Meiotic cohesin complexes are essential for the formation of the axial element in mice

Llano, Elena; Herrán, Yurema; García‐Tuñón, Ignacio; Gutiérrez-Caballero, Cristina; Álava, Enrique de; Barbero, José Luís; Schimenti, John C.; Rooij, Dirk G. de; Sánchez-Martı́n, Manuel; Pendás, Alberto M.

doi:10.1083/jcb.201201100

Cited by 111 publications

(176 citation statements)

References 60 publications

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“…Although SC assembly occurs solely between sisters in Rec8 knockout spermatocytes as reported (Bannister et al 2004;Xu et al 2005), Rad21L knockout spermatocytes assemble SC at both intersisters and interchromosomes ( Fig. 4B; Llano et al 2012). In contrast to the results using mice with a single mutation, Rec8/Rad21L doubleknockout mice (male and female) did not form either cohesin axes or chromosome axes, including SYCP3 ( Fig.…”

Section: Cohesin and Chromosome Architecturecontrasting

confidence: 50%

“…3J,K), suggesting that RAD21L and REC8 redundantly function in forming the cohesin axis, a base for the assembly of the AE (Fig. 7, below ;Llano et al 2012). Immunogold electron microscopic analyses revealed that cohesin complexes localize on the innermost sides of the two lateral elements in wild-type bivalents (Fig.…”

Section: Cohesin and Chromosome Architecturementioning

confidence: 99%

“…Meiotic cohesin is crucial not only for sister chromatid cohesion but also to act as a structural basis for axial element (AE) formation and synaptonemal complex (SC) assembly during prophase I (Klein et al 1999;Zickler and Kleckner 1999;Prieto et al 2001;Page and Hawley 2004;Novak et al 2008;Llano et al 2012). Recent studies in mice have identified a novel meiosis-specific a-kleisin subunit of cohesin, RAD21L (Herran et al 2011;Ishiguro et al 2011;Lee and Hirano 2011), in addition to the previously identified REC8 (Bannister et al 2004;Xu et al 2005).…”

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Meiosis-specific cohesin mediates homolog recognition in mouse spermatocytes

Ishiguro¹,

et al. 2014

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During meiosis, homologous chromosome (homolog) pairing is promoted by several layers of regulation that include dynamic chromosome movement and meiotic recombination. However, the way in which homologs recognize each other remains a fundamental issue in chromosome biology. Here, we show that homolog recognition or association initiates upon entry into meiotic prophase before axis assembly and double-strand break (DSB) formation. This homolog association develops into tight pairing only during or after axis formation. Intriguingly, the ability to recognize homologs is retained in Sun1 knockout spermatocytes, in which telomere-directed chromosome movement is abolished, and this is the case even in Spo11 knockout spermatocytes, in which DSB-dependent DNA homology search is absent. Disruption of meiosis-specific cohesin RAD21L precludes the initial association of homologs as well as the subsequent pairing in spermatocytes. These findings suggest the intriguing possibility that homolog recognition is achieved primarily by searching for homology in the chromosome architecture as defined by meiosis-specific cohesin rather than in the DNA sequence itself.

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Section: Cohesin and Chromosome Architecturecontrasting

confidence: 50%

Section: Cohesin and Chromosome Architecturementioning

confidence: 99%

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confidence: 99%

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Meiosis-specific cohesin mediates homolog recognition in mouse spermatocytes

Ishiguro¹,

et al. 2014

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“…It is possible that, in this case, the cohesin core is sufficient to allow the binding of the TFs (Pelttari et al 2001). Disruption of the AEs and major parts of the cohesin core, as it is found in the kleisin double mutant Rec8 -/-Rad21l -/-, however, finally leads to a failure in SYCP1 association as well (Llano et al 2012). In the wild-type situation, SYCE3 is recruited to SYCP1 in the next step followed by SYCE1.…”

Section: Second Step: the Assembly Of The Central Regionmentioning

confidence: 99%

“…SMC1ß (Revenkova et al 2004) or REC8 (Bannister et al 2004), affects AE morphology and assembly. It even completely abolishes the AE formation if the two meiotic kleisin subunits REC8 and RAD21L are depleted in a double knock-out (Llano et al 2012). This suggests that the cohesion core is necessary for the assembly of a defined AE.…”

Section: First Step: the Assembly Of The Chromosomal Axes And The Aximentioning

confidence: 99%

Evolutionary history of the mammalian synaptonemal complex

et al. 2016

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STAG3‐mediated stabilization of REC8 cohesin complexes promotes chromosome synapsis during meiosis

et al. 2014

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Cohesion between sister chromatids in mitotic and meiotic cells is promoted by a ring-shaped protein structure, the cohesin complex. The cohesin core complex is composed of four subunits, including two structural maintenance of chromosome (SMC) proteins, one a-kleisin protein, and one SA protein. Meiotic cells express both mitotic and meiosis-specific cohesin core subunits, generating cohesin complexes with different subunit composition and possibly separate meiotic functions. Here, we have analyzed the in vivo function of STAG3, a vertebrate meiosis-specific SA protein. Mice with a hypomorphic allele of Stag3, which display a severely reduced level of STAG3, are viable but infertile. We show that meiocytes in homozygous mutant Stag3 mice display chromosome axis compaction, aberrant synapsis, impaired recombination and developmental arrest. We find that the three different a-kleisins present in meiotic cells show different dosage-dependent requirements for STAG3 and that STAG3-REC8 cohesin complexes have a critical role in supporting meiotic chromosome structure and functions.

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Meiotic cohesin complexes are essential for the formation of the axial element in mice

Abstract: Loss of both meiosis-specific kleisins in mice reveals conserved functions of the meiotic cohesin complexes in axial element formation during spermatogenesis.

Cited by 111 publications

References 60 publications

Meiosis-specific cohesin mediates homolog recognition in mouse spermatocytes

Meiosis-specific cohesin mediates homolog recognition in mouse spermatocytes

Evolutionary history of the mammalian synaptonemal complex

STAG3‐mediated stabilization of REC8 cohesin complexes promotes chromosome synapsis during meiosis

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