Medicinal plants of the brazilian cerrado: knowing to preserve

Santos, Lucinéia dos; Viel, Amanda Martins; Tarosso, Leandro F.; Momesso, Luciano da Silva; Palmieri, Darío Abel; Spera, Kamille Daleck

doi:10.14393/bj-v36n2a2020-42784

Cited by 5 publications

(3 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Brazil has been designated the world’s richest biodiversity, and Cerrado is considered the second largest biome, exhibiting a vast diversity of natural plants [ 17 , 18 , 19 ]. These plants bring particular properties that allow them to adapt to the hostile environment and the presence of herbivores and pathogens, reflecting the diversity of their secondary compounds [ 20 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

Bio-Prospecting of Crude Leaf Extracts from Thirteen Plants of Brazilian Cerrado Biome on Human Glioma Cell Lines

et al. 2023

View full text Add to dashboard Cite

(1) Background: Malignant gliomas are aggressive tumors characterized by fast cellular growth and highly invasive properties. Despite all biological and clinical advances in therapy, the standard treatment remains essentially palliative. Therefore, searching for alternative therapies that minimize adverse symptoms and improve glioblastoma patients’ outcomes is imperative. Natural products represent an essential source in the discovery of such new drugs. Plants from the cerrado biome have been receiving increased attention due to the presence of secondary metabolites with significant therapeutic potential. (2) Aim: This study provides data on the cytotoxic potential of 13 leaf extracts obtained from plants of 5 families (Anacardiaceae, Annonaceae, Fabaceae, Melastomataceae e Siparunaceae) found in the Brazilian cerrado biome on a panel of 5 glioma cell lines and one normal astrocyte. (3) Methods: The effect of crude extracts on cell viability was evaluated by MTS assay. Mass spectrometry (ESI FT-ICR MS) was performed to identify the secondary metabolites classes presented in the crude extracts and partitions. (4) Results: Our results revealed the cytotoxic potential of Melastomataceae species Miconia cuspidata, Miconia albicans, and Miconia chamissois. Additionally, comparing the four partitions obtained from M. chamissois crude extract indicates that the chloroform partition had the greatest cytotoxic activity against the glioma cell lines. The partitions also showed a mean IC50 close to chemotherapy, temozolomide; nevertheless, lower toxicity against normal astrocytes. Analysis of secondary metabolites classes presented in these crude extracts and partitions indicates the presence of phenolic compounds. (5) Conclusion: These findings highlight M. chamissois chloroform partition as a promising component and may guide the search for the development of additional new anticancer therapies.

show abstract

Section: Introductionmentioning

confidence: 99%

Bio-Prospecting of Crude Leaf Extracts from Thirteen Plants of Brazilian Cerrado Biome on Human Glioma Cell Lines

et al. 2023

View full text Add to dashboard Cite

show abstract

“…Com relação ao Cerrado, revisões recentes (CARVALHO et al, 2019;SANTOS et al, 2020) descrevem uma série de espécies vegetais típicas desse bioma que possuem propriedades medicinais e têm sido utiliza-das tradicionalmente pela população como recurso terapêutico. As diferentes plantas dos referidos estudos se destacam por apresentarem atividades biológicas como antifúngica, antitumoral, diurética, adstringente, antiulcerogênica, anti-inflamatória, hipoglicemiante, e aplicações no tratamento de distúrbios gastrointestinais, além de outros.…”

Section: Introductionunclassified

Metabólitos secundários e potencial tóxico do extrato bruto hidroetanólico das folhas de mangaba - Hancornia speciosa Gomes

