Medium-Chain fatty acids and long-chain alcohols production from waste activated sludge via two-stage anaerobic fermentation

Wu, Shu-Lin; Wei, Wei; Sun, Jing; Xu, Qiuxiang; Dai, Xiaohu; Ni, Bing‐Jie

doi:10.1016/j.watres.2020.116381

Cited by 98 publications

(39 citation statements)

References 54 publications

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“…The abundance of Oscillibacter was significantly increased in HFD-fed mice without B. adolescentis treatment, which was consistent with the findings of previous studies [16,35]. Oscillibacter is able to produce SCFAs and medium-chain fatty acids [36,37], which provide additional energy sources for the HFD-fed mice. Bacteroides is a dominant genus in the human intestine and plays key roles in maintaining the stability of the gut microbiota, host immunity, and energy metabolism, possessing enormous potential to be a next-generation probiotic [38][39][40].…”

Section: Discussionsupporting

confidence: 91%

Bifidobacterium adolescentis Isolated from Different Hosts Modifies the Intestinal Microbiota and Displays Differential Metabolic and Immunomodulatory Properties in Mice Fed a High-Fat Diet

et al. 2021

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The incidence of obesity, which is closely associated with the gut microbiota and chronic inflammation, has rapidly increased in the past 40 years. Therefore, the probiotic-based modification of the intestinal microbiota composition has been developed as a strategy for the treatment of obesity. In this study, we selected four Bifidobacterium adolescentis strains isolated from the feces of newborn and elderly humans to investigate whether supplementation with B. adolescentis of various origins could alleviate obesity in mice. Male C57BL/6J mice fed a high-fat diet (HFD, 60% energy as fat) received one of the following 14-week interventions: (i) B. adolescentis N4_N3, (ii) B. adolescentis Z25, (iii) B. adolescentis 17_3, (iv) B. adolescentis 2016_7_2, and (v) phosphate-buffered saline. The metabolic parameters, thermogenesis, and immunity of all treated mice were measured. Cecal and colonic microbial profiles were determined by 16S rRNA gene sequencing. Intestinal concentrations of short-chain fatty acids (SCFAs) were measured by gas chromatography-mass spectrometry (GC-MS). The B. adolescentis strains isolated from the feces of elderly humans (B. adolescentis Z25, 17_3, and 2016_7_2) decreased the body weight or weight gain of mice, whilst the strain isolated from the newborn (B. adolescentis N4_N3) increased the body weight of mice. The B. adolescentis strains isolated from the elderly also increased serum leptin concentrations and induced the expression of thermogenesis- and lipid metabolism-related genes in brown adipose tissue. All the B. adolescentis strains alleviated inflammations in the spleen and brain and modified the cecal and colonic microbiota. Particularly, all strains reversed the HFD-induced depletion of Bifidobacterium and reduced the development of beta-lactam resistance. In addition, the B. adolescentis strains isolated from the elderly increased the relative abundances of potentially beneficial genera, such as Bacteroides, Parabacteroides, and Faecalibaculum. We speculate that such increased abundance of commensal bacteria may have mediated the alleviation of obesity, as B. adolescentis supplementation decreased the intestinal production of SCFAs, thereby reducing energy delivery to the host mice. Our results revealed that certain strains of B. adolescentis can alleviate obesity and modify the gut microbiota of mice. The tested strains of B. adolescentis showed different effects on lipid metabolism and immunity regulation, with these effects related to whether they had been isolated from the feces of newborn or elderly humans. This indicates that B. adolescentis from different sources may have disparate effects on host health possibly due to the transmission of origin-specific functions to the host.

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Section: Discussionsupporting

confidence: 91%

Bifidobacterium adolescentis Isolated from Different Hosts Modifies the Intestinal Microbiota and Displays Differential Metabolic and Immunomodulatory Properties in Mice Fed a High-Fat Diet

et al. 2021

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“…In the CE process, in addition to SCFAs, the essential metabolic condition for the production of MCFAs also requires the participation of an electron donor (ED), which could produce the key intermediate acetyl-CoA as the cyclic molecule elongating the carbon chain length of SCFAs. , Ethanol was found to be a highly suitable ED for performing CE, as it could result in a relatively high MCFA synthesis rate . Also, ethanol is regarded as the most favorable substrate for n -caproate synthesis by Clostridium kluyveri , which is a key microorganism involved in the CE process .…”

Section: Introductionmentioning

confidence: 99%

“…Also, ethanol is regarded as the most favorable substrate for n -caproate synthesis by Clostridium kluyveri , which is a key microorganism involved in the CE process . Nevertheless, at higher carbon ratios of ethanol to SCFAs (≥3:1), a large amount of SCFAs remained without further participation in CE process . Meanwhile, when the concentration of produced MCFAs is too high and beyond the dissociation equilibrium, the undissociated MCFAs have greater antimicrobial properties for restraining the further production of MCFAs …”

