Melampsora rust species on biomass willows in central and north-eastern Germany

Bubner, Ben; Wunder, Sebastian; Zaspel, I.; Zander, Matthias; Gloger, Jan; Fehrenz, Steffen; Ulrichs, Christian

doi:10.1016/j.funbio.2014.08.002

Cited by 10 publications

(1 citation statement)

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“…The rate of successful ITS sequencing (63%) is relatively low. In a previous study on Melampsora rust fungi on Salix , the rate of ITS sequencing success (93%) was much higher (Bubner et al 2014). The Melampsora study comprised exclusively freshly collected specimens not older than half a year, whereas we also analysed herbarium material that was several years old.…”

Section: Discussionmentioning

confidence: 90%

Species identification of European forest pathogens of the genus Milesina (Pucciniales) using urediniospore morphology and molecular barcoding including M. woodwardiana sp. nov.

Bubner

Buchheit

Friedrich

et al. 2019

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Species of rust fungi of the genus Milesina (Pucciniastraceae, Pucciniales) are distributed mainly in northern temperate regions. They host-alternate between needles of fir (Abies spp.) and fronds of ferns (species of Polypodiales). Milesina species are distinguished based on host taxonomy and urediniospore morphology. In this study, 12 species of Milesina from Europe were revised. Specimens were examined by light and scanning electron microscopy for urediniospore morphology with a focus on visualising germ pores (number, size and position) and echinulation. In addition, barcode loci (ITS, nad6, 28S) were used for species delimitation and for molecular phylogenetic analyses. Barcodes of 72 Milesina specimens were provided, including 11 of the 12 species. Whereas urediniospore morphology features were sufficient to distinguish all 12 Milesina species except for 2 (M.blechni and M.kriegeriana), ITS sequences separated only 4 of 11 species. Sequencing with 28S and nad6 did not improve species resolution. Phylogenetic analysis, however, revealed four phylogenetic groups within Milesina that also correlate with specific urediniospore characters (germ pore number and position and echinulation). These groups are proposed as new sections within Milesina (sections Milesina, Vogesiacae M. Scholler & Bubner, sect. nov., Scolopendriorum M. Scholler & Bubner, sect. nov. and Carpaticae M. Scholler & Bubner, sect. nov.). In addition, Milesinawoodwardiana Buchheit & M. Scholler, sp. nov. on Woodwardiaradicans, a member of the type section Milesina, is newly described. An identification key for European Milesina species, based on urediniospore features, is provided.

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