Arraes

Junior²,

Habitante

et al. 2022

R. Eletr. Cient. da Uergs

View full text Add to dashboard Cite

O uso de plantas medicinais data de aproximadamente 10 mil anos antes de Cristo. O potencial terapêutico dessas plantas justifica a realização de análises químicas para identificar compostos bioativos responsáveis pela melhora de enfermidades. Investigou-se, neste trabalho, a fitoquímica das folhas de mangaba (Hancornia speciosa Gomes) e avaliou-se seu potencial tóxico. As folhas da planta foram coletadas na Unidade I da UFMT/CUA, em Pontal do Araguaia (MT), e o extrato bruto obtido por maceração em solução etanólica a 70%. A análise fitoquímica do extrato bruto seco obtido foi realizada através de reações químicas clássicas colorimétricas. A investigação do potencial tóxico se deu frente às larvas de Artemia salina Leach, nas concentrações de 15 μg.mL-1, 50 μg.mL-1, 100 μg.mL-1, 500 μg.mL-1, 600 μg.mL-1 e 700 μg.mL-1. A triagem fitoquímica dos metabólitos secundários sinalizou forte presença de catequinas, esteroides, fenóis, flavanonas, flavononóis, saponinas, taninos e taninos catéquicos. Observou-se ainda ausência de uma série de outros metabólitos secundários. A análise do extrato frente às larvas de A. salina, nas diferentes concentrações, revelou atoxicidade (CL50 = 4035,1 μg.mL-1), levantando bons indícios de uma segura utilização desse extrato para fins terapêuticos. Os resultados indicam a presença de metabólitos secundários interessantes da classe dos antioxidantes que podem apresentar diferentes atividades biológicas, sendo que o grupo de pesquisa já se encontra investigando alguns, no sentido da busca de novos fármacos obtidos através de plantas ou frutos, especialmente da região do Médio Araguaia. Palavras-chave: Fitoquímica; planta medicinal; toxicidade. Abstract Secondary metabolites and toxic potential of the crude hydroethanolic extract of mangaba leaves - Hancornia speciosa Gomes The use of medicinal plants dates from approximately 10 thousand years before Christ. The therapeutic potential of these plants justifies the performance of chemical analyzes to identify bioactive compounds responsible for the improvement of diseases. In this work, the phytochemistry of mangaba leaves (Hancornia speciosa Gomes) was investigated and its toxic potential was evaluated. The leaves of the plant were collected at Unit I of UFMT/CUA, in Pontal do Araguaia (MT) and the crude extract was obtained by maceration in 70% ethanolic solution. Phytochemical analysis of the crude dry extract obtained was performed using classical colorimetric chemical reactions. The investigation of the toxic potential was carried out against Artemia salina Leach larvae, at concentrations of 15 μg.mL-1, 50 μg.mL-1, 100 μg.mL-1, 500 μg.mL-1, 600 μg.mL-1 and 700 μg.mL-1. The phytochemical screening of secondary metabolites indicated a strong presence of catechins, steroids, phenols, flavanones, flavononols, saponins, tannins and catechetical tannins. It was also observed the absence of a series of other secondary metabolites. The analysis of the extract against A. salina larvae, at different concentrations, revealed toxicity (LC50 = 4035.1 μg.mL-1), raising good indications of a safe use of this extract for therapeutic purposes. The results indicate the presence of interesting secondary metabolites of the class of antioxidants that can present different biological activities, in which the research group is already investigating some, in the sense of searching for new drugs obtained from plants or fruits, especially from the region of Middle Araguaia. Keywords: Phytochemistry; medicinal plant; toxicity. Resumen Metabolitos secundarios y potencial tóxico del extracto hidroetanólico crudo de hojas de mangaba - Hancornia speciosa Gomes El uso de las plantas medicinales data aproximadamente de 10 mil años antes de Cristo. El potencial terapéutico de estas plantas justifica la realización de análisis químicos para identificar compuestos bioactivos responsables de la mejora de enfermedades. En este trabajo se investigó la fitoquímica de las hojas de mangaba (Hancornia speciosa Gomes) y se evaluó su potencial tóxico. Las hojas de la planta fueron recolectadas en la Unidad I de la UFMT/CUA, en Pontal do Araguaia (MT), y el extracto crudo fue obtenido por maceración en solución etanólica al 70%. El análisis fitoquímico del extracto seco crudo obtenido se realizó mediante reacciones químicas colorimétricas clásicas. La investigación del potencial tóxico se realizó en larvas de Artemia salina Leach, en concentraciones de 15 μg.mL-1, 50 μg.mL-1, 100 μg.mL-1, 500 μg.mL-1, 600 μg.mL-1 y 700 μg.mL-1. El tamizaje fitoquímico de metabolitos secundarios indicó una fuerte presencia de catequinas, esteroides, fenoles, flavanonas, flavononoles, saponinas, taninos y taninos de catequinas. También se observó la ausencia de una serie de otros metabolitos secundarios. El análisis del extracto en larvas de A. salina, a diferentes concentraciones, no reveló toxicidad (CL50 = 4035,1 μg.mL-1), lo que plantea buenos indicios de un uso seguro de este extracto con fines terapéuticos. Los resultados indican la presencia de interesantes metabolitos secundarios de la clase de los antioxidantes que pueden presentar diferentes actividades biológicas, siendo que el grupo de investigación ya investiga algunos, en el sentido de buscar nuevos fármacos obtenidos de plantas o frutos, especialmente de la región de Medio Araguaia. Palabras clave: Fitoquímica; planta medicinal; toxicidad.

