Meiofauna Techniques

Somerfield, Paul J.; Warwick, R. M.; Moens, Tom

doi:10.1002/9780470995129.ch6

Cited by 54 publications

(23 citation statements)

References 112 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the laboratory, extraction of nematodes and mounting methods followed Somerfield and Warwick (1996) and Somerfield et al (2005). Briefly, cores were washed through a 500-μm sieve overlying a 63-μm sieve to remove formalin and larger sediment particles, and the fauna extracted by flotation using Ludox-TM with a specific gravity of 1.15.…”

Section: Study Site and Samples Collectionmentioning

confidence: 99%

The response of nematode assemblages to intensive mussel farming in coastal sediments (Southern Brazil)

Netto

Valgas

2009

Environ Monit Assess

View full text Add to dashboard Cite

The impact of mussel farms on the benthic environment was investigated using free-living nematode assemblages as indicators of environmental condition of the Enseada do Brito, a shallow embayment in South Brazil. The studied area is located at the State of Santa Catarina, responsible for 90% of the production of mussel farmed in the country. Samplings were carried out quarterly, from July 2004 to May 2005, below mussel ropes and at control sites located 300 m apart from the longlines. The results suggested that the mussel culture changed ecological functioning of the benthic environment at studied coastal embayment. The intense organic loads due to the biodeposition of the mussel farms, which had been in production for more than 10 years, have turned fine and well-sorted sand bottoms into a muddy deposit with significantly higher total organic content than the control. The number of nematode genera, diversity, dominance and trophic diversity were significantly lower under the mussel farms than at the control sites. The mussel farms biodeposition had also affected the temporal variability of nematode assemblages, which was detected only for samples at the control sites. The low depth and environmental energy, together with the age of the farms, were probably responsible for the observed changes in the nematode assemblages.

show abstract

Section: Study Site and Samples Collectionmentioning

confidence: 99%

The response of nematode assemblages to intensive mussel farming in coastal sediments (Southern Brazil)

Netto

Valgas

2009

Environ Monit Assess

View full text Add to dashboard Cite

show abstract

“…The RB is a typical bulk stain which adheres to (cytoplasmatic) proteins and is applied into formalin-fixed samples (Higgins and Thiel, 1988;Somerfield et al, 2005). As a stain it has various advantages (cheap, simple to apply, animals are easily visible under light microscope), but on the negative side it is a nonvital stain that stains proteins regardless if the cell/animal is dead or alive (Bernhard et al, 2006).…”

Section: Grego Et Al: Ctg Labelling Vs Rose Bengal Stainingmentioning

confidence: 99%

“…Rose bengal (4,5,6,7-tetrachloro-2 ,4 ,5 ,7 -tetraiodofluorescein disodium salt) is a widely used bulk stain (Higgins and Thiel, 1988;Somerfield et al, 2005) that adheres to (cytoplasmatic) proteins, regardless of whether the cell/animal is dead or alive (Bernhard et al, 2006). It colours different meiofauna organisms differently (Giere, 2009) and has the advantage of being cheap and easy to apply, and only a light microscope is required; on the negative side it is a nonvital stain.…”

Section: Labelling/staining Protocolmentioning

confidence: 99%

“…The containers were incubated at in situ temperature in the dark for 12 h. Afterwards, samples were fixed in 4 % borax-buffered (5 g L −1 ) formalin. For RB staining, the RB was applied as 1 g L −1 solution (in 10 % formalin) into fixed samples (4 % borax-buffered formalin) to yield a final 1 % solution (Higgins and Thiel, 1988;Somerfield et al, 2005).…”

Section: Labelling/staining Protocolmentioning

confidence: 99%

See 1 more Smart Citation

CellTracker Green labelling vs. rose bengal staining: CTG wins by points in distinguishing living from dead anoxia-impacted copepods and nematodes

et al. 2013

View full text Add to dashboard Cite

Abstract. Hypoxia and anoxia have become a key threat to shallow coastal seas. Much is known about their impact on macrofauna, less on meiofauna. In an attempt to shed more light on the latter group, in particular from a process-oriented view, we experimentally induced short-term anoxia (1 week) in the northern Adriatic Sea (Mediterranean) and examined the two most abundant meiofauna taxa – harpacticoid copepods and nematodes. Both taxa also represent different ends of the tolerance spectrum, with copepods being the most sensitive and nematodes among the most tolerant. We compared two methods: CellTracker Green (CTG) – new labelling approach for meiofauna – with the traditional rose bengal (RB) staining method. CTG binds to active enzymes and therefore colours live organisms only. The two methods show considerable differences in the number of living and dead individuals of both meiofauna taxa. Generally, RB will stain dead but not yet decomposed copepods and nematodes equally as it does live ones. Specifically, RB significantly overestimated the number of living copepods in all sediment layers in anoxic samples, but not in any normoxic samples. In contrast, for nematodes, the methods did not show such a clear difference between anoxia and normoxia. RB overestimated the number of living nematodes in the top sediment layer of normoxic samples, which implies an overestimation of the overall live nematofauna. For monitoring and biodiversity studies, the RB method might be sufficient, but for more precise quantification of community degradation, especially after an oxygen depletion event, CTG labelling is a better tool. Moreover, it clearly highlights the surviving species within the copepod or nematode community. As already accepted for foraminiferal research, we demonstrate that the CTG labelling is also valid for other meiofauna groups.

show abstract

“…The subsamples were wetsieved using 1 000 µm and 63 µm sieves [26] . The residue on the 63 µm sieve was then carefully inspected under a dissecting microscope.…”

Section: Sampling Of Foraminifera Assemblages and Physicochemical Varmentioning

confidence: 99%

The distribution of benthic foraminiferal assemblages in the north-west coastal region of Malacca Straits, Malaysia

Yahya

Shuib²,

Minhat³

et al. 2014

JCLM

View full text Add to dashboard Cite

Meiofauna Techniques

Cited by 54 publications

References 112 publications

The response of nematode assemblages to intensive mussel farming in coastal sediments (Southern Brazil)

The response of nematode assemblages to intensive mussel farming in coastal sediments (Southern Brazil)

CellTracker Green labelling vs. rose bengal staining: CTG wins by points in distinguishing living from dead anoxia-impacted copepods and nematodes

The distribution of benthic foraminiferal assemblages in the north-west coastal region of Malacca Straits, Malaysia

Contact Info

Product

Resources

About