Meiotic Proteins Bqt1 and Bqt2 Tether Telomeres to Form the Bouquet Arrangement of Chromosomes

Chikashige, Yuji; Tsutsumi, Chihiro; Yamane, Miho; Okamasa, Kasumi; Haraguchi, Tokuko; Hiraoka, Yasushi

doi:10.1016/j.cell.2006.01.048

Cited by 313 publications

(430 citation statements)

References 47 publications

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“…Consistent with the model, SUN proteins have been shown to interact with lamins and to serve as linkers to telomeres during meiosis. 22,24,25 The large N-terminal domains of KASH proteins are predicted to extend into the cytoplasm. In support, KASH proteins have been shown to interact with actin filaments, intermediate filaments and centrosomes (see below for examples).…”

Section: Kash and Sun Proteins Bridge The Nuclear Envelopementioning

confidence: 99%

“…Sad1p interacts with telomeres at the nuclear envelope and transports them to the spindle pole body. 24 Telomere localization requires dynein and microtubules in the cytoplasm. The forces of the microtubule motors are thought to be transmitted into the nucleus through Kms1p, a KASH protein at the outer nuclear membrane and Sad1p.…”

Section: The Role Of Microtubules Centrosomes and Dynein In Nuclear mentioning

confidence: 99%

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Communication between the cytoskeleton and the nuclear envelope to position the nucleus

Starr

2007

Mol. BioSyst.

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In most eukaryotic cells, the nucleus is localized to a specific location. This highlight article focuses on recent advances describing the mechanisms of nuclear migration and anchorage. Central to nuclear positioning mechanisms is the communication between the nuclear envelope and the cytoskeleton. All three components of the cytoskeleton-microtubules, actin filaments and intermediate filaments -are involved in nuclear positioning to varying degrees in different cell types. KASH proteins on the outer nuclear membrane connect to SUN proteins on the inner nuclear membrane. Together they transfer forces between the cytoskeleton and the nuclear lamina. Once at the outer nuclear membrane, KASH proteins can interact with the cytoskeleton. Nuclear migrations are a component of many cellular migration events and defects in nuclear positioning lead to human diseases, most notably lissencephaly.

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Section: Kash and Sun Proteins Bridge The Nuclear Envelopementioning

confidence: 99%

Section: The Role Of Microtubules Centrosomes and Dynein In Nuclear mentioning

confidence: 99%

Communication between the cytoskeleton and the nuclear envelope to position the nucleus

Starr

2007

Mol. BioSyst.

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“…A strain carrying Cnp1-YFP is a gift of Dr. M. Yoshida (RIKEN, Saitama, Japan), in which Cnp1-YFP is integrated at the leu1 ϩ locus, and is expressed under the nmt1 promoter (Matsuyama et al, 2006). A strain carrying Sid4 fused with monomeric RFP (mRFP) was crossed with a GFP-HA tagged strain for double staining of the SPB and the kinetochore (Chikashige et al, 2006). For tagging the Nuf2 carboxyl terminal with mRFP, the promoter region and ORF region of the nuf2 ϩ gene were cloned by PCR using genomic DNA as the template and fused with the mRFP coding gene in a vector containing the S. pombe lys1 ϩ gene.…”

Section: Strains and Plasmidsmentioning

confidence: 99%

Reconstruction of the Kinetochore during Meiosis in Fission Yeast Schizosaccharomyces pombe

Hayashi

Asakawa

Haraguchi

et al. 2006

MBoC

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During the transition from mitosis to meiosis, the kinetochore undergoes significant reorganization, switching from a bipolar to a monopolar orientation. To examine the centromere proteins that are involved in fundamental reorganization in meiosis, we observed the localization of 22 mitotic and 2 meiotic protein components of the kinetochore during meiosis in living cells of the fission yeast. We found that the 22 mitotic proteins can be classified into three groups: the Mis6-like group, the NMS (Ndc80-Mis12-Spc7) group, and the DASH group, based on their meiotic behavior. Mis6-like group proteins remain at the centromere throughout meiosis. NMS group proteins disappear from the centromere at the onset of meiosis and reappear at the centromere in two steps in late prophase. DASH group proteins appear shortly before metaphase of meiosis I. These observations suggest that Mis6-like group proteins constitute the structural basis of the centromere and that the NMS and DASH group proteins reassemble to establish the functional metaphase kinetochore. On the other hand, the meiosis-specific protein Moa1, which plays an important role in forming the meiotic monopolar kinetochore, is loaded onto the centromere significantly earlier than the NMS group, whereas another meiosis-specific protein, Sgo1, is loaded at times similar to the NMS group.

