Mediterranean Fruit Fly
1
: Dispersal of Wild and Irradiated and Untreated Laboratory-Reared Males
2

Wong, Tim T. Y.; Whitehand, Linda C.; Kobayashi, Richard M.; Ohinata, K.; Tanaka, Norimitsu; Harris, E. J.

doi:10.1093/ee/11.2.339

Cited by 58 publications

(48 citation statements)

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“…Alguns trabalhos de marcação e recaptura demonstraram que machos estéreis podem sobreviver, em alguns casos, por muitos dias após a sua liberação em campo (Wong et al 1982, Plant & Cunningham 1991. Porém, como os resultados do presente trabalho indicam que a senescência tem efeito negativo no sucesso de cópula dos machos criados em laboratório, a questão se os machos estéreis são ou não mais longevos assume menor relevância para a técnica do macho estéril do que seu sucesso de cópula nos primeiros dias após a liberação.…”

Section: Discussionunclassified

“…Trabalhos de marcação e recaptura demonstraram que machos estéreis de C. capitata podem sobreviver por vários dias após liberação (Wong et al 1982, Plant & Cunningham 1991. Assim, estudos que abordem o papel da senescência no sucesso de cópula dos mesmos são relevantes para determinar o período de atividade sexual após a liberação dos machos e também para verifi car se a maior sobrevivência no campo refl ete em sucesso reprodutivo.…”

Section: Palavras-chave: Mosca-das-frutas Comportamento Sexualunclassified

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Escolha de parceiro para acasalamento em Ceratitis capitata (Wiedemann) (Diptera: Tephritidae): influência do envelhecimento dos machos no sucesso de cópula

Neto¹,

Dias²,

Joachim‐Bravo³

2009

Neotrop. entomol.

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-The objective of this study was to evaluate the effect of male ageing on male pheromone release and mating success of Ceratitis capitata (Wiedemann). The effects of male ageing on mating were evaluated on fi ve and 21 d-old males by assessing their mating success (males chosen by a female for copulation) and the amount of males releasing the sex pheromone. The mating success was evaluated by using several ratios of young to older males by increasing the number of older males:young males from 1:1 to 5:1. The mating success of the 1:1 ratio was also evaluated in fi eld cages. The evaluation of the mating success (in the 1:1 ratio) showed a clear preference of the females for young males. Sex pheromone emission was much more common on young than older males. Even in cases were older males were more abundant (ratios 2:1 and 3:1), females still chose the young males. However, females could not distinguish young from older males in ratios of 4:1 or 5:1. Our data indicate that the ageing of C. capitata males has a considerable negative effect on their reproductive success, especially if they are found in a proportion any lower than 3:1.KEY WORDS: Fruit fl y, sex behavior RESUMO -O objetivo deste estudo foi avaliar o efeito da senescência no sucesso de cópula e na secreção de feromônio sexual por machos de Ceratitis capitata (Wiedemann). O efeito do envelhecimento do macho sobre o acasalamento foi analisado utilizando-se machos com cinco e 21 dias de idade, através do seu sucesso de copula (escolha dos machos pela fêmea para cópula) e pela quantidade de machos que emitiram feromônio sexual. O sucesso de cópula foi avaliado através de várias proporções de machos jovens em relação a machos velhos pelo aumento do número de machos velhos:machos jovens de 1:1 até 5:1. O sucesso de cópula na proporção de 1:1 também foi avaliado em gaiola de campo. A avaliação do sucesso de cópula (na proporção 1:1) demonstrou clara preferência das fêmeas pelos machos jovens. A emissão de feromônio sexual foi mais comum em machos jovens do que em machos velhos. Mesmo em casos onde os machos velhos foram mais abundantes (proporções 2:1 e 3:1), as fêmeas continuaram escolhendo os machos jovens. Entretanto, as fêmeas não distinguiram mais os machos jovens em relação aos machos velhos nas proporções 4:1 ou 5:1. Os resultados indicam que o envelhecimento dos machos de C. capitata tem considerável efeito negativo sobre seu sucesso reprodutivo, especialmente se eles são encontrados em proporção menor do que 3:1.

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Section: Discussionunclassified

Section: Palavras-chave: Mosca-das-frutas Comportamento Sexualunclassified

Escolha de parceiro para acasalamento em Ceratitis capitata (Wiedemann) (Diptera: Tephritidae): influência do envelhecimento dos machos no sucesso de cópula

Neto¹,

Dias²,

Joachim‐Bravo³

2009

Neotrop. entomol.

