Medicinal signaling cells niche in stromal vascular fraction from lipoaspirate and microfragmented counterpart

Zenić, Lucija; Polančec, Denis; Hudetz, Damir; Jeleč, Željko; Rod, Eduard; Vidović, Dinko; Silva, Mario; Sabalić, Srećko; Vrdoljak, Trpimir; Petrović, Tadija; Čukelj, Fabijan; Molnar, Vilim; Čemerin, Martin; Matišić, Vid; Brlek, Petar; Koroljević, Zrinka Djukić; Borić, Igor; Lauc, Gordan; Primorac, Dragan

doi:10.3325/cmj.2022.63.265

Cited by 4 publications

(4 citation statements)

References 44 publications

(43 reference statements)

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“…Autologous micro-fragmented adipose tissue contains mesenchymal stem cells (MSCs) that additionally have regenerative and immunomodulatory effects. Moreover, recent studies revealed gender-dependent differences in the stromal vascular fraction from lipoaspirate and micro-fragmented adipose tissue, which implies different therapeutic responses are needed in men and women [13]. These new findings indicate a potential beneficial effect of cell therapy in hair growth stimulation in addition to the currently tested PRP treatment.…”

Section: Discussionmentioning

confidence: 87%

Autologous Platelet-Rich Plasma (PRP) for Treating Androgenetic Alopecia: A Novel Treatment Protocol Standardized on 2 Cases

Maletic¹,

Dumić-Čule

Brlek

et al. 2022

JCM

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Platelet-rich plasma (PRP) treatment has emerged in recent years as a valuable, effective, and affordable treatment for androgenetic alopecia. Androgenetic alopecia is the most common type of alopecia, affecting both men and women, and is characterized by diminished hair follicles mainly pronounced in the frontal region and vertex. A considerable variety of PRP treatment regimens have been described so far, but there is no consensus on the standardization of PRP preparation or administration protocol. Our study was conducted on two patients to test the efficacy of a new PRP application protocol of only two treatments by using a combination of a PRP collecting device and a conventional kit. Efficacy of treatment was assessed after a 6-month follow-up by artificial intelligence (AI)-driven software on microscopic images of treated regions. An average number of hairs, cumulative hair thickness, and the number of follicular units increased in the vertex region of both patients by 30/59%, 35/53%, and 14/48%, respectively. The novel treatment regimen showed significant effectiveness in only six months.

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Section: Discussionmentioning

confidence: 87%

Autologous Platelet-Rich Plasma (PRP) for Treating Androgenetic Alopecia: A Novel Treatment Protocol Standardized on 2 Cases

Maletic¹,

Dumić-Čule

Brlek

et al. 2022

JCM

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“…Mesenchymal stem cells (MSCs) are multipotent adult stem cells that have the ability to differentiate into a variety of cell types (adipocytes, osteoblasts, myocytes, chondrocytes, etc.) dictated by the given milieu [ 35 , 40 , 41 , 42 , 47 , 48 , 49 ]. Regarding origin, MSCs can be extracted from the bone marrow (BM-MSCs), umbilical cord MSCs (UC-MSCs), amniotic fluid SCs (AF-SC), placental MSCs (P-MSC), menstrual blood SCs (Men-SC), breast milk, cervix, dental tissue, synovial tissue, and fluid, or can be adipose-derived MSCs (AD-MSCs) and amnion-derived MSCs.…”

Section: Discussionmentioning

confidence: 99%

“…Via polychromatic flow cytometric analysis, earlier, we determined the phenotypes of the CD45 subpopulation in autologous micro-fragmented samples containing the stromal vascular fraction (SVF). The most predominant cells include endothelial progenitors (EP) (CD31+ CD34+ CD73± CD90± CD105± CD146±), mature endothelial cells (CD31+ CD34− CD73± CD90± CD105− CD146±), pericytes (CD31− CD34− CD73± CD90+ CD105− CD146+), transitional pericytes (CD31− CD34+ CD73± CD90+ CD105− CD146+), and supra-adventitial-adipose stromal cells (CD31− CD34+ CD73high CD90+ CD105− CD146−) [ 48 , 49 ]. On the other hand, MSCs express very low levels of surface antigens and therefore do not trigger an immune response, allowing their usage as therapeutic agents in regenerative medicine [ 50 , 51 , 52 , 53 ].…”

