Megaloneurite, a giant neurite of vasoactive intestinal peptide and nitric oxide synthase in the aged dog and identification by human sacral spinal cord

Li, Yinhua; Hou, Wei; Jia, Yunge; Rao, Chenxu; Wei, Zichun; Xu, Xiuhua; Li, Hang; Li, Fuhong; Wang, Xinghang; Zhang, Tianyi; Sun, Jui-Hung; Tan, Huibing

doi:10.1101/726893

Cited by 2 publications

(7 citation statements)

References 108 publications

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“…The case study was completed in a 10-year-old dog accompanied by spinal disc herniation. We have already reported the megaloneurite in the sacral spinal cord of aged dog [9,14]. In this study, the N-d positive megaloneurite occurred conformally in the sacral spinal cord.…”

Section: Discussionsupporting

confidence: 62%

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Novel Morphological Alteration and Neural Pathway of NADPH Diaphorase Positivity in the Spinal Cord with Disc Herniation of Aged Dog: A Case Report

Li¹,

Jia²,

Hou³

et al. 2020

Preprint

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Neuronal lesion or injury is a traditional approach to investigate neural circuit. Is any new neural pathway or new neurodegeneration related central nerve system injury? Spinal disc herniation can cause the spinal cord injury. However, the histological examination is still lack. It happened that a case of spinal disc herniation of a 10-year old dog was examined with NADPH diaphorase (N-d) histology. We did not find the N-d neurodegenerative aberrant in the tissue of the mid-rostral lumber segment besides the metamorphoses by the compression of the disc herniation. However, the severe neuropathological changes majorly occurred in the lumbosacral spinal cord. We found more diverse neurodegenerative alterations: the aging-related N-d body (ANB), megaloneurite and N-d homogeneous formazan globule in the lumbosacral spinal cord. We also found that a new circuit pathway (intermedial collateral pathway) showed by a megaloneurite between the lateral collateral pathway and the medial collateral pathway. The enormous notch caused by spinal disc herniation located at the mid-rostral lumber segments. The aging-related neurodegeneration occurred the specific lumbosacral segments. The homogeneous formazan globule was round or oval homogeneous N-d positivity which distributed in the gray matter and dorsal column. In the medulla oblongata, ANBs were revealed in the gracile nucleus, nucleus reticularis lateralis (ventrolateral spinal trigeminal nucleus) and middle of the spinal trigeminal nucleus.

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Section: Discussionsupporting

confidence: 62%

“…The aging-related N-d neurodegeneration occurred normally in the lumbosacral spinal cord of the aged dog [9,14]. In the thoracic segment rostral to the disc herniation, no megaloneurite and homogeneous formazan globules were detected besides occasional small size ANBs (Figure 7).…”

Section: Resultsmentioning

confidence: 98%

Novel Morphological Alteration and Neural Pathway of NADPH Diaphorase Positivity in the Spinal Cord with Disc Herniation of Aged Dog: A Case Report

Li¹,

Jia²,

Hou³

et al. 2020

Preprint

Self Cite

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“…Next, we examined the same segment of the sacral spinal cord in aged dog. As the previous investigation, the ANBs and megaloneurites were detected in the sacral spinal cord in aged dog [24,29]. The caudal sacral spinal cord occurred relative a smaller number of the megaloneurites than the rostral sacral.…”

Section: Resultssupporting

confidence: 74%

“…The distribution of the N-d fibers kept consistent with the VIPergic fibers distributed in the around the central canal and the anterior fissure. In our previous study, we also find that the VIP-ergic fibers distribute around the central canal [29]. Meanwhile, N-d megaloneurites and some fibers co-localize with VIP [22,54].…”

Section: Discussionmentioning

confidence: 64%

“…Meanwhile, N-d megaloneurites and some fibers co-localize with VIP [22,54]. Some VIP positive fibers and megaloneurites distribute toward to the anterior commissure [29]. In the middle of the central canal, there is a thread-like structure named Reissner's fiber extending from the subcommissural organ to the entire central canal in the spinal cord [55].…”

Section: Discussionmentioning

confidence: 99%

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A pathway of NADPH diaphorase positivity between central canal and pial surface at anterior fissure in spinal cord: Supra fissure area with hypothesis configuring from dog, rat, monkey and pigeon

Jia

Hou

et al. 2020

Preprint

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The spinal cord is a cylinder structure in the vertebra and thought a simplified with the gray matter and white matter. Rexed lamination for the gray matter and regional sub-division for whiter matter are completely termed to date. Anterior commissure locates between the central canal and the anterior median fissure. However, some experimental data may still confront with new confined anatomical interpretation. By using NADPH diaphorase [N-d] enzyme histology, we found a vertical oriented neuronal pathway between the central canal and the anterior median fissure in the sacral spinal cord of young adult and aged dog. We used a term "supra fissure area" [SFA] to illustrate the region which consisted of the gray commissure and anterior white commissure. The N-d pathway was notably observable in aged animals. The vertical neurites revealed the cerebrospinal fluid [CSF] contacting neurites between the anterior median fissure and the central canal. We further examined the monkey, rat and pigeon in the region for better understanding of the structure and potential function. The neurodegeneration of N-d dystrophy was detected in the [SFA] in the thoracic spinal cord of the aged monkey. N-d positive fibers were detected in anterior fissure of the rat spinal cord. N-d fibrous structures were also detected in the pigeon spinal cord. These results suggested a new pathway of CSF contacting neurons and the neuronal communications about the central canal.

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Megaloneurite, a giant neurite of vasoactive intestinal peptide and nitric oxide synthase in the aged dog and identification by human sacral spinal cord

Cited by 2 publications

References 108 publications

Novel Morphological Alteration and Neural Pathway of NADPH Diaphorase Positivity in the Spinal Cord with Disc Herniation of Aged Dog: A Case Report

Novel Morphological Alteration and Neural Pathway of NADPH Diaphorase Positivity in the Spinal Cord with Disc Herniation of Aged Dog: A Case Report

A pathway of NADPH diaphorase positivity between central canal and pial surface at anterior fissure in spinal cord: Supra fissure area with hypothesis configuring from dog, rat, monkey and pigeon

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