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“…The effect of the sterilizing irradiation process on mass-reared medflies has been thoroughly studied for quality control reasons with emphasis on parameters such as emergence, longevity, mating competitiveness, and dispersal (Holbrook and Fujimoto, 1970;Zumreoglu et al, 1979;Wong et al, 1982;Barry et al, 2003). However, the effect of irradiation on the gut microbiota has received little scrutiny.…”

Section: Introductionmentioning

confidence: 99%

Manipulation of the microbiota of mass-reared Mediterranean fruit flies Ceratitis capitata (Diptera: Tephritidae) improves sterile male sexual performance

2009

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The sterile insect technique (SIT) is a method of biological control whereby millions of factory reared sterile male insects are released into the field. This technique is commonly used to combat the Mediterranean fruit fly (Ceratitis capitata, Diptera: Tephritidae). Sterile medfly males are less competent in attracting and mating with wild females, a property commonly linked to the irradiation process responsible for the sterilization. As bacteria are important partners in the fly's life cycle, we used molecular analytical methods to study the community structure of the gut microbiota in irradiated male medflies. We find that the sterilizing irradiation procedure affects the gut bacterial community structure of the Mediterranean fruit fly. Although the Enterobacteriaceae family remains the dominant bacterial group present in the gut, the levels of Klebsiella species decreases significantly in the days after sterilization. In addition, we detected substantial differences in some bacterial species between the mass rearing strain Vienna 8 and the wild strain. Most notable among these are the increased levels of the potentially pathogenic species Pseudomonas in the industrial strain. Testing the hypothesis that regenerating the original microbiota community could result in enhanced competitiveness of the sterile flies, we found that the addition of the bacterial species Klebsiella oxytoca to the postirradiation diet enables colonization of these bacteria in the gut while resulting in decreased levels of the Pseudomonas sp. Feeding on diets containing bacteria significantly improved sterile male performance in copulatory tests. Further studies will determine the feasibility of bacterial amelioration in SIT operations.

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“…The production process, however, and in particular the need to sterilize the insects by irradiation of puparia, can negatively affect adult performance. The effect of irradiation on medflies has been thoroughly studied for quality control parameters, including emergence, longevity, mating competitiveness, and dispersal (Holbrook and Fujimoto, 1970;Zumreoglu et al, 1979;Wong et al, 1982;Barry et al, 2002Barry et al, , 2003. All these studies suggest that although sterile massreared medflies do join and compete within leks, and induce sterility in offspring, they are clearly at a disadvantage with wild males when competing for wild females (Shelly et al, 1994;Lance et al, 2000;Barry et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Effects of gamma‐irradiation on midgut proteolytic activity of the mediterranean fruit fly, Ceratitis capitata (Diptera: Tephritidae)

Andrés¹,

Ortego²,

Castañera³

2007

Arch Insect Biochem Physiol

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The Mediterranean fruit fly (medfly), Ceratitis capitata (Wiedemann), is a key pest of citrus in Spain because of significant yield losses and to quarantine restrictions. Biologically based control methods, such as the Sterile Insect Technique (SIT), which relies on the sterilization by irradiation of large numbers of insects, is gaining an increasing role in the control of medfly in Mediterranean areas. However, gamma-irradiation might damage the midgut epithelium cells, causing a lowering of nutritive assimilation that can negatively affect adult performance. Irradiation effects on digestive physiology are well established for a number of insect pests, but there is no information on medfly. Our aim was to determine the effects of gamma-irradiation on C. capitata digestive protease activity. Both larvae and adults were found to use a similar proteolytic system based on aspartyl-, trypsin-, chymotrypsin-, amino peptidase-, and carboxypeptidase A- and B-like activities. Pupae of the Vienna-7 (tsl) strain were irradiated at 70 or 140 Gy, two days before emergence, and the adults fed during 5 days on sugar-protein (4:1) diets. Protease activity was measured in midgut extracts and compared with males non-irradiated reared in the same conditions. The results showed that the irradiation doses tested had no effect on the digestive proteolytic activities of medfly adults. Moreover, the longevity of irradiated medflies at the highest dose (140 Gy) was similar to that of controls.

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Mediterranean Fruit Fly 1 : Dispersal of Wild and Irradiated and Untreated Laboratory-Reared Males 2

Cited by 58 publications

References 0 publications

Escolha de parceiro para acasalamento em Ceratitis capitata (Wiedemann) (Diptera: Tephritidae): influência do envelhecimento dos machos no sucesso de cópula

Escolha de parceiro para acasalamento em Ceratitis capitata (Wiedemann) (Diptera: Tephritidae): influência do envelhecimento dos machos no sucesso de cópula

Manipulation of the microbiota of mass-reared Mediterranean fruit flies Ceratitis capitata (Diptera: Tephritidae) improves sterile male sexual performance

Effects of gamma‐irradiation on midgut proteolytic activity of the mediterranean fruit fly, Ceratitis capitata (Diptera: Tephritidae)

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