Meiotic studies in an azoospermic boar carrying a Y;14 translocation

Pinton, Alain; Raymond‐Letron, Isabelle; Berland, HM; Bonnet, N.; Calgaro, Anne; Garnier-Bonnet, Amélie; Yerle, Martine M.; Ducos, Alain

doi:10.1159/000118747

Cited by 27 publications

(41 citation statements)

References 52 publications

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“…More recently, the scope of the SC approach has been greatly improved by the introduction of immunolocalization of specific proteins associated with the main events in meiosis including synapsis [Hart et al, 2008;Pinton et al, 2008]. Our approach, using immunolocalization of synaptonemal-core-specific protein for analyzing the presumed 9/14 reciprocal translocation, not only detected the quadrivalent configuration (SSC9, SSC14 and the derived chromosomes 9 and 14) as expected in a reciprocal translocation involving autosomes, it also showed the X-Y bivalent characteristic of normal swine spermatocytes.…”

Section: Discussionmentioning

confidence: 99%

A Cytogenetic Study of Breeding Boars in Canada

Quach

Villagómez

Coppola

et al. 2009

Cytogenet Genome Res

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Chromosome abnormalities are well known for their negative impact on the reproductive performance of carriers. Such abnormalities could have severe effect on animal industries which rely heavily on efficient reproduction. We conducted a cytogenetic survey of breeder pigs from 4 different Canadian farms to investigate the frequency of chromosome abnormalities and to assess their reproductive impact on pig populations. Our study revealed that 50% of the ‘hypoprolific’ boars and 2.5% of the young boars raised for service in artificial insemination were carriers of chromosome anomalies while no chromosome defect was noted in any of the ‘proven’ breeder boars. G-banding technique to determine the type of abnormalities detected 3 previously unreported translocations involving chromosomes 1 and 6, chromosomes 10 and 13 and chromosomes 9 and 14. The reciprocal nature of these translocations was confirmed either using fluorescent in situ hybridization (FISH) technique or immunostaining for synaptonemal complex delineation and were named rcp(1;6)(p22,q12), rcp(10;13), and rcp(9;14) (p24;q27), respectively. Prolificacy of 1/6 and 10/13 translocation carriers was noted to be reduced by more than 40% compared to their normal counterparts while it was reduced by 26% in carriers of the 9/14 translocation. Carriers of 1/6 and 9/14 translocations displayed a higher repeat breeding tendency, compared to their herd average (5 and 16%, respectively). While for the 9/14 translocation the prevalence of stillbirths was lower than that in their herd [8.7 vs. 10.4% (p < 0.001)]. The present results, albeit based on a relatively small number of pigs, indicate that the prevalence of chromosome abnormalities could be much higher in Canadian pigs compared to that reported in European pigs and underline the urgent need to initiate cytogenetic screening programs as one of the effective ways to reduce reproductive problems in Canadian pig populations.

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Section: Discussionmentioning

confidence: 99%

A Cytogenetic Study of Breeding Boars in Canada

Quach

Villagómez

Coppola

et al. 2009

Cytogenet Genome Res

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“…To confirm this assumption, studies on several other translocations with such a very small rearranged chromosome should be carried out. Moreover, complementary pachytene pairing studies using immunofluorescence techniques Pinton et al, 2008] should also be carried out to assess the link between pairing abnormalities (presence of unsynapsed regions) and the increase of the non-disjunction rate.…”

Section: Characteristics Of the Rearrangementmentioning

confidence: 99%

Analysis Using Sperm-FISH of a Putative Interchromosomal Effect in Boars Carrying Reciprocal Translocations

Bonnet-Garnier

Guardia²,

Pinton³

et al. 2009

Cytogenet Genome Res

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The occurrence of interchromosomal effects (ICE) in reciprocal translocation carriers still remains contradictory in the human literature. We used the pig as an animal model to investigate whether the structure of the reciprocal translocations as well as the size and/or type of the chromosomes not involved in the rearrangement may influence the occurrence and the extent of ICE. Analyses of chromosomal sperm content by fluorescence in situ hybridization (FISH) using whole-chromosome painting probes for 7 chromosomes (1, 10, 11, 13, 18, X and Y) were carried out on sperm samples of 2 boars with normal semen parameters carrying different balanced reciprocal translocations: 38, XY, t(3;15)(q27;q13) or 38, XY, t(12;14)(q13;q21). One fertile boar with normal karyotype was also studied as a control. Aneuploidy rates for the 7 chromosomes were estimated by scoring 10,000 to 20,000 spermatozoa for each probe combination. No significant ICE was found except for chromosome 1 in the case of the t(3;15) translocation. Even if statistically significant, this ICE remained very weak and should have very little impact on the reproductive performance of the carrier boar. The size and/or type of chromosomes not involved in the translocation do not seem to have a major influence on the occurrence of ICE. The structure of the translocation could play a role, but complementary studies should be carried out to confirm this assumption.

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“…Only a few successful studies using the latter technique have been reported in farm animals such as cattle [2,5,8,[16][17], pigs [1,[14][15] and horses [3].…”

Section: Introductionmentioning

confidence: 99%

Modification of equine sperm chromatin decondensation method to use fluorescence in situ hybridization (FISH).

Bugno‐Poniewierska

Jabłońska²,

Słota³

2010

Folia Histochem Cytobiol.

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Abstract:Fluorescence in situ hybridization (FISH) is widely used in the study of chromosome structure and organization. Cytogenetic evaluation of chromosomes using FISH technique plays an increasingly important role in diagnosing karyotype changes in both somatic and reproductive cells. The aim of the study was to optimize the conditions of stallion sperm decondensation, which have a significant effect on the results of fluorescence in situ hybridization. Appropriate type and time of decondensation was chosen for the sperm of every stallion. It was found that decondensation performed using a preparation incubated in DTT solution for 1.5 minutes and in SDS solution for 10 seconds proved effective for stallions no. 1 and 2. An alternative decondensation method performed in an Eppendorf tube, with incubation in DTT solution for 1 minute and in SDS solution for 5 seconds proved effective for stallions no. 3 and 4. Decondensation using DTT and papain solution, a method successfully used for bull spermatozoa, proved inadequate for horse spermatozoa.

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Meiotic studies in an azoospermic boar carrying a Y;14 translocation

Cited by 27 publications

References 52 publications

A Cytogenetic Study of Breeding Boars in Canada

A Cytogenetic Study of Breeding Boars in Canada

Analysis Using Sperm-FISH of a Putative Interchromosomal Effect in Boars Carrying Reciprocal Translocations

Modification of equine sperm chromatin decondensation method to use fluorescence in situ hybridization (FISH).

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