Meiotic maturation, fertilization and effect of ultraviolet irradiation on the fertilizing sperm in the Japanese scallop

Li, Q. I.; Osada, Makoto; Kashihara, Masaru; Hirohashi, Ken; Kijima, Akihiro

doi:10.1046/j.1444-2906.2000.00063.x

Cited by 5 publications

(4 citation statements)

References 10 publications

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“…Compared with normal eggs, the developmental progress of gynogenetic eggs clearly lagged. Similar results have also been observed also in gynogenetic zygotes of the Pacific oyster, 31 Japanese scallop, 35 amago salmon, 16 and rainbow trout, 17 indicating that the optimum treatment time for chromosome diploidization employed in triploid or tetraploid production is not always applicable in the induction of gynogenetic diploid. 17…”

Section: Discussionmentioning

confidence: 68%

“…The morphological features of the early fertilization events observed in the normal abalone eggs are fundamentally similar to that described for the bivalves. 25–35 After the condensation of the female and male pronuclei, two groups of chromosomes derived from the female and male pronuclei became closely associated and situated on the metaphase plate of the first cleavage. It appears that no zygotic nucleus was formed in the abalone as reported for the bivalves of M. edulis , 25,26 Spisula solidissima , 27,28 Mulinia lateralis 33 and Placopecten magellanicus .…”

Section: Discussionmentioning

confidence: 99%

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Cytological studies on artificially induced gynogenesis in the Pacific abalone

Osada

Kashihara³

et al. 2000

Fisheries Sci

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SUMMARY: To obtain useful information about the mechanism of the induction of gynogenesis in the Pacific abalone, nuclear behavior in normal and gynogenetic eggs was examined during meiosis and first cleavage. After its incorporation into the egg cytoplasm, the UV‐irradiated sperm nucleus gradually dispersed and eventually developed into the male pronucleus, behaving like a normal sperm until contact with the female pronucleus. At mitotic prophase, the male pronucleus in the gynogenetic eggs did not form chromosomes like the female pronucleus, but became a dense chromatin body, which did not participate in karyokinesis. At completion of cytokinesis of the first cleavage, the UV‐irradiated sperm nucleus was seen in the cytoplasm of one of the two blastomeres near the cleavage furrow. As a clear haploid mode was observed in the frequency distribution of chromosome numbers in gynogenetic larval cells, it is suggested that the paternal genome in the gynogenetic eggs does not contribute to the next generation.

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Section: Discussionmentioning

confidence: 68%

Section: Discussionmentioning

confidence: 99%

Cytological studies on artificially induced gynogenesis in the Pacific abalone

Osada

Kashihara³

et al. 2000

Fisheries Sci

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“…For cytological examination, eggs were collected from each group at 5 min intervals up to 2 h after the addition of sperm, and fixed in 2% formalin in seawater at 4 • C. After rinsing three times with 0.1 M phosphate buffer containing 8% sucrose, the samples were stained with DAPI (4 , 6-diamidino-2-phenylindole dihydrochloride) following Li et al (2000d). Cytological events in more than 100 DAPI-stained eggs per sample were observed under an Olympus fluorescence microscope BH-2.…”

Section: Cytological Observation Of Nuclear Behaviormentioning

confidence: 99%

Induction of gynogenetic diploids and cytological studies in the zhikong scallop,Chlamys farreri

Pan

et al. 2004

Aquat. Living Resour.

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The induction of gynogenetic diploids during meiosis II in the scallop Chlamys farreri was attempted using the treatments of cytochalasin B (0.5 µg ml −1 ; CB) and 6-dimethylaminopurine (60 µg ml −1 ; 6-DMAP). The effects of CB and 6-DMAP treatments on meiosis and fertilization of eggs were also examined. Haploid gynogenesis was induced by sperm which were ultraviolet-irradiated for 30 s at an intensity of 2561 µW cm −2 s −1 . CB and 6-DMAP treatments were highly effective in suppressing meiosis II, yielding 19% and 28% gynogenetic diploids, respectively. Compared with the CB treatment, the 6-DMAP treatment produced more expected D-shaped larvae. Cytological observations revealed that 6-DMAP inhibited pronuclear movements and the segregation of chromosomes, resulting in the formation of one big diploid female pronucleus with strong fluorescence, while CB inhibited cytokinesis, resulting in two female pronuclei formations. The UV-irradiated sperm nucleus developed into a male pronucleus, but did not participate in karyokinesis at mitotic anaphase, suggesting that gynogenetic diploids were induced in the scallop.

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“…O objetivo dos métodos de indução à triploidia em moluscos é promover a interrupção da meiose, quer seja pela retenção do primeiro corpo polar (Meiose I) ou do segundo corpo polar (Li et al 2000). Outra forma de se obter triploides é através do cruzamento de tetraploides e diploides (Guo et al 2009;Piferrer et al 2009).…”

Section: Introductionunclassified

SOBREVIV�NCIA AP�S INDU��O � TRIPLOIDIA EM Anomalocardia brasiliana (VENERIDAE) POR TRATAMENTO QU�MICO, HIPOT�NICO E T�RMICO

Lavander

2018

Rev. Bras. Eng. Pesca

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A poliploidia � uma alternativa para melhorar o desempenho zoot�cnico e � amplamente empregada na aquicultura mundial. No Brasil, o emprego de t�cnicas na escala comercial limita-se a uma esp�cie ex�tica de ostra Crassostrea gigas. O objetivo deste estudo foi avaliar a sobreviv�ncia ap�s aplica��o de protocolos de indu��o � triploidia em Anomalocardia brasiliana atrav�s de agentes qu�micos, como Citocalasina B (CB) e 6 - Dimetilaminopurina (6-DMAP), e m�todos f�sicos, por choque t�rmico frio e choque hiposm�tico. A indu��o � triploidia por CB foi realizada a 1 mg. L-1, enquanto que a 6-Dimetilaminopurina a 450 �mol.L-1. As indu��es por choque t�rmico frio foram realizadas a 2, 5 e 7�C, e as indu��es por choque hiposm�tico a 12� de salinidade. As indu��es qu�micas � triploidia apresentaram baixa sobreviv�ncia larval ap�s as primeiras 24 horas, diferentemente dos tratamentos f�sicos, estes obtiveram mais de 90% de sobreviv�ncia, assim como o tratamento controle. Estes resultados demonstram que os tratamentos f�sicos neste per�odo s�o menos agressivos aos ovos e larvas. Por�m novos estudos dever�o ser realizados, a fim de aprimorar os protocolos para obten��o de poliploides para esp�cie.

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Meiotic maturation, fertilization and effect of ultraviolet irradiation on the fertilizing sperm in the Japanese scallop

Cited by 5 publications

References 10 publications

Cytological studies on artificially induced gynogenesis in the Pacific abalone

Cytological studies on artificially induced gynogenesis in the Pacific abalone

Induction of gynogenetic diploids and cytological studies in the zhikong scallop,Chlamys farreri

SOBREVIV�NCIA AP�S INDU��O � TRIPLOIDIA EM Anomalocardia brasiliana (VENERIDAE) POR TRATAMENTO QU�MICO, HIPOT�NICO E T�RMICO

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