Meiotic analysis and FISH with rDNA and rice BAC probes of the Thai KPS 01-01-25 sugarcane cultivar

Thumjamras, S.; Iamtham, Siriluck; Prammanee, S.; Jong, Hans de

doi:10.1007/s00606-015-1264-4

Cited by 8 publications

(8 citation statements)

References 76 publications

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“…Surprisingly, despite this very high level of genome complexity, meiosis of sugarcane cultivars involved mainly bivalent pairing with only a few irregularities found at anaphase I, such as chromosome bridges and laggards (Burner, 1991; Burner and Legendre, 1993; Thumjamras et al ., 2016; Vieira et al ., 2018).…”

Section: Discussionmentioning

confidence: 99%

Sugarcane genome architecture decrypted with chromosome‐specific oligo probes

Piperidis

D’Hont

2020

The Plant Journal

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SUMMARY Sugarcane (Saccharum spp.) is probably the crop with the most complex genome. Modern cultivars (2n = 100–120) are highly polyploids and aneuploids derived from interspecific hybridization between Saccharum officinarum (2n = 80) and Saccharum spontaneum (2n = 40–128). Chromosome‐specific oligonucleotide probes were used in combination with genomic in situ hybridization to analyze the genome architecture of modern cultivars and representatives of their parental species. The results validated a basic chromosome number of x = 10 for S. officinarum. In S. spontaneum, rearrangements occurred from a basic chromosome of x = 10, probably in the Northern part of India, in two steps leading to x = 9 and then x = 8. Each step involved three chromosomes that were rearranged into two. Further polyploidization led to the wide geographical extension of clones with x = 8. We showed that the S. spontaneum contribution to modern cultivars originated from cytotypes with x = 8 and varied in proportion between cultivars (13–20%). Modern cultivars had mainly 12 copies for each of the first four basic chromosomes, and a more variable number for those basic chromosomes whose structure differs between the two parental species. One−four of these copies corresponded to entire S. spontaneum chromosomes or interspecific recombinant chromosomes. In addition, a few inter‐chromosome translocations were revealed. The new information and cytogenetic tools described in this study substantially improve our understanding of the extreme level of complexity of modern sugarcane cultivar genomes.

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Section: Discussionmentioning

confidence: 99%

Sugarcane genome architecture decrypted with chromosome‐specific oligo probes

Piperidis

D’Hont

2020

The Plant Journal

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“…For example, established autotetraploids of Arabidopsis arenosa show a reduction in the number of crossovers per chromosome compared to the diploid ancestor (Bomblies et al, 2016). Diakinesis cells of the Thai KPS 01-01-25 sugarcane exhibited not only true chiasma bonds between homologs/homeologs, but also secondary chromosome associations resulting from loose bivalents in the absence of chiasmata (Thumjamras et al, 2016). This reduction in chiasma frequency may also indicate how sensitive the structural meiotic components are to disturbance, and could be a direct consequence of meiotic adaptation to genomic challenges (Bomblies et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Revisiting Meiosis in Sugarcane: Chromosomal Irregularities and the Prevalence of Bivalent Configurations

et al. 2018

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Traditional sugarcane cultivars (Saccharum officinarum) proved highly susceptible to diseases, and this led breeders to progress to interspecific crosses resulting in disease resistance. A backcrossing program to S. officinarum was then required to boost sucrose content. Clonal selection across generations and incorporation of other germplasm into cultivated backgrounds established the (narrow) genetic base of modern cultivars (Saccharum spp.), which have a man-made genome. The genome complexity has inspired several molecular studies that have elucidated aspects of sugarcane genome constitution, architecture, and cytogenetics. However, there is a critical shortage of information on chromosome behavior throughout meiosis in modern cultivars. In this study, we examined the microsporogenesis of a contemporary variety, providing a detailed analysis of the meiotic process and chromosome association at diakinesis, using FISH with centromeric probes. Chromosomal abnormalities were documented by examining high quality preparations of pollen mother cells (700 in total). Approximately 70% of the cells showed abnormalities, such as metaphase chromosomes not lined up at the plate, lagging chromosomes and chromosomal bridges, and tetrad cells with micronuclei. Some dyads with asynchronous behavior were also observed. Due to the hybrid composition of the sugarcane genome, we suggest that bivalent incomplete pairing may occur in the first prophase leading to univalency. The presence of rod bivalents showing the lagging tendency is consistent with a reduction in chiasma frequency. Finally, the presence of chromatin bridges indicates the indirect occurrence of chromosomal inversions, although chromosome fragments were not clearly recognized. Possible reasons for such meiotic abnormalities and the large prevalence of bivalent formation are discussed.

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“…Traditional cytogenetic markers, such as repetitive sequences and BAC clones, only allowed identification of a few chromosomes in sugarcane (D'Hont et al ., 1998; Garsmeur et al ., 2011; Dong et al ., 2018). In addition, probes based on repetitive DNA sequences often generate a variable number of FISH foci in different cells (Thumjamras et al ., 2016) and many BAC probes produce strong background signals in sugarcane (Dong et al ., 2018). Oligonucleotide‐based FISH probes have recently been developed for sugarcane chromosome research.…”

Section: Discussionmentioning

confidence: 99%

Chromosome‐specific painting unveils chromosomal fusions and distinct allopolyploid species in the Saccharum complex

et al. 2021

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Karyotypes provide key cytogenetic information on the phylogenetic relationships and evolutionary origins in related eukaryotic species. Despite our knowledge of the chromosome numbers of sugarcane and its wild relatives, the chromosome composition and evolution among the species in the Saccharum complex have been elusive owing to the complex polyploidy and the large numbers of chromosomes of these species.Oligonucleotide-based chromosome painting has become a powerful tool of cytogenetic studies especially for plant species with large numbers of chromosomes. We developed oligobased chromosome painting probes for all 10 chromosomes in Saccharum officinarum (2n = 8x = 80). The 10 painting probes generated robust fluorescence in situ hybridization signals in all plant species within the Saccharum complex, including species in the genera Saccharum, Miscanthus, Narenga and Erianthus.We conducted comparative chromosome analysis using the same set of probes among species from four different genera within the Saccharum complex. Excitingly, we discovered several novel cytotypes and chromosome rearrangements in these species.We discovered that fusion from two different chromosomes is a common type of chromosome rearrangement associated with the species in the Saccharum complex. Such fusion events changed the basic chromosome number and resulted in distinct allopolyploids in the Saccharum complex.

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Meiotic analysis and FISH with rDNA and rice BAC probes of the Thai KPS 01-01-25 sugarcane cultivar

Cited by 8 publications

References 76 publications

Sugarcane genome architecture decrypted with chromosome‐specific oligo probes

Sugarcane genome architecture decrypted with chromosome‐specific oligo probes

Revisiting Meiosis in Sugarcane: Chromosomal Irregularities and the Prevalence of Bivalent Configurations

Chromosome‐specific painting unveils chromosomal fusions and distinct allopolyploid species in the Saccharum complex

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