Meeting report of the International Consortium of Stem Cell Networks’ Workshop Towards Clinical Trials Using Stem Cells for Amyotrophic Lateral Sclerosis/Motor Neuron Disease

Chaddah, M. R.; Dickie, Brian; Lyall, Drew; Marshall, Caroline; Sykes, J Ben; Bruijn, Lucie

doi:10.3109/17482968.2011.590992

Cited by 10 publications

(3 citation statements)

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“…At present, more than 234 hPSC lines have been registered at the National Institutes of Health (NIH), which are eligible for use in federally funded research. Numerous discussions have taken place in recent years about standards for derivation, registries, characterization, storage, banking, distribution, and engineering of hPSCs for both research and therapy (Adewumi et al, 2007; Akopian et al, 2010; Andrews et al, 2009; Borstlap et al, 2010; Chaddah et al, 2011; Crook et al, 2010; Panchision, 2013; Rao, 2013; Stacey et al, 2013; Turner et al, 2013). The subsequent resources and platforms that have emerged have opened the door to therapeutic application for regenerative medicine and for biomedical research.…”

Section: Introductionmentioning

confidence: 99%

Human Pluripotent Stem Cell Culture: Considerations for Maintenance, Expansion, and Therapeutics

et al. 2014

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Summary Human pluripotent stem cells (hPSCs) provide powerful resources for application in regenerative medicine and pharmaceutical development. In the past decade, various methods have been developed for large-scale hPSC culture that rely on combined use of multiple growth components, including media containing various growth factors, extracellular matrices, three-dimensional environmental (3D) cues and modes of multicellular association. In this review, we dissect these growth components by comparing cell culture methods and identifying the benefits and pitfalls associated with each one. We further provide criteria, considerations, and suggestions to achieve optimal cell growth for hPSC expansion, differentiation, and use in future therapeutic applications.

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Section: Introductionmentioning

confidence: 99%

Human Pluripotent Stem Cell Culture: Considerations for Maintenance, Expansion, and Therapeutics

et al. 2014

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“…The induced cells produce and release high amounts of NTFs, such as BDNF and GDNF (Sadan et al, 2008;Dadon-Nachum et al, 2011) which protect cultured MNs. Also MSC-NTF derived xeno-free growth media, a technology licensed to Brainstorm, markedly increase the expansion potential of MSCs (Chaddah et al, 2011). Transplantation studies in mice and rats thus far have also proved successful.…”

Section: Mesenchymal Stem Cellsmentioning

confidence: 99%

Transplantation of mesenchymal stem cells in ALS

Mazzini

Vercelli

Ferrero³

et al. 2012

Functional Neural Transplantation III - Primary and Stem Cell Therapies for Brain Repair, Part II

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“…In clinical treatment of neuronal medicine, various types of neuronal cells are reconstructed or transplanted into patients who suffer severe neuronal diseases or injuries such as Amyotrophic Lateral Sclerosis (ALS) [1], Parkinson's disease [2,3] and spinal cord injury [4][5][6][7][8]. In the case of spinal cord injury, for example, reconstruction of the damaged neuronal cells colony might be carried out first if the damage is not so severe.…”

Section: Introductionmentioning

confidence: 99%

A Novel In Vitro Simulator to Investigate Promotion of Reconstruction of Damaged Neuronal Cell Colony Differentiated from iPS Cells with the Aid of Micro Dynamic Stimulation

et al. 2021

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Neuronal cells are equipped with the function of a sensor that senses stimulation and elongates neurites to connect nearby neuronal cells in forming a neuronal network, as they are generally said to be hard to recover from physical damage, such as in the case of a spinal cord injury. Therefore, in this study, a novel in vitro simulator in which micro dynamic stimulations are applied to a damaged neuronal cell colony artificially is proposed to investigate the possibility of promoting the reconstruction of damaged neuronal cells on a colony basis. A neuronal cell colony differentiated from iPS cells is physically damaged by cutting off treatment, and micro dynamic stimulations are applied to the colony by utilizing a developed mini-vibration table system. NeuroFluor NeuO is used to establish a method for fluorescent staining of the living neuronal cells, and morphologies of the reconstructing neurons are analysed, revealing a relationship between the stimulation and the reconstructing process of the damaged neurons. It is found that significant differences are observed in the reconstructing efficiency between the statically cultured damaged neuronal cell colony and the dynamically stimulated one. The results suggest that applying appropriate micro dynamic stimulations is a promising approach to promote the reconstruction of a damaged neuronal cell colony.

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Meeting report of the International Consortium of Stem Cell Networks’ Workshop Towards Clinical Trials Using Stem Cells for Amyotrophic Lateral Sclerosis/Motor Neuron Disease

Cited by 10 publications

References 0 publications

Human Pluripotent Stem Cell Culture: Considerations for Maintenance, Expansion, and Therapeutics

Human Pluripotent Stem Cell Culture: Considerations for Maintenance, Expansion, and Therapeutics

Transplantation of mesenchymal stem cells in ALS

A Novel In Vitro Simulator to Investigate Promotion of Reconstruction of Damaged Neuronal Cell Colony Differentiated from iPS Cells with the Aid of Micro Dynamic Stimulation

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