Medium optimization of beta-glucanase production by Bacillus subtilis SAHA 32.6 used as biological control of oil palm pathogen

Dewi, Rike Tri Kumala; Mubarik, Nisa Rachmania; Suhartono, Maggy Thenawijaya

doi:10.9755/ejfa.2015-05-195

Cited by 28 publications

(26 citation statements)

References 29 publications

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“…Therefore, it can be concluded that the suitable incubation time was established at 52 and 56 hrs for BG15 and BG21, respectively. Compared to previous research by Dewi et al (2016) reported that the strain B. subtilis SAHA 32.6 produced the highest β-glucanase activity in the medium containing 1% β-glucan after 12 hrs of incubation, the culture time of strain BG15 and BG21 was significantly longer.…”

Section: Effect Of Incubation Time On β-Glucanase Production Ability contrasting

confidence: 77%

Selection of Bacillus subtilis strains capable of producing β-glucanase supporting the hydrolysis of yeast cell walls

Kien

Linh

Hạnh

et al. 2020

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The selection of B. subtilis strains was carried out with 15 strains from the collections of the Vietnam National University of Agriculture and the University of Economic and Technical Industries, Hanoi, Vietnam. To investigate the specific ability of β-glucanase in supporting the hydrolysis of beer yeast cells, CMC substrates in enzyme-activated test media of traditional methods was replaced by yeast cell walls in this study. The B. subtilis strains were activated on Nutrient Broth culture and then transplanted into MT3 culture for producing β-glucanase. Optical density (OD600nm) measurement was used to estimate the bacterial density. The β-glucanase activity formed by bacteria cells free supernatant was quantified by agar diffusion method on the enzyme-activated test media MT4. Two B. Subtilis strains , BG21 and BG15, were selected based on their largest clear-zones on agar plates. By modifying the values of the affecting factors and keeping the remaining influencing factors unchanged, it was determined that the B. subtilis BG21 and BG15 strains produced the highest biomass at the conditions of the culture time of 24 and 28 h, at pH 7.0, and at 37oC, respectively; furthermore, the highest activity of β-glucanase of the two strains BG21 and BG15 was exhibited at the culture time of 52 and 56 h, at pH 7.0, and at 37oC, respectively.Practical applicationsBacillus subtilis strains with the highest β-glucanase producing ability will be used for the production of biological products containing B. subtilis and β-glucanase which supports the hydrolysis of the beer yeast cells in the production of pig feed.

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Section: Effect Of Incubation Time On β-Glucanase Production Ability contrasting

confidence: 77%

Selection of Bacillus subtilis strains capable of producing β-glucanase supporting the hydrolysis of yeast cell walls

Kien

Linh

Hạnh

et al. 2020

FSAB

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“…The bacteria were grown in selective media consisting of different ingredients (g/L) i.e. K 2 HPO 4 (0.065), KH 2 PO 4 117 (0.25), (NH4) 2 SO 4 (0.05), NaCl (0.25), MgSO 4 .7H 2 O (0.012), yeast extract (0.15), 1% Laminarian at 37°C, 130 rpm for 96 h [ 47 ]. The cell free supernatant was obtained as described in section 2.2.1 and used as crude enzyme extract.…”

Section: Methodsmentioning

confidence: 99%

Bacillus spp., a bio-control agent enhances the activity of antioxidant defense enzymes in rice against Pyricularia oryzae

et al. 2017

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Plant growth promoting rhizobacteria (PGPR) are found to control the plant diseases by adopting various mechanisms. Induced systemic resistance (ISR) is an important defensive strategy manifested by plants against numerous pathogens especially infecting at aerial parts. Rhizobacteria elicit ISR by inducing different pathways in plants through production of various metabolites. In the present study, potential of Bacillus spp. KFP-5, KFP-7, KFP-17 was assessed to induce antioxidant enzymes against Pyricularia oryzae infection in rice. The antagonistic Bacillus spp. significantly induced antioxidant defense enzymes i-e superoxide dismutase (1.7–1.9-fold), peroxidase (3.5–4.1-fold), polyphenol oxidase (3.0–3.8-fold), phenylalanine ammonia-lyase (3.9–4.4-fold), in rice leaves and roots under hydroponic and soil conditions respectively. Furthermore, the antagonistic Bacillus spp significantly colonized the rice plants (2.0E+00–9.1E+08) and secreted multiple biocontrol determinants like protease (1.1–5.5 U/mg of soil or U/mL of hydroponic solution), glucanase, (1.0–1.3 U/mg of soil or U/mL of hydroponic solution), siderophores (6.5–42.8 μg/mL or mg) in the rhizosphere of different rice varieties. The results showed that treatment with Bacillus spp. enhanced the antioxidant defense activities in infected rice, thus alleviating P. oryzae induced oxidative damage and suppressing blast disease incidence.

