro but also melanogenesis in cells exposed to UVB

Koh²,

Boo

2010

Phytotherapy Research

122

102

View full text Add to dashboard Cite

show abstract

“…Melanin which accumulated inside cells was highlighted by Fontana-Masson staining [15,19]. Cell morphology and pigmentation were examined under a phase-contrast microscope (Eclipse TS100, Nikon Instruments Inc., Melville, NY, USA).…”

Section: Fontana-masson Stainingmentioning

confidence: 99%

Comparison of the antimelanogenic effects of p-coumaric acid and its methyl ester and their skin permeabilities

Song

Kim

et al. 2011

Journal of Dermatological Science

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“…mature melanosomes to neighboring keratinocytes, resulting in visible skin pigmentation (3) and protecting the keratinocytes from the deleterious effects of UV light (4). Therefore, melanocytes play an important role in keratinocyte functions.…”

mentioning

confidence: 99%

Keratinocyte-derived Laminin-332 Promotes Adhesion and Migration in Melanocytes and Melanoma

Chung

Suh

Han

et al. 2011

Journal of Biological Chemistry

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Melanocytes are highly motile cells that play an integral role in basic skin physiological processes such as wound healing and proper skin pigmentation. It has been postulated that surrounding keratinocytes contribute to melanocyte migration, but underlying mechanisms remain rather vague so far. In this study, we set out to analyze the specific potential contribution of keratinocyte components to melanocytes and melanoma cell migration-related processes. Our studies revealed that A375 human melanoma cell attachment, spreading, and migration are interestingly better supported by HaCaT keratinocyte extracellular matrix (ECM) than by self-derived A375 ECM. Moreover, HaCaT ECM caused increased integrin ␣6 expression, adhesion-mediated focal adhesion kinase phosphorylation, and focal adhesion formations. Similar effects were confirmed in human melanocytes. Furthermore, we found that keratinocyte-derived soluble factors did not appear to significantly contribute to these processes. Specific extrinsic factors that promoted melanoma migration were attributed to keratinocyte-derived laminin-332, whereas alternative ECM component such as laminin-111 and fibronectin functions appeared to have insignificant contributions. Taken together, these studies implicate extrinsic laminin-332 in promoting the high mobility property and perhaps invasiveness inherently characteristic of, and that are the menace of, melanocytes and melanomas, respectively.Melanocytes, which are present in the skin, hair, eyes, and ears, synthesize melanin via a process known as melanogenesis (1), and thus play a key role in the pigmentary system of the skin in the body. These cells are located in the bottom layer of the skin (basal epidermis), where they comprise 5-10% of the total cells. The major cells comprising the epidermis are keratinocytes, which are organized into the basal cell layer, spinous cell layer, granular cell layer, and keratinized squames (2). The keratinocytes in the basal cell layer gradually differentiate, proliferate, and migrate upward to form the primary protection of the body from the outside environment.There is a close and important functional association between melanocytes and keratinocytes. Melanocytes transfer mature melanosomes to neighboring keratinocytes, resulting in visible skin pigmentation (3) and protecting the keratinocytes from the deleterious effects of UV light (4). Therefore, melanocytes play an important role in keratinocyte functions. Reciprocally, keratinocytes mediate melanocyte functions via several pathways, including cell-cell adhesion, cell-matrix adhesion, and paracrine signaling (5). Normal melanocytes maintain cellcell adhesion with keratinocytes by expressing cell-cell adhesion proteins such as E-cadherin, desmoglein 1, and connexins (6). In turn, keratinocytes secrete many paracrine factors to melanocytes, including ␣-melanocyte-stimulating hormone (␣-MSH), 2 adrenocorticotrophic hormone, endothelin-1, -2, and -3, FGF-2, and hepatocyte growth factor, all of which regulate the proliferation and di...

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Melanocyte–keratinocyte interaction induces calcium signalling and melanin transfer to keratinocytes

Cited by 81 publications

References 24 publications

p‐coumaric acid not only inhibits human tyrosinase activity in vitro but also melanogenesis in cells exposed to UVB

p‐coumaric acid not only inhibits human tyrosinase activity in vitro but also melanogenesis in cells exposed to UVB

Comparison of the antimelanogenic effects of p-coumaric acid and its methyl ester and their skin permeabilities

Keratinocyte-derived Laminin-332 Promotes Adhesion and Migration in Melanocytes and Melanoma

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