Meiotic non-disjunction induced by fission neutrons relative to X-rays observed in mouse secondary spermatocytes I. The response of different cell stages to a single radiation dose

Russo, Antonella; Pacchierotti, Francesca; Metalli, P.

doi:10.1016/0027-5107(83)90132-x

Cited by 13 publications

(6 citation statements)

References 34 publications

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“…A direct cytogenetic method of measuring the induction of aneuploidy in vivo in germ cells of male mice [Evans et al, 1964;Beatty et al, 1975;Szemere and Chandley, 19751 has been recently standardized in our laboratory [Russo et al, 1981;Pacchierotti et al, 1982Pacchierotti et al, , 1983aRusso et al, 1983;Pacchierotti et al, in preparation]. Metaphase I1 preparations are scored to determine the frequency of hyper-haploid cells.…”

Section: Introductionmentioning

confidence: 99%

Nondisjunction induced in mouse spermatogenesis by chloral hydrate, a metabolite of trichloroethylene

1984

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The effects of chloral hydrate (CH), an in vivo metabolite of trichloroethylene, have been evaluated by cytogenetic observations of mouse secondary spermatocytes after ip treatment with 82.7, 165.4, or 413.5 mg/kg bw. Hyper-haploid metaphases have been scored to determine whether previous observations in various nonmammalian organisms about an effect of this drug on the mitotic spindle could be confirmed in mice. At each dose, the frequencies of hyper-haploid cells have been estimated to assess the response of pachytene, preleptotene, premeiotic, and staminal gonial cells. Significant increases above the control value have been observed particularly after treatment of actively dividing gonial cells, confirming the results obtained with the same batch of the drug in a parallel collaborative investigation with Aspergillus nidulans. Thus: a) chloral hydrate has been shown to be effective in inducing nondisjunction in a mammalian system; b) a prevalent action on the mitotic spindle has been confirmed and quantified; and c) the usefulness of parallel investigations with different methods is stressed, particularly to collect information about the mechanisms of induction of nondisjunction events.

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Section: Introductionmentioning

confidence: 99%

Nondisjunction induced in mouse spermatogenesis by chloral hydrate, a metabolite of trichloroethylene

1984

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“…For details on the criteria of scoring and evaluation of recorded metaphases, see Russo et al [1983].…”

Section: Detection Of Aneuploidy In the Male Mousementioning

confidence: 99%

“…In the cytogenetic analysis of the preparations, only euploid (n=20) and hyperhaploid (n = 21, n = 22) metaphases were considered to be informative, and an index of hyperhaploidy was evaluated by considering the ratio between hyperhaploid metaphases and the sum of euploid plus hyperhaploid cells on a total of 100 metaphases scored per animal. For details on the criteria of scoring and evaluation of recorded metaphases, see Russo et al [1983].…”

Section: Detection Of Aneuploidy In the Male Mousementioning

confidence: 99%

“…In the mouse, a cytogenetic method based on chromosomal counting in secondary spermatocytes of animals treated with the compound under study was used. This method was standardized and validated in our laboratories [Russo et al, 1983;Pacchierotti et al, 1983;Russo et al, 1984;Pacchierotti, 19881, and it is proposed to be a very efficient method for the detection of germinal aneuploidy in mammals [Allen et al, 19861.…”

Section: Introductionmentioning

confidence: 99%

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Nitrilotriacetic acid (NTA) induces aneuploidy in drosophila and mouse germ‐line cells

et al. 1988

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The ability of nitrilotriacetic acid (NTA) to induce aneuploidy was studied in the germ line of both Drosophilu and the mouse. The Free Inverted X Chromosomes (FIX) genetic system, adopting a brooding scheme, was used to detect induced aneuploidy in Drosophil~, and a cytogenetic method based on chromosomal counting in secondary spermatocytes was used in the mouse.In Drosophilu a highly significant (P < 0.001) increase of aneuploidies was produced by NTA (5 X M), which was greater than that produced by colchicine (7.5 X M) and 5-fluorodeoxyuridine M), which were used as positive controls. Brooding effects were observed with NTA, which produced a maximum induction of chromosomal gain in brood I, suggesting a possible stage-specific action during meiosis. The ability of NTA (275 mglkg body weight) to induce meiotic aneuploidy (hyperhaploidy) also was confirmed in the mouse (P < 0.001), where all the aneuploidies detected were attributable to treatment of the metaphase I stage.

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“…Radiation-induced chromosome nondisjunction has been described in female mice by Tease (1982a;1982b) and in male mice by Russo, et al (1983 damage to chromosome structure may be responsible for non-disjunction as well as chromosome breakage. Cyclophosphamide, an antineoplastic drug, also increased the frequency of both hyperhaploid cells and those with chromosome breaks in mouse secondary spermatocytes (Pacchierotti,et al 19 83).…”

Section: S~and2ngs~~--n2n=gi~iynandti2nmentioning

confidence: 99%

Origin of human trisomy 21 mosaicism

Waggoner¹

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Meiotic non-disjunction induced by fission neutrons relative to X-rays observed in mouse secondary spermatocytes I. The response of different cell stages to a single radiation dose

Cited by 13 publications

References 34 publications

Nondisjunction induced in mouse spermatogenesis by chloral hydrate, a metabolite of trichloroethylene

Nondisjunction induced in mouse spermatogenesis by chloral hydrate, a metabolite of trichloroethylene

Nitrilotriacetic acid (NTA) induces aneuploidy in drosophila and mouse germ‐line cells

Origin of human trisomy 21 mosaicism

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