Medium optimization for the feather-degradation by Streptomyces fradiae Var S-221 using the response surface methodology

Cheng, Xin; Huang, Lin; Tu, Xiao-Rong; Li, Kun-tai

doi:10.1007/s10532-009-9286-7

Cited by 9 publications

(6 citation statements)

References 13 publications

(11 reference statements)

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“…The use of multivariate experimental design techniques, such as the Plackett-Burman and the Box-Behnken design, is becoming increasingly widespread in the optimization of many microbial cultures (Anbu et al 2007;Casarin et al 2008;Cai and Zheng 2009;Cheng et al 2010). However, the optimization of medium compositions and culture conditions for obtaining keratinase from thermophilic fungi has been little reported.…”

Section: Discussionmentioning

confidence: 99%

Optimal culture conditions for keratinase production by a novel thermophilicMyceliophthora thermophilastrain GZUIFR-H49-1

Liang

Han

Zhang

et al. 2011

Journal of Applied Microbiology

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Aims: To investigate the effect of medium compositions and culture conditions on keratinase production by a novel thermophilic fungus Myceliophthora thermophila (Apinis) Oorschot strain GZUIFR‐H49‐1. Methods and Results: The thermophilic strain GZUIFR‐H49‐1 with keratinolytic ability was characterized and identified as a strain of M. thermophila on the basis of its morphological characters and molecular analysis of ITS1‐5.8S‐ITS2 rDNA sequence. Among the medium compositions tested, the soluble starch (SS), urea, sodium thiosulfate and CaCl2 were the most effective C‐source, N‐source, S‐source and mineral ion, respectively, by employing the single‐factor experiment. The urea and pH value were the significant factors (P < 0·05) for the keratinase production in this experiment condition using Plackett–Burman factorial design. The conditions of keratinase production were further optimized by Box–Behnken design. Consequently, there was a 6·4‐fold increase (5100 U l−1) in the keratinase activity than the initial value (800 U l−1) by this optimal process. Conclusions: This study indicated that the optimization design proved a useful and powerful tool for the development of optimal medium compositions and culture conditions. Myceliophthora thermophila strain GZUIFR‐H49‐1 was a promising fungus strain for keratinase production. Significance and Impact of the Study: This study characterized a novel thermophilic M. thermophila strain GZUIFR‐H49‐1 with potential applications for keratinase production. These conditions of keratinase production obtained by means of optimization design will be accumulated as potential information for exploration and utilization to the new fungus isolate.

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Section: Discussionmentioning

confidence: 99%

Optimal culture conditions for keratinase production by a novel thermophilicMyceliophthora thermophilastrain GZUIFR-H49-1

Liang

Han

Zhang

et al. 2011

Journal of Applied Microbiology

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“…The absorbance was determined spectrophotometrically at 590 nm using UV-Vis spectrophotometer (UV-1800, Shimadzu). Total amino acid content was measured by manual ninhydrin method using leucine as standard (Cheng et al 2010). …”

Section: Time Course Study Of Feather Degradation In Basal Feather Mementioning

confidence: 99%

Ameliorating effects of chicken feathers in plant growth promotion activity by a keratinolytic strain of Bacillus subtilis PF1

Bhange

Chaturvedi

Bhatt

2016

Bioresour. Bioprocess.

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Background: Feathers are the major byproducts of poultry industry and considered as waste. Feathers (composed of protein keratin) are metabolized by a number of microorganisms as a source of carbon and nitrogen. Degradation of feathers results in production of amino acids and peptides, which can be employed as precursors for plant growthpromoting metabolites such as indole acetic acid, ammonia and HCN. The aim of the present investigation was to assess the influence of these metabolites (termed as feather protein hydrolysate) on plant growth promotion activity of keratinolytic bacterial strain Bacillus subtilis PF1.Results: Strain PF1 exhibits potent keratinolytic activity and can efficiently degrade 10 g/l chicken feathers under submerged cultivation with 81.4 ± 4.40 U/ml keratinase activity. Different concentrations of feathers supported the production of indole acetic acid by strain PF1. Strain PF1 produces maximum indole acetic acid (46.2 ± 0.21 µg/ml) in the presence of 2.0 % feathers at 120 h of incubation. The indole acetic acid production was confirmed by thin-layer chromatography and Fourier transform infrared spectroscopic analysis. However, increased concentration of feathers exhibited negative effect on phosphate solubilization due to increased alkalinity. HCN production also exhibited positive correlation with concentration of feathers. Finally, plant growth of Vigna radiata in the presence of strain PF1 with chicken feathers in soil was investigated, which showed good plant growth promotion activity. Increased ratio of C/N in soil also supported the plant growth promotion activity of feathers. Conclusion:Feather degradation property of B. subtilis PF1 could be efficiently utilized for feather waste management. The metabolites released by feather degradation along with strain PF1 could be successfully employed as an economic source of nitrogen fertilizers for plants.

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“…The absorbance was determined spectrophotometrically at 590 nm using UV–Vis spectrophotometer (UV-1800, Shimadzu). Total amino acid content was measured by manual ninhydrin method by taking leucine as standard (Cheng et al 2010 ).…”

Section: Methodsmentioning

confidence: 99%

Feather degradation potential of Stenotrophomonas maltophilia KB13 and feather protein hydrolysate (FPH) mediated reduction of hexavalent chromium

2016

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An efficient keratinolytic strain of Stenorophomonas maltophilia KB13 was isolated from feather disposal site of Bilaspur, Chhattisgarh, India. The strain could metabolize 10 g/l chicken feathers as sole source of carbon and nitrogen. Soluble protein, amino acid, and cysteine content were found to be maximum (690.6 ± 8.7, 688.9 ± 9.12 and 21 ± 0.36 µg/ml, respectively) at late logarithmic phase of growth. Protease and keratinase activity reached its maximum level (103.26 ± 7.09 and 178.5 ± 9.10 U/ml) at the 4th day of incubation. The feather protein hydrolysate (FPH) obtained after degradation of chicken feathers was utilized to reduce hexavalent chromium. About 78.4 ± 2.4 and 63.6 ± 2.2 % reduction of 50 and 100 mg/l Cr(VI), respectively, was observed after 60 min of incubation with FPH. Further, there was no effect of autoclaved FPH on Cr(VI) reduction indicating that any bacterial enzyme was not involved in reduction process. Cr(VI) reduction was significantly inhibited by 10 mm Hg2+ ions indicating the role of sulfur-containing amino acids in reduction process. FTIR analysis confirmed that chromium reduction occurred due to oxidation of amino acids cysteine and cystine. This study shows that FPH arising after feather degradation can be employed as a potential candidate for the reduction of hexavalant chromium.

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Medium optimization for the feather-degradation by Streptomyces fradiae Var S-221 using the response surface methodology

Cited by 9 publications

References 13 publications

Optimal culture conditions for keratinase production by a novel thermophilicMyceliophthora thermophilastrain GZUIFR-H49-1

Optimal culture conditions for keratinase production by a novel thermophilicMyceliophthora thermophilastrain GZUIFR-H49-1

Ameliorating effects of chicken feathers in plant growth promotion activity by a keratinolytic strain of Bacillus subtilis PF1

Feather degradation potential of Stenotrophomonas maltophilia KB13 and feather protein hydrolysate (FPH) mediated reduction of hexavalent chromium

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