Medicinal Mushrooms: Cultivation and Pharmaceutical Impact

Cheong, Peter Chiew Hing; Tan, Cher Siang; Fung, Shin Yee

doi:10.1007/978-3-030-02622-6_14

Cited by 4 publications

(5 citation statements)

References 79 publications

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“…The literature describes that the combination of yeast extract, peptone and glucose in a 1:1:4 ratio has a positive effect on the cell growth of the mushroom G. lucidum (Cheong et al, 2018). This proportion is similar to that used in experiment 4 (E4A and E4NA), which present one of the highest yields of mycelial biomass.…”

Section: Discussionmentioning

confidence: 61%

Production of mycelial biomass, proteases and protease inhibitors by Ganoderma lucidum under different submerged fermentation conditions

et al. 2023

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Ganoderma lucidum is a medicinal mushroom widely recognized as a source of biomolecules with pharmacological properties, however, little is known about the factors that influence the synthesis of bioactive proteins by this fungus when cultivated under submerged fermentation. The objective of this work was to evaluate the production of mycelial biomass and intracellular proteases and protease inhibitors by G. lucidum cultivated under different submerged fermentation conditions. The cultivation was carried out in a medium composed of glucose (10 or 20 g.L-1), soy peptone (2.5 or 5 g.L-1) and yeast extract (5 g.L-1), with incubation under agitation (120 rpm) and non-agitation, totaling 8 experimental conditions. Biomass production was determined from the dry weight, while glucose consumption was estimated by quantification of reducing sugars. The proteins were extracted in NaCl (0.15 M), and the protein extracts were submitted to protein quantification by the Bradford method, total proteolytic activity using azocasein, caseinolytic and fibrinolytic activity in Petri dishes, activity of serine (trypsin and chymotrypsin) and cysteine (papain) protease inhibitors. Cultivation in agitated condition showed higher biomass production with a maximum value of 7 g.L-1, in addition to higher activities of trypsin, chymotrypsin and papain inhibitors, with 154 IU.mg-1, 153 IU.mg-1 e 343 IU.mg-1 of protein, respectively. The non-agitated condition showed a greater potential for obtaining proteins, total proteases, caseinolytic and fibrinolytic enzymes, with maximum values of 433 mg.g-1 of extract, 71 U.mL-1 of extract, 63.62 mm2 and 50.27 mm2, respectively. Thus, a medium composed of soy peptone, yest extract and glucose in a 1:2:4 proportion is recommended, under agitation to produce protease inhibitors, and the non-agitated condition when the target is, mainly caseinolytic and fibrinolytic enzymes.

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Section: Discussionmentioning

confidence: 61%

Production of mycelial biomass, proteases and protease inhibitors by Ganoderma lucidum under different submerged fermentation conditions

et al. 2023

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“…The medium should be cooled down gently after the sterilization since the sterilized medium is easy to be contaminated by the microbes in returning current. 5. Spawning (Inoculation): There are two types of spawning, i. e., one by solid spawn (Fig.…”

Section: Coolingmentioning

confidence: 99%

“…are similar as those of edible mushrooms [3]. Other famous medicinal mushrooms including entomopathogenic fungi are also cultivated by very classical and simple technology based on the cultivation technology of edible mushrooms, except for the modification of substrates constituents [4,5]. In the following, we would like to introduce each process of mushroom cultivation and their physiological background, based on edible mushroom models, in order to find new idea to improve the mushroom production.…”

Section: Introductionmentioning

confidence: 99%

Technology of Mushroom Cultivation

Suzuki

2019

JST

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Mushroom cultivation has been developed by two ways, i. e., one based on cultivation by wood-log and another based on cultivation by non-wood-log. Non-wood-log cultivation would be categorized into two aspects. Mushroom cultivation under non-aseptic condition, i. e., one characterized by the processes of fermentation (composting) and casing, and another characterized by using sterilized media followed by cultivation process associated with strict control of environmental factors such as light, temperature, humidity, and CO2 concentration.

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“…and Thitarodes spp. During autumn, the fungus attacks the larvae approximately 15 cm underground, gradually multiplies within its entire body, and nally by the summer of the second year (May-June) the larvae die and fruiting bodies protrude from the head of larvae [12,13,14]. Similarly, C. militaris, a scorpion that attacks soil-dwelling lepidopteran larvae and pupae in light forests, has two parts: stalk (fruit body) and sclerotium (dead part of insect) [11], and is somewhat similar to C. sinensis, hence is known as orange C sinensis [8].…”

Section: Introductionmentioning

confidence: 99%

“…Similarly, C. militaris, a scorpion that attacks soil-dwelling lepidopteran larvae and pupae in light forests, has two parts: stalk (fruit body) and sclerotium (dead part of insect) [11], and is somewhat similar to C. sinensis, hence is known as orange C sinensis [8]. But due to the limited production, endemic occurrence, unique growth pattern, biological life cycle, long-term symbiotic and time-consuming association between the partners, harvesting process, and enormous pharmaceutical application results in a very high price for these mushrooms [13,14] Cordycepin is an adenosine derivative, a major bioactive constituent of C. militaris that was rst isolated in 1951 by Cunningham and his co-workers [15], having the molecular structure of 9-(3-deoxy-β-Dribofuranosyl) adenine [2,16]. This compound lacks one oxygen molecule at the third carbon of ribose sugar which differs it from adenosine nucleotide [17].…”

Section: Introductionmentioning

confidence: 99%

Cordycepin: A Molecular Trojan Horse Against Fusarium oxysporum f. sp. cubense - A Computational Perspective

Showkat,

Nagesha,

Ashraf

et al. 2024

Preprint

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Fusarium oxysporum is a notorious plant pathogen responsible for devastating losses in agricultural and horticultural crops worldwide. To combat the economic and ecological challenges posed by this pathogen, there is a pressing need for novel antifungal agents. Cordycepin, a natural compound derived from Cordyceps species, has exhibited promising antifungal properties against various pathogens. In this study, we employed molecular docking techniques to investigate the potential inhibitory activity of cordycepin against three critical proteins in Fusarium oxysporum f. sp. cubense (Foc) SIX13, Fosp9, and FocTR4. Through computational analysis, we elucidated the binding interactions between cordycepin and these target proteins. Our results revealed that cordycepin can effectively dock into the active sites of SIX13, Fosp9, and FocTR4, forming stable complexes with favorable binding energies. These interactions involve key amino acid residues crucial for the biological function of these proteins. Furthermore, we explored the binding modes and potential hydrogen bond interactions, shedding light on the mechanisms through which cordycepin may disrupt the function of these vital proteins in Fusarium oxysporum. These findings provide valuable insights into the potential of cordycepin as a novel antifungal agent targeting specific proteins in Fusarium oxysporum, which could pave the way for the development of innovative strategies for managing this devastating plant pathogen and mitigating its impact on global agriculture. Further experimental validation and in vivo studies are warranted to confirm the therapeutic potential of cordycepin in controlling Fusarium oxysporum infections.

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Medicinal Mushrooms: Cultivation and Pharmaceutical Impact

Cited by 4 publications

References 79 publications

Production of mycelial biomass, proteases and protease inhibitors by Ganoderma lucidum under different submerged fermentation conditions

Production of mycelial biomass, proteases and protease inhibitors by Ganoderma lucidum under different submerged fermentation conditions

Technology of Mushroom Cultivation

Cordycepin: A Molecular Trojan Horse Against Fusarium oxysporum f. sp. cubense - A Computational Perspective

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