show abstract

“…Because of the dry climate with seasonal rain, this biome presents characteristic upland vegetation on deep and well-drained soils [ 13 ]. The trees, shrubs, and herbaceous plants have characteristics unique to their adaptation, such as the production of secondary metabolites that protect against herbivory and pathogens [ 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

Bioprospecting of Natural Compounds from Brazilian Cerrado Biome Plants in Human Cervical Cancer Cell Lines

Rosa

Silva

Longato

et al. 2021

IJMS

View full text Add to dashboard Cite

Cervical cancer is the third most common in Brazilian women. The chemotherapy used for the treatment of this disease can cause many side effects; then, to overcome this problem, new treatment options are necessary. Natural compounds represent one of the most promising sources for the development of new drugs. In this study, 13 different species of 6 families from the Brazilian Cerrado vegetation biome were screened against human cervical cancer cell lines (CCC). Some of these species were also evaluated in one normal keratinocyte cell line (HaCaT). The effect of crude extracts on cell viability was evaluated by a colorimetric method (MTS assay). Extracts from Annona crassiflora, Miconia albicans, Miconia chamissois, Stryphnodendron adstringens, Tapirira guianensis, Xylopia aromatica, and Achyrocline alata showed half-maximal inhibitory concentration (IC50) values < 30 μg/mL for at least one CCC. A. crassiflora and S. adstringens extracts were selective for CCC. Mass spectrometry (Electrospray Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometer (ESI FT-ICR MS)) of A. crassiflora identified fatty acids and flavonols as secondary compounds. One of the A. crassiflora fractions, 7C24 (from chloroform partition), increased H2AX phosphorylation (suggesting DNA damage), PARP cleavage, and cell cycle arrest in CCC. Kaempferol-3-O-rhamnoside and oleic acid were bioactive molecules identified in 7C24 fraction. These findings emphasize the importance of investigating bioactive molecules from natural sources for developing new anti-cancer drugs.

show abstract

Medicinal plants of the brazilian cerrado: knowing to preserve

Cited by 5 publications

References 23 publications

Bio-Prospecting of Crude Leaf Extracts from Thirteen Plants of Brazilian Cerrado Biome on Human Glioma Cell Lines

Bio-Prospecting of Crude Leaf Extracts from Thirteen Plants of Brazilian Cerrado Biome on Human Glioma Cell Lines

Metabólitos secundários e potencial tóxico do extrato bruto hidroetanólico das folhas de mangaba - Hancornia speciosa Gomes

Bioprospecting of Natural Compounds from Brazilian Cerrado Biome Plants in Human Cervical Cancer Cell Lines

Contact Info

Product

Resources

About