Section: Introductionmentioning

confidence: 99%

“…6,8 Ethanol was found to be a highly suitable ED for performing CE, as it could result in a relatively high MCFA synthesis rate. 9 Also, ethanol is regarded as the most favorable substrate for ncaproate synthesis by Clostridium kluyveri, which is a key microorganism involved in the CE process. 10 Nevertheless, at higher carbon ratios of ethanol to SCFAs (≥3:1), a large amount of SCFAs remained without further participation in CE process.…”

Section: ■ Introductionmentioning

confidence: 99%

“…10 Nevertheless, at higher carbon ratios of ethanol to SCFAs (≥3:1), a large amount of SCFAs remained without further participation in CE process. 9 Meanwhile, when the concentration of produced MCFAs is too high and beyond the dissociation equilibrium, the undissociated MCFAs have greater antimicrobial properties for restraining the further production of MCFAs. 11 Biochar derived from pine was recently proposed as a beneficial material that could promote the utilization of SCFAs and increase the level of production of MCFAs.…”

Section: ■ Introductionmentioning

confidence: 99%

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Revealing the Mechanism of Biochar Enhancing the Production of Medium Chain Fatty Acids from Waste Activated Sludge Alkaline Fermentation Liquor

Wei

et al. 2021

ACS EST Water

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In this work, the effectiveness of biochar with distinctively different particle sizes (i.e., 2000−5000 and 75−150 μm) derived from anaerobically digested sludge was comprehensively evaluated in the production of enhancing MCFAs (medium chain fatty acids) using waste activated sludge alkaline fermentation liquor as feedstock with ethanol as the electron donor. The results showed that fine biochar (i.e., 75−150 μm) was more favorable for enhancing the production of MCFAs with an increased electron transfer efficiency reaching 92.6%. Metagenomic analysis revealed that the total microbial taxonomic composition shifted in favor of the production of MCFAs (e.g., Clostridium kluyveri sp., Clostridium magnum sp., and Clostridium botulinum sp.). The increased relative abundance of functional enzymes associated with the production of MCFAs confirmed that both the reverse β-oxidation pathway and the fatty acid biosynthesis pathway were enhanced by biochar. Mechanistic studies revealed that the biochar had high redoxactive properties due to the functional groups (mainly quinones and hydroquinone) on its surface and an important ability to charge and/or discharge, which thus promoted the transfer of electrons for the production of MCFAs during the CE process.

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Dose-response and type-dependent effects of antiviral drugs in anaerobic digestion of waste-activated sludge for biogas production

Wang,

Yang,

Cai

et al. 2023

Environ Sci Pollut Res

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In the context of the COVID-19 pandemic, antiviral drugs (AVDs) were heavily excreted into wastewater and subsequently enriched in sewage sludge due to their widespread use. The potential ecological risks of AVDs have attracted increasing attention, but information on the effects of AVDs on sludge anaerobic digestion (AD) is limited. In this study, two typical AVDs (lamivudine and ritonavir) were selected to investigate the responses of AD to AVDs by biochemical methane potential tests. The results indicated that the effects of AVDs on methane production from sludge AD were dose- and type-dependent. The increased ritonavir concentration (0.05–50 mg/kg TS) contributed to an 11.27–49.43% increase in methane production compared with the control. However, methane production was significantly decreased at high lamivudine doses (50 mg/kg TS). Correspondingly, bacteria related to acidification were affected when exposed to lamivudine and ritonavir. Acetoclastic and hydrotropic methanogens were inhibited at a high lamivudine dose, while ritonavir enriched methylotrophic and hydrotropic methanogens. Based on the analysis of intermediate metabolites, the inhibition of lamivudine and the promotion of ritonavir on acidification and methanation were confirmed. In addition, the existence of AVDs could affect sludge properties. Sludge solubilization was inhibited when exposed to lamivudine and enhanced by ritonavir, perhaps caused by their different structures and physicochemical properties. Moreover, lamivudine and ritonavir could be partially degraded by AD, but 50.2–68.8% of AVDs remained in digested sludge, implying environmental risks. Supplementary Information The online version contains supplementary material available at 10.1007/s11356-023-27045-7.

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Medium-Chain fatty acids and long-chain alcohols production from waste activated sludge via two-stage anaerobic fermentation

Cited by 98 publications

References 54 publications

Bifidobacterium adolescentis Isolated from Different Hosts Modifies the Intestinal Microbiota and Displays Differential Metabolic and Immunomodulatory Properties in Mice Fed a High-Fat Diet

Bifidobacterium adolescentis Isolated from Different Hosts Modifies the Intestinal Microbiota and Displays Differential Metabolic and Immunomodulatory Properties in Mice Fed a High-Fat Diet

Revealing the Mechanism of Biochar Enhancing the Production of Medium Chain Fatty Acids from Waste Activated Sludge Alkaline Fermentation Liquor

Dose-response and type-dependent effects of antiviral drugs in anaerobic digestion of waste-activated sludge for biogas production

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