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“…The main antagonistic mechanisms of the bacteria and yeasts in vitro against the phytopathogens are the production of hydrolytic enzymes, such as β-1,3-glucanase [38,39], which break the links of the glucan present in the cell wall of the phytopathogen, limiting their germination and growth. This enzyme has already been reported for B. subtilis [40], S. rhizophila [30,41,42], and C. laurentii [43]. The competence for space and nutrients is another mechanism through which the bacteria and yeasts limit phytopathogens in vitro because these microorganisms have a short period of exponential growth that allows them to exhaust carbon sources in their culture medium, thereby minimizing zoospore germination and phytopathogen growth [44,45].…”

Section: Discussionmentioning

confidence: 70%

Efficiency of Marine Bacteria and Yeasts on the Biocontrol Activity of Pythium ultimum in Ancho-Type Pepper Seedlings

et al. 2020

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Ancho-type pepper (Capsicum annuum L.) is a crop susceptible to Pythium ultimum, which has already been controlled with synthetic fungicide applications; however, marine antagonist microorganisms could be an alternative source of control. The efficiency in vitro and in vivo of marine bacteria and yeasts was determined against P. ultimum. The inhibition of the radial growth of P. ultimum was quantified in vitro by the bacteria Stenotrophomonas rhizophila KM01 and KM02; Bacillus subtilis RBM01 and RBM02, B. amyloliquefaciens 2RLBF and 3R4CF; and Pseudomonas spp. 2R6BF and 2RE9CF, as well as the yeasts Debaryomyces hansenii 1R11AB, 1R11CB, and LL01 and Cryptococcus laurentii 2R3BF and 2R1CB. The β-1,3-glucanase activity of the marine microorganisms was quantified in the presence of the phytopathogen. The disease index (DI), growth parameters, and colony forming units (CFU) were determined in ancho-type pepper plants inoculated with marine bacteria, yeasts, and P. ultimum. The radial zone of the phytopathogen was inhibited by 80% and 75% by S. rhizophila KM01 and C. laurentii 2R1CB, respectively. D. hansenii LL01 and S. rhizophila KM02 showed the highest activity of β-1,3-glucanase, with 6060 U/mL and 47 U/mL, respectively. B. subtilis RBM02 protected 100% of the plants from the oomycete, and an increase was quantified in all the growth parameters and CFU. The use of these marine bacteria and yeasts are, therefore, an option for P. ultimum biocontrol in ancho-type pepper plants, thereby minimizing the application of synthetic fungicides.Agronomy 2020, 10, 408 2 of 12 by immediate infection makes management of this phytopathogen very difficult [7]. Several Pythium species, including P. aphanidermatum, P. irregular, and P. ultimum, are known to cause damping-off and root rot diseases in pepper [8][9][10]. Although synthetic fungicides have shown promising results in controlling damping-off disease, phytotoxicity and fungicide residues pose serious problems to human and animal health, as well as the environment. In this context, phytosanitary measures and the management of pepper cultivation should include the application of microorganisms as biocontrol agents to minimize the use of synthetic fungicides [11].Bacteria and yeasts have a high capacity to control phytopathogens without causing damage to human and animal health and the environment [12,13]. Several mechanisms have been proposed for the biocontrol of phytopathogens by bacteria and yeasts, including competition for space and nutrients [14,15], lytic enzyme production such as β-1,3-glucanase [16,17], and induction of host resistance [18,19], among others. Both microorganisms have been isolated in different terrestrial ecosystems, mainly in plants, fruits, and soils; the main bacteria used as biocontrol agents are species of Stenotrophomonas, Bacillus, and Pseudomonas, which have already been reported for the control of Fusarium proliferatum in melon [20]; Sclerotium rolfsii [21] and Rhizoctonia solani in wheat [22]; and F. solani in cassava [23], among others. O...

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Medium optimization of beta-glucanase production by Bacillus subtilis SAHA 32.6 used as biological control of oil palm pathogen

Cited by 28 publications

References 29 publications

Selection of Bacillus subtilis strains capable of producing β-glucanase supporting the hydrolysis of yeast cell walls

Selection of Bacillus subtilis strains capable of producing β-glucanase supporting the hydrolysis of yeast cell walls

Bacillus spp., a bio-control agent enhances the activity of antioxidant defense enzymes in rice against Pyricularia oryzae

Efficiency of Marine Bacteria and Yeasts on the Biocontrol Activity of Pythium ultimum in Ancho-Type Pepper Seedlings

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