Meiotic gene conversion and crossing over: Their relationship to each other and to chromosome synapsis and segregation

Engebrecht, JoAnne; Hirsch, Jeanne P.; Roeder, G. Shirleen

doi:10.1016/0092-8674(90)90267-i

Cited by 163 publications

(77 citation statements)

References 32 publications

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“…mre2D (TNY047) and mre2N (TNY048) cells harbouring multi-copy plasmids, YEpMRE2 (MRE2) and pSMT1 (MER2) or YEp24 (vector), respectively, were examined for prototroph formation after induction of meiosis as described in Table 1 §. that the insert does not contain the MRE2 gene and that the suppression activity was encoded in a 2.0 kb region containing the previously identified MER2 gene (Engebrecht et al 1990;Cool & Malone 1992).…”

Section: Figurementioning

confidence: 99%

“…It has been shown that MER1 is required for the meiosis-specific splicing of MER2 pre-mRNA (Engebrecht et al 1991;Nandabalan et al 1993;Nandabalan & Roeder 1995). The MER2 gene is required for chromosome synapsis and initiation of meiotic recombination (Engebrecht et al 1990;Cool & Malone 1992;Rockmill et al 1995).…”

Section: Introductionmentioning

confidence: 99%

“…Two types of recombination occur between homologues, crossovers with exchanged flanking chromosome arms and noncrossovers without such an exchange. In combination with sister chromatid cohesion, crossover events are essential for the proper segregation of homologues (Baker et al 1976;Bell & Byers 1982;Carpenter 1988;Engebrecht et al 1990;Storlazzi et al 1995).…”

Section: Introductionmentioning

confidence: 99%

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**Involvement of the MRE2 gene of yeast in formation of meiosis‐specific double‐strand breaks and crossover recombination through RNA splicing**

Nakagawa

Ogawa

1997

Genes to Cells

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Section: Figurementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

**Involvement of the MRE2 gene of yeast in formation of meiosis‐specific double‐strand breaks and crossover recombination through RNA splicing**

Nakagawa

Ogawa

1997

Genes to Cells

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“…Similarly, in the Saccharomyces cerevisiae zipl meiotic mutant, the absence of synapsis is associated with a complete elimination of crossover interference (Sym and Roeder, 1994). The SC may also be required for the conversion of crossover events into chiasmata, the cytological manifestation of crossing over, which ensure proper disjunction of homologues at meiosis I (Maguire, 1978;Engebrecht et al, 1990;Rockmill and Roeder, 1990).…”

Section: Introductionmentioning

confidence: 99%

Protein-protein interactions in the synaptonemal complex.

Tarsounas

Pearlman

Gasser

et al. 1997

MBoC

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In mammalian systems, an approximately M(r) 30,000 Cor1 protein has been identified as a major component of the meiotic prophase chromosome cores, and a M(r) 125,000 Syn1 protein is present between homologue cores where they are synapsed and form the synaptonemal complex (SC). Immunolocalization of these proteins during meiosis suggests possible homo- and heterotypic interactions between the two as well as possible interactions with yet unrecognized proteins. We used the two-hybrid system in the yeast Saccharomyces cerevisiae to detect possible protein-protein associations. Segments of hamsters Cor1 and Syn1 proteins were tested in various combinations for homo- and heterotypic interactions. In the cause of Cor1, homotypic interactions involve regions capable of coiled-coil formation, observation confirmed by in vitro affinity coprecipitation experiments. The two-hybrid assay detects no interaction of Cor1 protein with central and C-terminal fragments of Syn1 protein and no homotypic interactions involving these fragments of Syn1. Hamster Cor1 and Syn1 proteins both associate with the human ubiquitin-conjugation enzyme Hsubc9 as well as with the hamster Ubc9 homologue. The interactions between SC proteins and the Ubc9 protein may be significant for SC disassembly, which coincides with the repulsion of homologs by late prophase I, and also for the termination of sister centromere cohesiveness at anaphase II.

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“…Several studies employing meiotic mutants have shown that gene conversion and reciprocal recombination can be separated (e.g. Engebrecht et al 1990;Xu et al 1995). Other explanations for the disparity between R1 and R2 include a loss of R1 structure (perhaps due to intractable recombinant intermediates), or the poor accessibility of site X(b) to restriction enzyme in DNA extracted during RTG, but not during meiosis.…”

mentioning

confidence: 99%

Switching yeast from meiosis to mitosis: double‐strand break repair, recombination and synaptonemal complex

et al. 1997

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Background: When Saccharomyces cerevisiae cells that have begun meiosis are transferred to mitotic growth conditions ('return-to-growth', RTG), they can complete recombination at high meiotic frequencies, but undergo mitotic cell division and remain diploid. It was not known how meiotic recombination intermediates are repaired following RTG. Using molecular and cytological methods, we investigated whether the usual meiotic apparatus could repair meiotically induced DSBs during RTG, or whether other mechanisms are invoked when the developmental context changes.

show abstract

Meiotic gene conversion and crossing over: Their relationship to each other and to chromosome synapsis and segregation

Cited by 163 publications

References 32 publications

**Involvement of the MRE2 gene of yeast in formation of meiosis‐specific double‐strand breaks and crossover recombination through RNA splicing**

**Involvement of the MRE2 gene of yeast in formation of meiosis‐specific double‐strand breaks and crossover recombination through RNA splicing**

Protein-protein interactions in the synaptonemal complex.

Switching yeast from meiosis to mitosis: double‐strand break repair, recombination and synaptonemal complex

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