Meiotic and developmental competence of prepubertal and adult swine oocytes

Marchal, R.; Feugang, Jean M.; Perreau, C.; Venturi, Éric; Terqui, M.; Mermillod, Pascal

doi:10.1016/s0093-691x(01)00539-8

Cited by 139 publications

(115 citation statements)

References 43 publications

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“…In general, prepubertal oocytes show reduced developmental competence, when compared with results obtained with adult oocytes (Armstrong, 2001). Indeed, the defective capacity of these oocytes to develop up to blastocyst at a rate equivalent to that of adult oocytes has been reported by several other studies in cattle (Presicce et al, 1997), in sheep (Ledda et al, 1997), in pig (Marchal et al, 2001) and more recently in goats stimulated hormonally (Leoni et al, 2009).…”

Section: Discussionmentioning

confidence: 85%

In vitro developmental competence of prepubertal goat oocytes cultured with recombinant activin-A

Hammami

Izquierdo

Catalá

et al. 2014

Animal

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The present study was designed to evaluate the effect of activin-A during the in vitro oocyte maturation (IVM) and in vitro embryo culture (IVC) on nuclear maturation, blastocyst yield and blastocyst quality of prepubertal goat oocytes. In Experiment 1, three groups of oocytes were used during the IVM of prepubertal goat oocytes to determine the optimal concentration of recombinant human activin-A added to the maturation medium. Cumulus-oocyte complexes were matured in an IVM medium containing 0, 10 and 100 ng/ml (groups A0, A10 and A100), fertilized and in vitro cultured using standard procedures. In Experiment 2, the addition of 10 ng/ml activin-A at IVM (A10A0), IVC (A0A10) or IVM + IVC (A10A10) was studied and compared with the control group (A0A0). Results of the first experiment demonstrated that the addition of activin-A yielded similar percentages of maturation (⩽71.0%) and blastocyst formation rates (⩽24.9%) than the control group (A0). Experiment 2 showed that exposure of prepubertal goat oocytes to an IVC medium containing 10 ng/ml activin-A (A0A10) significantly increased the rates of development to the blastocyst stage, as compared with the control group (A0A0) (19.5 ± 2.21% v. 13.1 ± 2.37%, respectively; P < 0.05). With regard to the blastocyst quality, total number of cells, inner cell mass (ICM) and trophectoderm of prepubertal goat embryos produced in the presence of activin-A did not differ significantly among experimental groups. In summary, these results indicate that supplementation of the IVC medium with activin-A enhances embryo development of prepubertal goat oocytes.

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Section: Discussionmentioning

confidence: 85%

In vitro developmental competence of prepubertal goat oocytes cultured with recombinant activin-A

Hammami

Izquierdo

Catalá

et al. 2014

Animal

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“…It has been reported that oocytes of prepubertal gilts appeared less meiotically and developmentally competent than those of their more adult counterparts. A lower reproductive potential was observed also in primiparous sows, therefore, the use of pubertal but cycling gilts was recommended for IVF and breeding programs (Marchal et al, 2001;Brussow et al, 2002;Ratky et al, 2005). Higher meiotic and developmental competence observed in oocytes after the onset of puberty is acquired gradually during multiple oestrous cycles (Bagg et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

Oestrous cycle stage influences the morphology and maturation of porcine oocytes in vitro

