Meiotic consequences of selfing in grass pea (Lathyrus sativus L.) autotetraploids in the advanced generations: Cytogenetics of chromosomal rearrangement and detection of aneuploids

Talukdar, Dibyendu

doi:10.1007/s13237-012-0059-z

Cited by 10 publications

(5 citation statements)

References 27 publications

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“…It has been shown that descending aneuploidy occurred mostly in early diverging papilionoid lineages (to which lupins belong), especially in species with low basic chromosome numbers [33]. Aneuploidy is exemplified in the Arachis genus (2n = 2x = 18) [34], Lathyrus [35], and Medicago sections [36]. Both dysploidy and aneuploidy in lupins cannot be ruled out, considering that polyploidy is associated with chromosome number reduction in core papilionoids [33,37].…”

Section: Discussionmentioning

confidence: 99%

Impact of Chromosomal Rearrangements on the Interpretation of Lupin Karyotype Evolution

Susek

Bielski

Czyż

et al. 2019

Genes

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Plant genome evolution can be very complex and challenging to describe, even within a genus. Mechanisms that underlie genome variation are complex and can include whole-genome duplications, gene duplication and/or loss, and, importantly, multiple chromosomal rearrangements. Lupins (Lupinus) diverged from other legumes approximately 60 mya. In contrast to New World lupins, Old World lupins show high variability not only for chromosome numbers (2n = 32–52), but also for the basic chromosome number (x = 5–9, 13) and genome size. The evolutionary basis that underlies the karyotype evolution in lupins remains unknown, as it has so far been impossible to identify individual chromosomes. To shed light on chromosome changes and evolution, we used comparative chromosome mapping among 11 Old World lupins, with Lupinus angustifolius as the reference species. We applied set of L. angustifolius-derived bacterial artificial chromosome clones for fluorescence in situ hybridization. We demonstrate that chromosome variations in the species analyzed might have arisen from multiple changes in chromosome structure and number. We hypothesize about lupin karyotype evolution through polyploidy and subsequent aneuploidy. Additionally, we have established a cytogenomic map of L. angustifolius along with chromosome markers that can be used for related species to further improve comparative studies of crops and wild lupins.

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Section: Discussionmentioning

confidence: 99%

Impact of Chromosomal Rearrangements on the Interpretation of Lupin Karyotype Evolution

Susek

Bielski

Czyż

et al. 2019

Genes

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“…Although polyploidy has been studied in Lathyrus spp. (Khawaja et al 1998;Talukdar 2010Talukdar , 2012, mitotic fidelity in aneuploid genomes was not known in grass pea. Characterization of aneuploid chromosomes through molecular karyotyping for the first time in grass pea thus assumes significance.…”

Section: Discussionmentioning

confidence: 99%

RETRACTED ARTICLE: Karyotype analysis and identification of extra chromosomes in primary aneuploid stocks of grass pea (Lathyrus sativus L.) by fluorescence chromosome banding

2016

Caryologia

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Prominent primary aneuploid stocks namely seven primary trisomics, seven primary tetrasomics, and six double trisomic types were earlier developed in grass pea (Lathyrus sativus L.), a hardy legume. Despite distinct morphological features, identity and nature of their extra chromosome(s) were elusive, hampering assignment of desirable breeding traits into specific linkage groups. The present study aims to analyze the banding pattern and to reveal the identity of extra chromosome(s) involved in these 20 aneuploid types in grass pea. Conventional orcein banding was first done using the root-tip squash technique in all aneuploids along with disomic (2n = 2x = 14) parent, and karyomorphological features were noted. Chromosomes were classified following the total length of individual chromosomes and arranged in order of decreasing sizes, keeping their centromeres in a straight line. DNA-base specific chromomycin A3 (CMA) and 4,6ʹdiamidino-2-phenylindole (DAPI) banding pattern were finally employed to convincingly distinguish the chromosomes from each other. Molecular banding and idiograms revealed unique CMA and DAPI banding pattern in each of the seven chromosome pairs and identified extra chromosome(s) involved in aneuploidy. Fluorescence banding also showed differential CMA-specific GC-rich repeat region and DAPI-specific AT-rich repeat regions in chromosomes with the longest chromosome bearing GC-rich nucleolar organizing region. No polymorphism within homologous pairs was found. The result confirmed the primary nature of present aneuploids and identified the additional chromosome(s) in aneuploids. Based on the extra chromosome(s) involved, new designation of grass pea aneuploids has now been proposed. The results have immense significance in location of desired traits or mutations on specific linkage groups of grass pea.

