Medium optimization for a novel crude-oil degrading lipase from Pseudomonas aeruginosa SL-72 using statistical approaches for bioremediation of crude-oil

Verma, Shikha; Saxena, Jyoti; Prasanna, Radha; Sharma, Vinay; Nain, Lata

doi:10.1016/j.bcab.2012.07.002

Cited by 33 publications

(15 citation statements)

References 25 publications

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“…These results suggested that the P. aeruginosa HFE733 fermentation broth was effective for oil removal and COD decrease, and can be used for food wastewater treatment. Similar studies have been carried out with other lipase-producing strains, such as P. aeruginosa SL-72 [33] and Aspergillus awamori BTMFW032 [34]. The crude lipase of P. aeruginosa SL-72 was added to wastewater contaminated with crude oil, resulting in degradation of 82.83% of the oil content and 86.39% reduction of COD after 7 days of treatment, slightly lower than the effect of the HFE733 fermentation broth.…”

Section: Biodegradation In Food Wastewater Treatmentmentioning

confidence: 71%

Purification and characterization of alkaline lipase production byPseudomonas aeruginosaHFE733 and application for biodegradation in food wastewater treatment

Cai

Wang

et al. 2018

Biotechnology & Biotechnological Equipment

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An alkaline lipase strain of Pseudomonas aeruginosa HFE733 was isolated from soil samples of domestic waste. The alkaline lipase from P. aeruginosa HFE733 was purified and characterized. The enzyme was purified 9.97-fold by means of ammonium sulphate precipitation, Sephadex G-25 and diethylaminoethyl (DEAE) cellulose chromatography. Purified alkaline lipase protein was analyzed by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE), the molecular mass was 51.0 kDa. The enzyme exhibited maximum activity at 40 C and pH 8.5. The enzyme is stable at pH 7.0-8.5. It was remarkably activated by some metal ions and chemical reagents, such as Fe 3+ , Al 3+ , b-mercaptoethanol, cysteine, DL-dithiothreitol (DTT), Tween 80 and Triton X-100, but suppressed by sodium dodecyl sulfate (SDS) at 10 mmol/L. The P. aeruginosa HFE733 strain and lipase exhibited remarkable potential for biodegradation of oil and organics (measured as chemical oxygen demand (COD)). We demonstrated that it can be used for biodegradation of food wastewater from restaurants.

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Section: Biodegradation In Food Wastewater Treatmentmentioning

confidence: 71%

Purification and characterization of alkaline lipase production byPseudomonas aeruginosaHFE733 and application for biodegradation in food wastewater treatment

Cai

Wang

et al. 2018

Biotechnology & Biotechnological Equipment

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“…Statistical methods have been adopted to optimize the lipase production process as well as the process of oil hydrolysis by Pseudomonas sp. (Gaur and Khare 2011;Verma et al 2012). B. stearothermophilus isolated from slaughter house waste and a bacterial pool of Bacillus spp.…”

Section: Potential Microbial Strains For Oandg Bioremediationmentioning

confidence: 99%

An insight into microbial lipases and their environmental facet

Kanmani¹,

Aravind²,

Kumaresan³

2014

Int. J. Environ. Sci. Technol.

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Lipases are serine hydrolases that catalyze the hydrolysis and synthesis of esters formed from glycerol and long-chain fatty acids, by acting at the oil-water interface. Lipases from microbial sources have received heightened attention for an array of industrial applications, and these enzymes have been well exploited in the environmental sector as well. In this article, we present an overview of microbial lipase, including the microorganisms from which it could be produced; the application of recombinant DNA technology tools to produce lipase with enhanced properties, the effective use of waste materials as substrates for lipase production; the usage of statistical tools to efficiently optimize the production medium; lipase purification strategies; and the immobilization of the enzyme on a variety of support materials. The next section of the article provides a gist of its application in diversified spheres and focusses exclusively on the environmentally relevant ones. Lipasecatalyzed esterification, transesterification, and interesterification reactions, an emerging area of green chemistry; lipase-mediated in vitro biopolymer synthesis and degradation; and the application of lipase for remediating fat and oil constituents in wastewater are dealt with in-depth. When its full potential is harnessed, the enzyme could play a pivotal role in environmental management.

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“…Bioremediation is the process employed to decontaminate samples from oil spills, oil‐wet soils, industrial wastes, and wastewater tinged with lipids that can otherwise have hazardous consequences when they enter natural resources without prior treatment. Lipase‐catalyzed bioremediation steps can be adopted to treat the wastes effectively from lipid‐processing factories and restaurants . Lipase‐producing organisms such as Staphylococcus pasteuri COM‐4A, B. subtilis COM‐B6, Arthrobacter sps.…”

Section: Applications Of Lipase‐catalyzed Reactionsmentioning

confidence: 99%

“…Lipase-catalyzed bioremediation steps can be adopted to treat the wastes effectively from lipidprocessing factories and restaurants. 281 Lipase-producing organisms such as Staphylococcus pasteuri COM-4A, B. subtilis COM-B6, Arthrobacter sps. etc.…”

Section: Bioremediationmentioning

confidence: 99%

Recent advances on sources and industrial applications of lipases

Sarmah

Revathi

Sheelu

et al. 2017

Biotechnology Progress

297

148

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Lipases are the industrially important biocatalysts, which are envisioned to have tremendous applications in the manufacture of a wide range of products. Their unique properties such as better stability, selectivity and substrate specificity position them as the most expansively used industrial enzymes. The research on production and applications of lipases is ever growing and there exists a need to have a latest review on the research findings of lipases. The present review aims at giving the latest and broadest overall picture of research and development on lipases by including the current studies and progressions not only in the diverse industrial application fields of lipases, but also with regard to its structure, classification and sources. Also, a special emphasis has been made on the aspects such as process optimization, modeling, and design that are very critical for further scale-up and industrial implementation. The detailed tabulations provided in each section, which are prepared by the exhaustive review of current literature covering the various aspects of lipase including its production and applications along with example case studies, will serve as the comprehensive source of current advancements in lipase research. This review will be very useful for the researchers from both industry as well as academia in promoting lipolysis as the most promising approaches to intensified, greener and sustainable processes. © 2017 American Institute of Chemical Engineers Biotechnol. Prog., 34:5-28, 2018.

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Medium optimization for a novel crude-oil degrading lipase from Pseudomonas aeruginosa SL-72 using statistical approaches for bioremediation of crude-oil

Cited by 33 publications

References 25 publications

Purification and characterization of alkaline lipase production byPseudomonas aeruginosaHFE733 and application for biodegradation in food wastewater treatment

Purification and characterization of alkaline lipase production byPseudomonas aeruginosaHFE733 and application for biodegradation in food wastewater treatment

An insight into microbial lipases and their environmental facet

Recent advances on sources and industrial applications of lipases

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