Meiotic abnormalities affect genetic constitution and pollen viability in dicots from Indian cold deserts

Kaur, Dalbir; Singhal, Vijay Kumar

doi:10.1186/s12870-018-1596-7

Cited by 30 publications

(15 citation statements)

References 37 publications

(37 reference statements)

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“…The above mentioned meiotic abnormalities were detected in many plant species [ 50 , 51 ]. They are considered to be related to some genetic [ 52 , 53 ] and/or environmental [ 54 , 55 , 56 ] factors. Such chromosomal disorders can result in sterility of gametes and low percentage of pollen viability [ 57 ].…”

Section: Discussionmentioning

confidence: 99%

Agro-Morphological, Microanatomical and Molecular Cytogenetic Characterization of the Medicinal Plant Chelidonium majus L.

Samatadze

Yurkevich

Хазиева

et al. 2020

Plants

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Chelidonium majus L. is a medicinal plant well-known as a valuable source of isoquinoline alkaloids, which has a variety of pharmacological properties including anti-viral and anti-bacterial effects. However, considerable intraspecific bio-morphological variability in C. majus complicates raw material identification and verification. For the first time, we have brought into cultivation five populations of C. majus subsp. majus originated from different regions, and performed their agro-morphological, microanatomical and molecular cytogenetic characterization. All examined populations produced high seed (18.6–19.9 kg/ha) and raw material (0.84–1.08 t/ha) yields; total alkaloid contents were within 0.30–0.38%. Nevertheless, significant differences in plant morphology and yield-contributing traits were observed. The performed microanatomical analysis of leaves and flowers in double- and normal-flowered plants revealed micro-diagnostic features (including tissue topography, types of stomata, laticifers, structure of leaf mesophyll, hairs, sepals and petals) important for identification of C. majus raw materials. The analysis of chromosome morphology, DAPI-banding patterns, FISH mapping of 45S and 5S rDNA and also chromosome behavior in meiosis allowed us to identify for the first time all chromosomes in karyotypes and confirm relative genotype stability of the studied plants. Our findings indicate that the examined C. majus populations can be used in further breeding programs.

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Section: Discussionmentioning

confidence: 99%

Agro-Morphological, Microanatomical and Molecular Cytogenetic Characterization of the Medicinal Plant Chelidonium majus L.

Samatadze

Yurkevich

Хазиева

et al. 2020

Plants

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“…The INM frequency estimated by means of squashed preparations combined with LM is highly varied among species and even within a species if this parameter is estimated by different authors. It can vary from <1 to 80-100% of male meiocytes in an anther (Li et al, 2009;Reis et al, 2016;Kaur and Singhal, 2019). Such high variation of the numbers makes them doubtful.…”

Section: Inm In Tobacco Meiosis Has Been Greatly Underestimatedmentioning

confidence: 99%

Serial Block-Face Scanning Electron Microscopy Reveals That Intercellular Nuclear Migration Occurs in Most Normal Tobacco Male Meiocytes

et al. 2021

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Serial block-face scanning electron microscopy (SBF-SEM) was used here to study tobacco male meiosis. Three-dimensional ultrastructural analyses revealed that intercellular nuclear migration (INM) occurs in 90–100% of tobacco meiocytes. At the very beginning of meiosis, every meiocyte connected with neighboring cells by more than 100 channels was capable of INM. At leptotene and zygotene, the nucleus in most tobacco meiocytes approached the cell wall and formed nuclear protuberances (NPs) that crossed the cell wall through the channels and extended into the cytoplasm of a neighboring cell. The separation of NPs from the migrating nuclei and micronuclei formation were not observed. In some cases, the NPs and nuclei of neighboring cells appeared apposed to each other, and the gap between their nuclear membranes became invisible. At pachytene, NPs retracted into their own cells. After that, the INM stopped. We consider INM a normal part of tobacco meiosis, but the reason for such behavior of nuclei is unclear. The results obtained by SBF-SEM suggest that there are still many unexplored features of plant meiosis hidden by limitations of common types of microscopy and that SBF-SEM can turn over a new leaf in plant meiosis research.

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“…They suggest that the irregular chromosome pairing observed in the BC1 plants is one of the main reasons of the problem of fertility observed. Usually in plant species with normal genomes, homologous chromosomes tend to have high matching rate unlike the homoeologous chromosomes in hybrid plants derived from interspecific crosses (Kaur and Singhal 2019). Actually, an essential event in meiosis is the chromosome pairing, not only for the occurrence of recombination but also for the correct chromosome segregation.…”

Section: Discussionmentioning

confidence: 99%

Relationship between meiotic behaviour and fertility in backcross-1 derivatives of the [(Gossypium hirsutum × G. thurberi)2 × G. longicalyx] trispecies hybrid

Konan

Mergeai

2020

CCG

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Wild cotton species are an important source of desirable genes for genetic improvement of cultivated cotton Gossypium hirsutum Linnaeus, 1763. For the success of such an improvement, chromosome pairings and recombinations in hybrids are fundamental. The wild African species G. longicalyx Hutchinson & Lee, 1958 could be used as donor of the desirable trait of fiber fineness. Twelve BC1 plants obtained from the backcrossing of [(G. hirsutum × G. thurberi Todaro, 1877)2 × G. longicalyx] (AhDhD1F1, 2n = 4x = 52) trispecies hybrid (HTL) by G. hirsutum (cv. C2) (AhAhDhDh, 2n = 4x = 52) were investigated for meiotic behaviour and plant fertility. Their chromosome associations varied as follows: (2.5 to 11.5) I + (17 to 22) II + (0.31 to 1.93) III + (0.09 to 1.93) IV + (0 to 0.07) V + (0 to 0.14) VI. Their pollen fertility ranged from 4.67 to 32.10 %. Only four BC1 plants produced a few seeds through self-pollination. The remaining BC1 were totally self-sterile and usually presented the highest number of univalents. All BC1 materials produced BC2 seeds (0.44 to 6.50 seeds per backcross) with the number of seeds negatively correlated with the number of univalents (R2 = 0.45, P < 0.05). Most BC1 plants gave significantly finer fiber compared to the cultivated G. hirsutum. SSR markers showed a segregation of wild alleles among the backcross derivatives and Genomic in situ hybridization (GISH) revealed presence of entire chromosomes of G. longicalyx as well as recombinant chromosomes in the backcross derivatives. The significance and details of these results are presented and the prospects of successfully exploiting these plant materials are discussed.

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Meiotic abnormalities affect genetic constitution and pollen viability in dicots from Indian cold deserts

Cited by 30 publications

References 37 publications

Agro-Morphological, Microanatomical and Molecular Cytogenetic Characterization of the Medicinal Plant Chelidonium majus L.

Agro-Morphological, Microanatomical and Molecular Cytogenetic Characterization of the Medicinal Plant Chelidonium majus L.

Serial Block-Face Scanning Electron Microscopy Reveals That Intercellular Nuclear Migration Occurs in Most Normal Tobacco Male Meiocytes

Relationship between meiotic behaviour and fertility in backcross-1 derivatives of the [(Gossypium hirsutum × G. thurberi)2 × G. longicalyx] trispecies hybrid

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