Medullary astrocytes mediate irregular breathing patterns generation in chronic heart failure through purinergic P2X7 receptor signalling

Toledo, Camilo; Díaz, Hugo S.; Schwarz, Karla G.; Pereyra, Katherin V.; Heras, Alexandra Las; Ríos-Gallardo, Angélica P.; Andrade, David C.; Moreira, Thiago S.; Takakura, Ana C.; Marcus, Noah J.; Río, Rodrigo Del

doi:10.1016/j.ebiom.2022.104044

Cited by 3 publications

(9 citation statements)

References 57 publications

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“…Augmented central chemoreflex drive has been associated with the development of breathing disorders and irregular breathing patterns [ 3 ]. We recently demonstrated that the activation of RTN chemoreceptor neurons induces breathing disorders in healthy animals [ 40 ]. However, the impact of elevated ROS levels in the RTN on the occurrence of breathing disorders in healthy animals has not been previously investigated.…”

Section: Discussionmentioning

confidence: 99%

“…Resting breathing (RB) and chemoreflex function were recorded through whole-body plethysmography (EMKA Technologies, France) in freely moving mice. Over the course of 3 h, RB was recorded while mice breathed room air in the plethysmograph [ 11 , 12 , 38 , 40 ]. All the experiments were performed between 10:00 and 16:00 h. Animals were habituated to a whole-body plethysmography chamber for two consecutive days prior to recording.…”

Section: Methodsmentioning

confidence: 99%

“…On the day of the experiment, a minimum of 2 h was allowed for the mice to acclimatize to the recording chamber. Plethysmograph was flushed at 0.75 L/min [ 40 ]. Chamber temperature (23.5 ± 0.1 °C), atmospheric pressure and relative humidity (42.0 ± 2.0 %) were monitored and stable during recordings.…”

Section: Methodsmentioning

confidence: 99%

“…Chamber temperature (23.5 ± 0.1 °C), atmospheric pressure and relative humidity (42.0 ± 2.0 %) were monitored and stable during recordings. Ventilatory parameters as tidal volume (V T ), respiratory frequency (R f ), minute ventilation (V E : V T x R f ), inspiratory time (T i ), expiratory time (T e ), total respiratory time (T TOT ), peak expiratory flow (PeF), peak inspiratory flow (PiF) were determined using ecgAUTO software (EMKA Technologies, France) [ 11 , 12 , 38 , 40 ].…”

Section: Methodsmentioning

confidence: 99%

“…In addition to RTN chemoreceptor neurons, RTN astrocytes have gained attention for their role in regulating breathing through the release of various gliotransmitters that modulate RTN chemoreceptor neurons [ 19 , 37 , 40 ]. Studies from our laboratory have demonstrated that decreased expression of Glial fibrillary acidic protein (GFAP) in the RTN, indicative of reduced activity of RTN astrocytes, lead to RTN-mediated breathing disorders [ 40 ]. This suggests that RTN astrocytes contribute to the hyper-responsiveness of RTN chemoreceptor neurons.…”

Section: Introductionmentioning

confidence: 99%

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Superoxide dismutase 2 deficiency is associated with enhanced central chemoreception in mice: Implications for breathing regulation

Díaz-Jara,

Pereyra,

Vicencio

et al. 2024

Redox Biology

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Superoxide dismutase 2 deficiency is associated with enhanced central chemoreception in mice: Implications for breathing regulation

Díaz-Jara,

Pereyra,

Vicencio

et al. 2024

Redox Biology

Self Cite

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Sleep dysregulation in sympathetic-mediated diseases: implications for disease progression

Olivares

Toledo

Ortolani

et al. 2022

Sleep

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The autonomic nervous system (ANS) play an important role in the coordination of several physiological functions including sleep/wake process. Significant changes in ANS activity occur during wake-to-sleep transition maintaining the adequate cardiorespiratory regulation and brain activity. Since sleep is a complex homeostatic function, partly regulated by the ANS, it is not surprising that sleep disruption trigger and/or evidence symptoms of ANS impairment. Indeed, several studies suggest a bidirectional relationship between impaired ANS function (i.e., enhanced sympathetic drive), and the emergence/development of sleep disorders. Furthermore, several epidemiological studies described a strong association between sympathetic-mediated diseases and the development and maintenance of sleep disorders resulting in a vicious cycle with adverse outcomes and increased mortality risk. However, which and how the sleep/wake control and ANS circuitry becomes affected during the progression of ANS-related diseases remains poorly understood. Thus, understanding the physiological mechanisms underpinning sleep/wake-dependent sympathetic modulation could provide insights into diseases involving autonomic dysfunction. The purpose of this review is to explore potential neural mechanisms involved in both the onset/maintenance of sympathetic-mediated diseases (Rett syndrome, congenital central hypoventilation syndrome, obstructive sleep apnoea, type 2 diabetes, obesity, heart failure, hypertension and neurodegenerative diseases) and their plausible contribution to the generation of sleep disorders in order to review evidence that may serve to establish a causal link between sleep disorders and heightened sympathetic activity.

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Neonatal Chlamydia muridarum respiratory infection causes neuroinflammation within the brainstem during the early postnatal period

Hedley,

Gomez,

Kecelioglu

et al. 2024

J Neuroinflammation

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Respiratory infections are one of the most common causes of illness and morbidity in neonates worldwide. In the acute phase infections are known to cause wide-spread peripheral inflammation. However, the inflammatory consequences to the critical neural control centres for respiration have not been explored. Utilising a well characterised model of neonatal respiratory infection, we investigated acute responses within the medulla oblongata which contains key respiratory regions. Neonatal mice were intranasally inoculated within 24 h of birth, with either Chlamydia muridarum or sham-infected, and tissue collected on postnatal day 15, the peak of peripheral inflammation. A key finding of this study is that, while the periphery appeared to show no sex-specific effects of a neonatal respiratory infection, sex had a significant impact on the inflammatory response of the medulla oblongata. There was a distinct sex-specific response in the medulla coincident with peak of peripheral inflammation, with females demonstrating an upregulation of anti-inflammatory cytokines and males showing very few changes. Microglia also demonstrated sex-specificity with the morphology of females and males differing based upon the nuclei. Astrocytes showed limited changes during the acute response to neonatal infection. These data highlight the strong sex-specific impact of a respiratory infection can have on the medulla in the acute inflammatory phase.

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Medullary astrocytes mediate irregular breathing patterns generation in chronic heart failure through purinergic P2X7 receptor signalling

Cited by 3 publications

References 57 publications

Superoxide dismutase 2 deficiency is associated with enhanced central chemoreception in mice: Implications for breathing regulation

Superoxide dismutase 2 deficiency is associated with enhanced central chemoreception in mice: Implications for breathing regulation

Sleep dysregulation in sympathetic-mediated diseases: implications for disease progression

Neonatal Chlamydia muridarum respiratory infection causes neuroinflammation within the brainstem during the early postnatal period

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