Melamine-Induced Urolithiasis in a Drosophila Model

Chen, Wen Chi; Lin, Wei-Yong; Chen, Huey Yi; Chang, Chiao Hui; Tsai, Fuu Jen; Man, Kee Ming; Shen, Jui-Lung; Chen, Yung‐Hsiang

doi:10.1021/jf204647p

Cited by 16 publications

(18 citation statements)

References 23 publications

(35 reference statements)

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“…Attempted mitigation of the effects of melamine with potassium-citrate resulted in a reversal of the effects on D. melanogaster lifespan, but no significant effect on Malpighian tubule crystal formation. 24 As with other Drosophila models of environmentally induced nephrolithiasis, the melamine-induced nephrolithiasis Drosophila model has limited applicability to the formation of idiopathic calcium oxalate nephrolithiasis in humans, as the crystals are neither calcium oxalate nor uric acid.…”

Section: Drosophila Models Of Human Nephrolithiaismentioning

confidence: 99%

Drosophila melanogaster as an Emerging Translational Model of Human Nephrolithiasis

et al. 2013

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Purpose The limitations imposed by human clinical studies and mammalian models of nephrolithiasis have hampered the development of effective medical treatments and preventative measures for decades. The simple but elegant Drosophila melanogaster is emerging as a powerful translational model of human disease, including nephrolithiasis and may provide important information essential to our understanding of stone formation. We present the current state of research using D. melanogaster as a model of human nephrolithiasis. Materials and Methods A comprehensive review of the English language literature was performed using PUBMED. When necessary, authoritative texts on relevant subtopics were consulted. Results The genetic composition, anatomic structure and physiologic function of Drosophila Malpighian tubules are remarkably similar to those of the human nephron. The direct effects of dietary manipulation, environmental alteration, and genetic variation on stone formation can be observed and quantified in a matter of days. Several Drosophila models of human nephrolithiasis, including genetically linked and environmentally induced stones, have been developed. A model of calcium oxalate stone formation is among the most recent fly models of human nephrolithiasis. Conclusions The ability to readily manipulate and quantify stone formation in D. melanogaster models of human nephrolithiasis presents the urologic community with a unique opportunity to increase our understanding of this enigmatic disease.

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Section: Drosophila Models Of Human Nephrolithiaismentioning

confidence: 99%

Drosophila melanogaster as an Emerging Translational Model of Human Nephrolithiasis

et al. 2013

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“…For example, a test for the effect of melamine on crystal formation in Drosophila was performed. The results indicate that the administration of melamine caused crystal formation in a mixture of CaOx, calcium phosphate, uric acid, and melamine crystals [18]. In a previous study by Ho et al, it was found that potassium citrate could prevent crystal formation in EG-induced CaOx nephrolithiasis in Drosophila.…”

Section: Introductionmentioning

confidence: 99%

An Emerging Translational Model to Screen Potential Medicinal Plants for Nephrolithiasis, an Independent Risk Factor for Chronic Kidney Disease

Shen

Man

et al. 2014

Evidence-Based Complementary and Alternative Medicine

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Pharmacological therapy for urolithiasis using medicinal plants has been increasingly adopted for the prevention of its recurrence. A Drosophila melanogaster model developed for translational research of urolithiasis was applied to evaluate agents with potential antilithic effects and calcium oxalate (CaOx) formation. Potential antilithic herbs were prepared in a mixture of food in a diluted concentration of 5,000 from the original extract with 0.5% ethylene glycol (EG) as the lithogenic agent. The control group was fed with food only. After 3 weeks, flies (n ≥ 150 for each group) were killed using CO2 narcotization, and the Malpighian tubules were dissected, removed, and processed for polarized light microscopy examination of the crystals. The crystal formation rate in the EG group was 100.0%. In the study, 16 tested herbal drugs reached the crystal formation rate of 0.0%, including Salviae miltiorrhizae, Paeonia lactiflora, and Carthami flos. Scutellaria baicalensis enhanced CaOx crystal formation. Two herbal drugs Commiphora molmol and Natrii sulfas caused the death of all flies. Our rapid screening methods provided evidence that some medicinal plants have potential antilithic effects. These useful medicinal plants can be further studied using other animal or human models to verify their effects.

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“…This urolithiasis model was based on our previous well‐established fruit fly model [33–35]. In brief, we used wild‐type male fruit flies, Drosophila melanogaster Canton‐S, as the animal model for our experiments.…”

Section: Methodsmentioning

confidence: 99%

“…We previously developed a novel Drosophila model for the study of nephrolithiasis. The model provides a new, well‐established drug discovery platform for this common disease [33–35]. The flies are easy to breed, making this method a cost‐effective alternative to the typically used rat model [36,37].…”

Section: Introductionmentioning

confidence: 99%

Effects of commercial citrate‐containing juices on urolithiasis in a Drosophila model

Chen

et al. 2013

The Kaohsiung J of Med Scie

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Diet modification plays an important role in nephrolithiasis. Development of an easy, ready-to-use beverage such as a commercial juice drink to use as a preventive treatment for renal calculi formation would be widely welcomed. We previously developed a novel Drosophila model for the study of nephrolithiasis. It provides a new well-established drug discovery platform for this common disease. In our current study, we used the Drosophila model to investigate the preventive effects of various commercial juices as potential treatments for nephrolithiasis. Our results showed that apple, cranberry, orange, and pomegranate juices failed to reduce calcium oxalate (CaOx) crystal formation, whereas our positive control-potassium citrate (K-citrate)-significantly prevented CaOx crystal formation. Unlike the commercial fruit juices that were tested, the administration of K-citrate significantly ameliorated the ethylene glycol (EG)-induced life-span reduction in treated flies. These results indicate that EG-induced CaOx nephrolithiasis in Drosophila can be prevented by K-citrate, but not by commercial citrate-containing juices. However, the inhibitory capability of citrate-containing juices to reduce renal stone formation in humans requires further elucidation.

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Melamine-Induced Urolithiasis in a Drosophila Model

Cited by 16 publications

References 23 publications

Drosophila melanogaster as an Emerging Translational Model of Human Nephrolithiasis

Drosophila melanogaster as an Emerging Translational Model of Human Nephrolithiasis

An Emerging Translational Model to Screen Potential Medicinal Plants for Nephrolithiasis, an Independent Risk Factor for Chronic Kidney Disease

Effects of commercial citrate‐containing juices on urolithiasis in a Drosophila model

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