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“…Dry and uniformly sized seeds of grass pea variety BioR-231 (diploid, 2n = 2x = 14), an induced autotetraploid line (2n = 4x = 28), and a triploid population (2n = 3x = 21) derived from diploid × tetraploid crosses (Talukdar, 2010b(Talukdar, , 2012c were sterilized in 2% sodium hypochlorite for 1 h, thoroughly rinsed in sterile distilled water, soaked in distilled water for 12 h, and germinated on moist filter papers at 25 °C in the dark. Germinated seedlings were randomly placed in polythene pots (12 cm in height and 10 cm in diameter, 10 plants pot -1 ) containing 200 mL of Hoagland's No.…”

Section: Plant Materials and Treatment Protocolsmentioning

confidence: 99%

“…Although Cd is considered as a potent mutagenic agent, nothing is known about its effect on plant genomes with higher ploidy. Both autotetraploid and triploid lines have been developed in grass pea (Talukdar, 2010b(Talukdar, , 2012c, providing an excellent opportunity to investigate plants' intrinsic responses to metal stress at different ploidy levels. The main objectives of present work were to: 1) ascertain roles of nuclear ploidy in modulating antioxidant defense response, and 2) assess chromotoxic/genotoxic damage in response to Cd treatment at 3 different ploidy levels: diploid, triploid, and tetraploid.…”

Section: Introductionmentioning

confidence: 99%

Increasing nuclear ploidy enhances the capability of antioxidant defense and reduces chromotoxicity in Lathyrus sativus roots under cadmium stress

Talukdar¹

2014

Turk J Bot

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The modulation of antioxidant defense and cytogenetic parameters was studied in Lathyrus sativus L. roots at 3 different ploidies under a control treatment (0 µM) and 5, 10, 25, and 50 µM of CdCl 2 in nutrient media. Root growth was inhibited in diploids from 10 µM and in triploid from 25 µM. Growth was normal in tetraploid roots. Although Cd accumulated in ascending order of ploidy, perturbation of antioxidant defense was the severest in diploid, moderate in triploids, and not observed in tetraploid roots even at 50 µM. Low antioxidant enzyme activities led to overaccumulation of H 2 O 2 , consequently resulting in oxidative damage through membrane lipid peroxidation in treated diploid roots. Cd-induced chromotoxicity was exhibited by low mitotic fidelity in diploid chromosomes and to some extent in triploids. DNA base-specific fluorescent chromosome banding revealed GC to AT transition and even perturbation of the GC-rich region in the nucleolar organizing chromosome in diploid, presumably facilitating chromosome breakage at a lower ploidy level due to Cd treatment. By contrast, the normal to enhanced GC region was associated with a low percentage of chromosome breakage and other anomalies at higher ploidies exposed to Cd. Results suggest better tolerance of grass pea genotypes to Cd at elevated ploidy levels.

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Meiotic consequences of selfing in grass pea (Lathyrus sativus L.) autotetraploids in the advanced generations: Cytogenetics of chromosomal rearrangement and detection of aneuploids

Cited by 10 publications

References 27 publications

Impact of Chromosomal Rearrangements on the Interpretation of Lupin Karyotype Evolution

Impact of Chromosomal Rearrangements on the Interpretation of Lupin Karyotype Evolution

RETRACTED ARTICLE: Karyotype analysis and identification of extra chromosomes in primary aneuploid stocks of grass pea (Lathyrus sativus L.) by fluorescence chromosome banding

Increasing nuclear ploidy enhances the capability of antioxidant defense and reduces chromotoxicity in Lathyrus sativus roots under cadmium stress

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