Medium optimization for spore production ofConiothyrium minitans using statistically-based experimental designs

Ooijkaas, L.P.; Wilkinson, E.C.; Tramper, J.; Buitelaar, R.M.

doi:10.1002/(sici)1097-0290(19990705)64:1<92::aid-bit10>3.0.co;2-8

Cited by 74 publications

(15 citation statements)

References 21 publications

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“…Using a fraction of the factorial design without losing information about the main effects of variables can reduce the number of experiments. [ 15 ]…”

Section: Methodsmentioning

confidence: 99%

Optimization and purification of mannanase produced by an alkaliphilic-thermotolerantBacillus cereusN1 isolated from Bani Salama Lake in Wadi El-Natron

El-Sharouny

El-Toukhy

El‐Sersy

et al. 2015

Biotechnology & Biotechnological Equipment

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An alkaliphilic-thermotolerant Bacillus cereus N1 isolated from Bani Salama Lake, Wadi El-Natron, Egypt, was proved to produce mannanase enzyme. Optimization of the fermentation medium components using Plackett–Burman design was applied. Glucose and inoculum size were found to be the most significant factors enhancing the production of the enzyme. On applying optimized medium in the fermentation process, an enzyme productivity of 42.2 UmL−1 was achieved with 6.4 fold increase compared to the basal one. Mannanase was also extracted and purified using chromatography such as ion-exchange chromatographic and gel filtration methods. It was indicated that, the mannanase activity extracted and purified from the isolate B. cereus N1 was reduced to 321.6 U (about 36% of the whole mannanase in the culture filtrate) in comparison with the initial mannanase activity (900 U) and the total protein content reduced to 52 mg (the initial total protein content was 220 mg). However, the specific activity for the mannanase from B. cereus N1 at the end of the purification steps was found to be about 628 Umg−1 compared to 4.2 Umg−1 at the initial culture filtrate. It was also indicated that the mannanase enzyme was purified almost 149-fold.

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“…Using a fraction of the factorial design without losing information about the main effects of variables can reduce the number of experiments. [ 15 ]…”

Section: Methodsmentioning

confidence: 99%

Optimization and purification of mannanase produced by an alkaliphilic-thermotolerantBacillus cereusN1 isolated from Bani Salama Lake in Wadi El-Natron

El-Sharouny

El-Toukhy

El‐Sersy

et al. 2015

Biotechnology & Biotechnological Equipment

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“…In the conventional method, the medium is optimized by changing one factor at a time while keeping others at a constant level (26,33), which is laborious and often leads to wrong conclusions (20). Multivariate experiments are designed not only to reduce the number of necessary experiments in the optimization process, but also to produce more defined results than those available by univariate strategies (27). Plackett-Burman (P-B) and response-surface methodology (RSM) are multivariate analyzing tools comprising of mathematical and statistical techniques for generating empirical models that obtained importance for optimizing production conditions of industrially high value products such as chemicals, enzymes, and for studying enzyme kinetics.…”

Section: Introductionmentioning

confidence: 99%

Optimization of nutrition factors on chitinase production from a newly isolated Chitiolyticbacter meiyuanensis SYBC-H1

Hao¹,

Cai²,

Liao³

et al. 2012

Braz. J. Microbiol.

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The present study reports statistical medial optimization for chitinase production by a novel bacterial strain isolated from soil recently, which the name Chitinolyticbacter meiyuanensis SYBC-H1 is proposed. A sequential statistical methodology comprising of Plackett-Burman and response surface methodology (RSM) was applied to enhance the fermentative production of chitinase, in which inulin was firstly used as an effective carbon source. As a result, maximum chitinase activity of 5.17 U/mL was obtained in the optimized medium, which was 15.5-fold higher than that in the basal medium. The triplicate verification experiments were performed under the optimized nutrients levels which indicated that it well agreed with the predicted value

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“…In addition, the Plackett-Burman design applied in the present study for the optimization of production of the enzyme by free cells has been demonstrated as an efficient approach to screen for medium component and/or factors affecting the production of cellulase, where medium optimization is generally a timeconsuming and labor-intensive process. Statistically based experimental designs proved to be valuable tools in optimization culture conditions (Zhang et al, 1996;Ooijkass et al, 1998). One of the advantages of applying multi-factorial experiments is that it considers the interaction between the non-linear natures of the response in short experiments (Gresham and Inamin, 1986).…”

Section: Discussionmentioning

confidence: 99%

Cellulases Production by Free and Immobilized Aspergillus flavus

2014

Egypt. J. Bot.

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HE PRODUCTION of cellulase from some local fungal isolates ……..was studied. The results showed that Aspergillus flavus produced the highest cellulase activity (3.017 U/ml) under static condition. It also showed that the best carbon source for cellulase production was filter papers (7.0932 U/ml), and onion scales (6.396 U/ml) at a concentration 30 g/l medium. The Plackett-Burman design was studied for cellulase production from A. flavus showed that the optimizing medium achieved by the statistical design increased cellulase activity (11.699 U/ml) which approximately 2.3 times than that obtained from the basal medium. In addition, A. flavus biomass was entrapped in different gel materials, and the best one for producing cellulase was agar (8.22 U/ml), however, this activity was lower than that in free cultures. Moreover, A. flavus biomass was adsorbed on different porous materials, and the cultures adsorbed on luffa pulp exerted the highest cellulase activity (17.78 U/ml) which was higher than that produced by free cultures. By reusing the adsorbed A. flavus biomass on luffa pulp in seven successive times, the 4 th cycle gave the highest cellulase activity (34.17 U/ml). It was concluded that A. flavus is a feasible candidate for the production of cellulase activity which can be applied in many industrial fields. The results also indicate the possibility of using different agro-wastes like onion scales as a sole carbon source for production of cellulase by A. flavus.

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Medium optimization for spore production ofConiothyrium minitans using statistically-based experimental designs

Cited by 74 publications

References 21 publications

Optimization and purification of mannanase produced by an alkaliphilic-thermotolerantBacillus cereusN1 isolated from Bani Salama Lake in Wadi El-Natron

Optimization and purification of mannanase produced by an alkaliphilic-thermotolerantBacillus cereusN1 isolated from Bani Salama Lake in Wadi El-Natron

Optimization of nutrition factors on chitinase production from a newly isolated Chitiolyticbacter meiyuanensis SYBC-H1

Cellulases Production by Free and Immobilized Aspergillus flavus

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