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“…The molecular correlates of non-disjunction in oocytes involve several mechanisms: chromosome condensation (29,30), pairing of homologous chromosomes (31,32), synaptonemal complex formation (33,34), recombination events (35,36), spindle formation (37,38) and meiotic checkpoints (39,40). Proteins actively functioning in these processes are essential for proper development, but their specific roles in oocyte maturation are difficult to unravel.…”

Section: Discussionmentioning

confidence: 99%

Origen parental, estado de no disyunción y recombinación meiótica del cromosoma 21 extra en el síndrome de Down: estudio en una muestra de población colombiana

et al. 2007

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Introduction. Free trisomy 21 is responsible for 95% of Down syndrome cases. Advanced maternal age and susceptible recombination patterns are recognized risk factors associated to Down syndrome. Maternal origin of trisomy occurs in approximately 90% of cases; paternal and mitotic origin share the remaining 10%. However, the recombination events that serve as a risk factors for trisomy 21 have not been carefully characterized. Objective. To analyze and validate observations in a sample of Colombian trysonomy 21 cases. Materials and methods. Twenty-two Colombian families were selected, each with one affected Down syndrome (free trisomy 21) child. Microsatellite polymorphisms were used as DNA markers to determine the parental/stage origin of non-disjunction and recombination events. Nonparametric tests were used to compare our results with those reported. Multiple correspondence analysis was used to outline different groups and their associations. Results. Distribution of trisomy 21 was 90.9% maternal, 4.5% paternal and 4.5% from mitotic origin, similar to distributions reported previously. However, we found differences in the frequency of maternal meiotic stage errors between the present study (46.1% meiosis I and 53.9% meiosis II) compared to those reported previously (70% meiosis I and 30% meiosis II). Multiple correspondence analyses showed association of either local recombination events or absence of recombination with specific non-disjunction stages. Conclusions. Recombination patterns found in this study support the hypothesis that susceptible chiasmate configurations are associated to maternal meiosis I and meiosis II errors. Nondisjunction frequencies between maternal meiotic stages need to be clarified in our population.Key words: Down syndrome; nondisjunction, genetic; trisomy; meiosis; recombination, genetic; microsatellite repeats.Origen parental, estado de no disyunción y recombinación meiótica del cromosoma 21 extra en el síndrome de Down: estudio en una muestra de población colombiana Introducción. La trisomía 21 libre es responsable del 95% de los casos de síndrome de Down. La edad materna y la recombinación son los principales factores de riesgo asociados con la concepción de estos individuos. El origen materno de la trisomía ocurre en el 90% de los casos, mientras que los casos de origen paterno y mitótico comparten un 10%. Por otra parte, la recombinación como factor de riesgo para la trisomía 21 no ha sido comprobada completamente. Objetivo. Analizar y validar estas observaciones en una muestra colombiana de casos con trisomía 21 libre. Materiales y métodos. Se estudiaron 22 afectados con síndrome de Down (trisomía libre) y sus respectivos padres. Se usaron marcadores microsatélites de ADN para determinar el origen en los progenitores, el estado de no disyunción y los eventos de recombinación. Por COMUNICACIÓN BREVE 142 Biomédica 2007;27:141-8 Ramírez NJ, Belalcazar HM, Yunis JJ et al medio de pruebas no paramétricas se compararon los resultados con los reportados en la literatura. Se re...

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Meiotic Proteins Bqt1 and Bqt2 Tether Telomeres to Form the Bouquet Arrangement of Chromosomes

Cited by 313 publications

References 47 publications

Communication between the cytoskeleton and the nuclear envelope to position the nucleus

Communication between the cytoskeleton and the nuclear envelope to position the nucleus

Reconstruction of the Kinetochore during Meiosis in Fission Yeast Schizosaccharomyces pombe

Origen parental, estado de no disyunción y recombinación meiótica del cromosoma 21 extra en el síndrome de Down: estudio en una muestra de población colombiana

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