Melanocyte Growth Factor in Normal Human Skin

Horikawa, Tsuyoshi; Nishino, Keisuke; Mishima, Yutaka

doi:10.1111/j.1600-0749.1991.tb00313.x

Cited by 10 publications

(6 citation statements)

References 16 publications

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“…4). TPA stimulates the growth of normal melanocytes at this concentration (Horikawa et al, 1991). The growth of HMSKO, MeWo, and HMV-I cells was inhibited by approximately 10-30% at 48-72 h after the addition of TPA.…”

Section: Resultsmentioning

confidence: 97%

“…Normal melanocytes were isolated from infantile foreskins as described previously (Horikawa et al, 1991) and maintained in PC-1 chemically defined medium (Ventrex Bio Ventures Group, Portland, ME) containing 200 U/ml penicillin, 100 pg/ml streptomycin, 2 ng/ml human recombinant basic fibroblast growth factor (bFGF) (Boehringer Mannheim-Yamanouchi Co. Ltd., Tokyo, Japan), and 0.5 mM dibutyryl cyclic AMP (Sigma Chemical Co., St. Louis, MO). Four melanoma cell lines were used in this study.…”

Section: Cells and Cell Culturementioning

confidence: 99%

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Deletion of specific protein kinase C subspecies in human melanoma cells

et al. 1996

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It has been shown that tumor-promoting phorbol ester, 12-O-tetradecanoylphorbol-13-acetate (TPA), stimulates the proliferation of normal human melanocytes, whereas it inhibits the growth of human melanoma cell lines. The expression of protein kinase C (PKC) subspecies, the major intracellular receptors for TPA, was examined in normal melanocytes and the four melanoma cell lines HM3KO, MeWo, HMV-1, and G361. PKC was partially purified and then separated into subspecies by column chromatography on Mono Q and hydroxyapatite successively, and finally subjected to immunoblot analysis using antibodies specific for the PKC subspecies. Of the PKC subspecies examined, delta-, epsilon-, and zeta-PKC were detected in both normal melanocytes and the four melanoma cell lines. In contrast, both alpha-PKC and beta-PKC were expressed in normal melanocytes, whereas either alpha-PKC or beta-PKC was detected in melanoma cells. Specifically, HM3KO, MeWo, and HMV-1 cells were shown to contain alpha-PKC but not beta-PKC, while G361 cells expressed beta-PKC but not alpha-PKC. The growth of these melanoma cells was suppressed by TPA treatment, and the growth of the G361 cells lacking alpha-PKC was inhibited more efficiently than the other melanoma cell lines which lacked beta-PKC. It was further shown that beta-PKC was not detected in freshly isolated human primary or metastatic melanoma tissues. These results suggest that the expression of alpha-PKC or beta-PKC may be altered during the malignant transformation of normal melanocytes and that loss of alpha-PKC or beta-PKC may be related to the inhibitory effect of TPA on the growth of melanoma cells.

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Section: Resultsmentioning

confidence: 97%

Section: Cells and Cell Culturementioning

confidence: 99%

Deletion of specific protein kinase C subspecies in human melanoma cells

et al. 1996

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“…Melanocytes were cultured as described previously. 15 Briefly, an epidermal suspension of a skin sample from the HPS patient was obtained by dispase and, following trypsin treatment, was cultured in Ham's F10 medium supplemented with 10% fetal bovine serum, 5 mg mL 21 insulin, isobutylmethylxanthine, 12-O-tetradecanoylphorbol-13-acetate and pituitary extract at 37 8C with 5% CO 2 . Medium was changed twice a week, and contaminating fibroblasts were eliminated by geneticin treatment.…”

Section: Cell Culturementioning

confidence: 99%

Heterozygous HPS1 mutations in a case of Hermansky-Pudlak syndrome with giant melanosomes

Horikawa¹,

Araki²,

Fukai³

et al. 2000

Br J Dermatol

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We report a Japanese man with Hermansky±Pudlak syndrome, having oculocutaneous albinism with a bleeding diathesis. Gene analysis of the patient's peripheral blood cells revealed that he was a compound heterozygote for HPS1 gene mutations. One of the mutations was a novel frameshift mutation at codon 321 (a G insertion) in exon 11 (<962±963insG), and the other was a 5 H splicejunction mutation of IVS5 (IVS5 1 5G3A). The content of eumelanin in the patient's hairs was significantly reduced. Histological analysis using light and electron microscopy revealed that melanocytes in the patient's epidermis contained an appreciable number of giant melanosomes. Cultured melanocytes from the patient's skin also contained giant melanosomes. Our finding of mutations in the HPS1 gene in relation to abnormalities in melanosome morphology and melanin production shed light on the role and function of the HPS1 gene product in the synthesis of melanosomes and melanin pigment.

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“…Melanocytes were cultured as described previously 15 . Briefly, an epidermal suspension of a skin sample from the HPS patient was obtained by dispase and, following trypsin treatment, was cultured in Ham's F10 medium supplemented with 10% fetal bovine serum, 5 µg mL −1 insulin, isobutylmethylxanthine, 12‐ O ‐tetradecanoyl‐phorbol‐13‐acetate and pituitary extract at 37 °C with 5% CO 2 .…”

Section: Methodsmentioning

confidence: 99%

HeterozygousHPS1mutations in a case of Hermansky-Pudlak syndrome with giant melanosomes

Horikawa

Araki

Fukai

et al. 2000

British Journal of Dermatology

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We report a Japanese man with Hermansky-Pudlak syndrome, having oculocutaneous albinism with a bleeding diathesis. Gene analysis of the patient's peripheral blood cells revealed that he was a compound heterozygote for HPS1 gene mutations. One of the mutations was a novel frameshift mutation at codon 321 (a G insertion) in exon 11 ( approximately 962-963insG), and the other was a 5' splice-junction mutation of IVS5 (IVS5 + 5G-->A). The content of eumelanin in the patient's hairs was significantly reduced. Histological analysis using light and electron microscopy revealed that melanocytes in the patient's epidermis contained an appreciable number of giant melanosomes. Cultured melanocytes from the patient's skin also contained giant melanosomes. Our finding of mutations in the HPS1 gene in relation to abnormalities in melanosome morphology and melanin production shed light on the role and function of the HPS1 gene product in the synthesis of melanosomes and melanin pigment.

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Melanocyte Growth Factor in Normal Human Skin

Cited by 10 publications

References 16 publications

Deletion of specific protein kinase C subspecies in human melanoma cells

Deletion of specific protein kinase C subspecies in human melanoma cells

Heterozygous HPS1 mutations in a case of Hermansky-Pudlak syndrome with giant melanosomes

HeterozygousHPS1mutations in a case of Hermansky-Pudlak syndrome with giant melanosomes

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