Melanin-concentrating hormone receptor-1 is located in primary cilia of the dorsal raphe neurons

Ciliary melanin‐concentrating hormone receptor 1 (MCHR1) is widely distributed in the murine CNS in a sex‐independent manner

“…We also reproduced the ciliary labeling observed in the dorsal raphe of rats by Niño‐Rivero et al. (2019) and in the olfactory bulb, nucleus accumbens, hippocampus, and hypothalamus observed by Berbari, Lewis, et al. (2008).…”

“…The other two published works that employed immunohistochemistry for MCHR1 were Berbari, Johnson, et al. (2008) and Niño‐Rivero, Torterolo, and Lagos (2019), both using a antibody now discontinued to demonstrate the presence of MCHR1 in the neuronal primary cilia of specific areas within the CNS of mice and rats. We demonstrated in this work that antibody PA5‐24182 is a suitable replacement for previous antibodies, generating negative results in antibody omission tests, not labeling cilia in tissue from two strains of Mchr1 −/− mice , resulting in a pattern of MCHR1 distribution that mirrors that of Mchr1 mRNA in several key areas, and specifically labeling MCHR1 in neuronal primary cilia , as demonstrated by the colocalization with AC3.…”

The distribution of neuronal primary cilia immunoreactive to melanin-concentrating hormone receptor 1 (MCHR1) in the murine prosencephalon

“…It is unclear, at this moment, if this results from all MCHR1 being found solely at primary cilia, or if the antibody targets ciliary MCHR1 selectively, either due to slight post-translational differences between ciliary and non-ciliary MCHR1 or due to facilitated antibody access to its epitope in the cilium as compared to the membrane or synaptic space. Hervieu et al (2000) found labeling with anti-MCHR1 to be confined to the plasma membrane, indicating that the antibody used in that work targets specifically membrane MCHR1, while both Berbari et al (2008) and Niño-Rivero et al (2019) found labeling to be exclusively ciliary. Regardless of the case, our work demonstrates that MCHR1 presence in the cilium is widespread, what has important functional implications.…”

“…Hervieu et al (2000) were the first to use antibodies to map MCHR1, but there is no mention as to the subcellular localization of the labeling obtained by those authors. The other two published works that employed immunohistochemistry for MCHR1 were Berbari et al (2008) and Niño-Rivero et al (2019), who used a now discontinued antibody to demonstrate the presence of MCHR1 in the neuronal primary cilia of specific areas within the CNS of mice and rats. We demonstrated in this work that antibody PA5-24182 is a suitable replacement for the labeling of MCHR1, generating negative results in antibody omission tests, not labeling tissue from Mchr1 -/-, and specifically labeling MCHR1 in neuronal primary cilia, as demonstrated by the colocalization with AC3.…”

Morfologia comparada do sistema do hormônio concentrador de melanina.

“…Gao and van den Pol (17) suggest that MCHR1 is found in the presynaptic membrane, based on the property of MCH to decrease the frequency of miniature glutamate-mediated postsynaptic currents in the presence of tetrodotoxin. Berbari et al 60, on the other hand, were the first to find MCHR1 in the neuronal primary cilia of transfected cultured cells and slices of mice brains, observation replicated in rats using the same antibody (61) . The neuronal primary cilium is a sensory structure, a single non-motile cilium that extends from the membrane towards the extracellular space (ECS) and is often coated in receptors (62).…”