Machatkova¹,

Hulinska²,

Horakova³

et al. 2008

Vet. Med. - Czech

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ABSTRACT:The objective of the study was to characterize the effect of the oestrous cycle stage on the yield, morphology and meiotic competence of porcine oocytes. A total of 46 cycling gilts, at 8.5-9 months of age, were used as oocyte donors. Their oestrous cycle was synchronized by Regumate and the onset of oestrus was checked (Day 0). The gilts were slaughtered at the early (Days 1-5), middle (6-10) and late (11-14) luteal or early and late (20-21) follicular phase. Oocytes were isolated separately from medium (5-9 mm) and small (≤ 4 mm) follicles. Cumulus-oocyte complexes with dark, evenly granulated cytoplasm and at least two compact layers of cumulus cells were selected as useful for maturation. They were matured by a standard protocol, denuded from cumulus cells, fixed in glutaraldehyde, stained with 33258-Hoechst and examined by epifluorescence. The oocytes collected from small and medium follicles differed in their yield, morphology and meiotic competence regardless of the phase. The mean number (± S.E.M.) of oocytes isolated per donor was higher (187.7 ± 48.4 vs. 16.9 ± 6.1) but the mean percentage (± S.E.M.) of useful oocytes was lower (22.4 ± 7.5% vs. 80.2 ± 6.8%; P < 0.01) for small than for medium follicles. The mean number (± S.E.M.) of useful oocytes per donor was significantly (P < 0.01) higher (42.1 ± 16.8 vs. 11.9 ± 4.3) but the mean percentage of matured oocytes was significantly (P < 0.01) lower (48.4 ± 17.8% vs. 79.9 ± 7.9%) for small than for medium follicles. The oocyte population collected from small follicles varied during the oestrous cycle. The mean number (± S.E.M.) of oocytes isolated per donor from small follicles increased during the luteal and decreased during the follicular phase, except for the late follicular phase when it increased again. The mean percentage (± S.E.M.) of useful oocytes did not differ too much during this period, except for the late follicular phase when it decreased significantly (P < 0.01). The mean number (± S.E.M.) of useful oocytes per donor increased during the luteal and decreased during the follicular phase, but the differences were not significant except for the late follicular phase (P < 0.01). Similarly, the mean percentage (± S.E.M.) of matured oocytes increased during the luteal and decreased during the follicular phase, and the differences were significant. Compared with the oocyte population from small follicles, the oocyte population from medium follicles was less variable in the period from the middle luteal to middle follicular phase, when these follicles were present on the ovaries. It can be concluded that the porcine oocyte population changes in terms of quantity, morphological quality and meiotic competence according to the stage of follicular development. The late luteal and early follicular phases appeared to be most productive for oocyte recovery, because more morphologically normal oocytes with greater meiotic competence were collected, as compared with the other stages of the oestrous cycle.

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“…Ultrastructural observations of the zona pellucida of porcine oocytes (Funahashi et al 2000a) have found morphological differences in the zona pellucida of oocytes matured in vitro and in vivo and these authors hypothesized that the differences due to a failure in final maturation may result in incomplete or delayed zona reaction. Polyspermy rate appears to be affected by the donor of oocytes (Marchal et al 2001) and is still higher for in vitro matured oocytes than for ovulated oocytes (Wang et al 1998). Improvement of the quality of porcine IVM oocytes and use of the oocytes in the current IVF system may help to overcome polyspermy.…”

Section: Discussionmentioning

confidence: 99%

Reduction of the incidence of polyspermic penetration into porcine oocytes by pretreatment of fresh spermatozoa with adenosine and a transient co-incubation of the gametes with caffeine

Funahashi¹,

Romar²

2004

Reproduction

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To reduce the incidence of polyspermic penetration, the effects of transient exposure of washed fresh spermatozoa to caffeine in a brief co-culture in vitro fertilization (IVF) system were examined. A pretreatment effect of spermatozoa with adenosine was also examined. When 5 mmol caffeine/l was supplemented during periods of co-culture and additional culture periods until 8 h after insemination, a shortened co-incubation period of gametes (30 denuded oocytes in 100 ml modified Medium 199-suspended spermatozoa at 2.5 3 10 5 sperm/ml) from 30 to 5 min increased the monospermy rate in total mature oocytes examined. The number of spermatozoa binding to the zona surface was significantly lower in oocytes co-cultured for 5 min (33.1 6 2.2) than 8 h (207.6 6 13.7). A limited exposure of gametes to 5 mmol caffeine/l only during a transient co-culture period for 5 or 30 min significantly reduced the mean number of sperm cells that penetrated into the oocyte. Transient exposure of spermatozoa to caffeine for only 5 min increased the percentage of capacitated cells but not acrosome-reacted cells, as compared with a whole exposure treatment. Furthermore, preincubation of spermatozoa with 10 mmol adenosine/l for 90 min increased both the incidence of capacitated cells and the monospermy rate and consequently decreased the number of sperm cells that penetrated into the oocyte. In conclusion, these results have demonstrated that a new transient co-incubation IVF system, in which denuded oocytes are co-cultured with spermatozoa in medium containing caffeine for 5 to 30 min and then continuing the culture in caffeine-free medium, will reduce the incidence of polyspermic penetration. Preincubation of fresh spermatozoa with adenosine before the transient co-incubation IVF can also improve the monospermy rate. Furthermore, asynchrony in the morphology of sperm nuclei in polyspermic oocytes was reduced by the pretreatment with adenosine and a brief exposure to caffeine.

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Meiotic and developmental competence of prepubertal and adult swine oocytes

Cited by 139 publications

References 43 publications

In vitro developmental competence of prepubertal goat oocytes cultured with recombinant activin-A

In vitro developmental competence of prepubertal goat oocytes cultured with recombinant activin-A

Oestrous cycle stage influences the morphology and maturation of porcine oocytes in vitro

Reduction of the incidence of polyspermic penetration into porcine oocytes by pretreatment of fresh spermatozoa with adenosine and a transient co-incubation of the gametes with caffeine

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