Section: Discussionmentioning

confidence: 99%

Novel Approach in Rectovaginal Fistula Treatment: Combination of Modified Martius Flap and Autologous Micro-Fragmented Adipose Tissue

Dimova,

Erceg Ivkošić,

Brlek

et al. 2023

Biomedicines

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In this paper, we introduce an innovative therapeutic approach for managing rectovaginal fistulas (RVF), by combining the modified Martius flap and micro-fragmented adipose tissue (MFAT) enriched with mesenchymal stem cells (MSC). This novel approach aims to deal with the difficulties associated with RVF, a medically complex condition with a lack of effective treatment options. We present the case of a 45-year-old female patient with a 15-year history of Crohn’s disease (CD). During the preceding eight years, she had encountered substantial difficulties resulting from a rectovaginal fistula (RVF) that was active and considerable in size (measuring 3.5 cm in length and 1 cm in width). Her condition was accompanied by tissue alterations at both the vaginal and rectal openings. Following her admission to our hospital, the patient’s case was discussed during both surgical and multidisciplinary hospital team (IRB) meetings. The team decided to combine a modified Martius flap with autologous MFAT containing MSCs. The results were remarkable, leading to comprehensive anatomical and clinical resolution of the RVF. Equally significant was the improvement in the patient’s overall quality of life and sexual satisfaction during the one-year follow-up period. The integration of the modified Martius flap with MFAT emerges as a highly promising approach for addressing CD-related RVFs that had historically been, and still are, difficult to treat, given their often refractory nature and low healing success rates.

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“…The pool of proliferating APCs is located in the stromovascular fraction of adipose tissue and is a depot and sex-and age-dependent [31,40,45,49,50]. Ex vivo and in vivo human studies have identified APC types among CD31− and CD34+ stromal vascular fractions [51][52][53][54][55][56][57]. Similarly, in a mice-specific sub-population characterized by cell surface immune markers was identified to possess proliferation and differentiation pro-adipogenic functional capabilities [51,53,54].…”

Section: Adipocyte Hyperplasiamentioning

confidence: 99%

Adipose Tissue Hyperplasia and Hypertrophy in Common and Syndromic Obesity—The Case of BBS Obesity

Horwitz

Birk

2023

Nutrients

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Obesity is a metabolic state generated by the expansion of adipose tissue. Adipose tissue expansion depends on the interplay between hyperplasia and hypertrophy, and is mainly regulated by a complex interaction between genetics and excess energy intake. However, the genetic regulation of adipose tissue expansion is yet to be fully understood. Obesity can be divided into common multifactorial/polygenic obesity and monogenic obesity, non-syndromic and syndromic. Several genes related to obesity were found through studies of monogenic non-syndromic obesity models. However, syndromic obesity, characterized by additional features other than obesity, suggesting a more global role of the mutant genes related to the syndrome and, thus, an additional peripheral influence on the development of obesity, were hardly studied to date in this regard. This review summarizes present knowledge regarding the hyperplasia and hypertrophy of adipocytes in common obesity. Additionally, we highlight the scarce research on syndromic obesity as a model for studying adipocyte hyperplasia and hypertrophy, focusing on Bardet–Biedl syndrome (BBS). BBS obesity involves central and peripheral mechanisms, with molecular and mechanistic alternation in adipocyte hyperplasia and hypertrophy. Thus, we argue that using syndromic obesity models, such as BBS, can further advance our knowledge regarding peripheral adipocyte regulation in obesity.

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Medicinal signaling cells niche in stromal vascular fraction from lipoaspirate and microfragmented counterpart

Cited by 4 publications

References 44 publications

Autologous Platelet-Rich Plasma (PRP) for Treating Androgenetic Alopecia: A Novel Treatment Protocol Standardized on 2 Cases

Autologous Platelet-Rich Plasma (PRP) for Treating Androgenetic Alopecia: A Novel Treatment Protocol Standardized on 2 Cases

Novel Approach in Rectovaginal Fistula Treatment: Combination of Modified Martius Flap and Autologous Micro-Fragmented Adipose Tissue

Adipose Tissue Hyperplasia and Hypertrophy in Common and Syndromic Obesity—The Case of BBS Obesity

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