Melanin production by tyrosinase activity on a tyrosine-rich peptide fragment and pH-dependent self-assembly of its lipidated analogue

Hutchinson, Jessica; Hamley, Ian W.; Edwards-Gayle, Charlotte J. C.; Castelletto, Valeria; Piras, Cristian; Cramer, Rainer; Kowalczyk, Radosław M.; Seitsonen, Jani; Ruokolainen, Janne; Rambo, Robert P.

doi:10.1039/c9ob00550a

Cited by 14 publications

(6 citation statements)

References 39 publications

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“…Data are presented as a function of q = 4π sin θ /λ, where 2θ is the scattering angle. Membranes were mounted in a custom-designed enclosed gel cell holder 18 for gels and pastes. All measurements were performed at 20 o C. Membrane Assembly.…”

Section: Methodsmentioning

confidence: 99%

Magnetic Field-Induced Alignment of Nanofibrous Supramolecular Membranes: A Molecular Design Approach to Create Tissue-like Biomaterials

Radvar

Shi

Grasso

et al. 2020

ACS Appl. Mater. Interfaces

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A molecular design approach to fabricate nanofibrous membranes by self-assembly of aromatic cationic peptides with hyaluronic acid (HA) and nanofiber alignment under a magnetic field is reported. Peptides are designed to contain a block composed of four phenylalanine residues at the C-terminus, to drive their self-assembly by hydrophobic association and aromatic stacking, and a positively charged domain of lysine residues for electrostatic interaction with HA. These two blocks are connected by a linker with a variable number of amino acids and ability to adopt distinct conformations. Zeta potential measurements and circular dichroism confirm their positive charge and variable conformation (random coil, -sheet or -helix), which depend on the pH and sequence. Their self-assembly, examined by fluorescence spectroscopy, small-angle X-ray scattering and transmission electron microscopy, show the formation of fiber-like nanostructures in the micromolar range. When the peptides are combined with HA, hydrogels or flat membranes are formed. The molecular structure tunes the mechanical behavior of the membranes and the nanofibers align in the direction of magnetic field due to the high diamagnetic anisotropy of phenylalanine residues. Mesenchymal stem cells cultured on magnetically-aligned membranes elongate in direction of the nanofibers supporting their application for soft tissue engineering.

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Section: Methodsmentioning

confidence: 99%

Magnetic Field-Induced Alignment of Nanofibrous Supramolecular Membranes: A Molecular Design Approach to Create Tissue-like Biomaterials

Radvar

Shi

Grasso

et al. 2020

ACS Appl. Mater. Interfaces

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“…Es la enzima responsable de la biosíntesis de melanina a partir de la tirosina; la melanina es el pigmento que confiere el color negro al pelo, la fibra, la lana. La falta de cobre conducirá a trastornos de la biosíntesis de melanina (Hutchinson et al, 2019).…”

Section: Tirosinasaunclassified

Nutrición animal: Texto de formación universitaria

Huanca¹

2023

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El texto que se pone a consideración del lector, en su edición digital, revisada y corregida, ha sido preparado con fines académicos, para la formación de médicos veterinarios y/o zootecnistas, en el contexto del Altiplano de Puno-Perú; sin embargo, puede servir como referencia para todo lector interesado en la nutrición animal. Incluye seis unidades temáticas: fisiología digestiva de los animales, como uno de los procesos nutricionales clave, seguida de energía (carbohidratos), lípidos, proteínas, minerales y vitaminas, y una unidad temática adicional sobre la evaluación de la composición química y calorimetría de los alimentos.

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“…However, the parent peptide without the lipid part did not show self-assembly behavior. At neutral pH and high concentration, lipidated heptapeptide turns into hydrogel, while at different pH systems it shows different aggregated structures like fibrils, micelles, and nanosheets (Hutchinson et al, 2019).…”

Section: Lipopeptide-based Nanohydrogel In Brain Drug Deliverymentioning

confidence: 99%

Protein-Based Nanohydrogels for Bioactive Delivery

et al. 2021

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Hydrogels possess a unique three-dimensional, cross-linked network of polymers capable of absorbing large amounts of water and biological fluids without dissolving. Nanohydrogels (NGs) or nanogels are composed of diverse types of polymers of synthetic or natural origin. Their combination is bound by a chemical covalent bond or is physically cross-linked with non-covalent bonds like electrostatic interactions, hydrophobic interactions, and hydrogen bonding. Its remarkable ability to absorb water or other fluids is mainly attributed to hydrophilic groups like hydroxyl, amide, and sulphate, etc. Natural biomolecules such as protein- or peptide-based nanohydrogels are an important category of hydrogels which possess high biocompatibility and metabolic degradability. The preparation of protein nanohydrogels and the subsequent encapsulation process generally involve use of environment friendly solvents and can be fabricated using different proteins, such as fibroins, albumin, collagen, elastin, gelatin, and lipoprotein, etc. involving emulsion, electrospray, and desolvation methods to name a few. Nanohydrogels are excellent biomaterials with broad applications in the areas of regenerative medicine, tissue engineering, and drug delivery due to certain advantages like biodegradability, biocompatibility, tunable mechanical strength, molecular binding abilities, and customizable responses to certain stimuli like ionic concentration, pH, and temperature. The present review aims to provide an insightful analysis of protein/peptide nanohydrogels including their preparation, biophysiochemical aspects, and applications in diverse disciplines like in drug delivery, immunotherapy, intracellular delivery, nutraceutical delivery, cell adhesion, and wound dressing. Naturally occurring structural proteins that are being explored in protein nanohydrogels, along with their unique properties, are also discussed briefly. Further, the review also covers the advantages, limitations, overview of clinical potential, toxicity aspects, stability issues, and future perspectives of protein nanohydrogels.

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Melanin production by tyrosinase activity on a tyrosine-rich peptide fragment and pH-dependent self-assembly of its lipidated analogue

Abstract: The self-assembly of a palmitoylated peptide C16-EELNRYY based on a fragment of the gut hormone peptide PYY3–36 is investigated.

Cited by 14 publications

References 39 publications

Magnetic Field-Induced Alignment of Nanofibrous Supramolecular Membranes: A Molecular Design Approach to Create Tissue-like Biomaterials

Magnetic Field-Induced Alignment of Nanofibrous Supramolecular Membranes: A Molecular Design Approach to Create Tissue-like Biomaterials

Nutrición animal: Texto de formación universitaria

Protein-Based Nanohydrogels for Bioactive Delivery

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