Medicinal mushroom science: Current perspectives, advances, evidences, and challenges

Wasser, Solomon P.

doi:10.4103/2319-4170.138318

Cited by 284 publications

(214 citation statements)

References 71 publications

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“…Similarly, medicinal fungi generate billions of dollars worth of products and services (Chang and Wasser 2012;Wasser 2014). Globally, about 10 000 species of fleshy mushrooms have been described .…”

Section: Edible and Medicinal Mushroomsmentioning

confidence: 99%

Fungal DNA barcoding

2016

Genome

209

149

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Fungi are ubiquitous in both natural and human-made environments. They play important roles in the health of plants, animals, and humans, and in broad ecosystem functions. Thus, having an efficient species-level identification system could significantly enhance our ability to treat fungal diseases and to monitor the spatial and temporal patterns of fungal distributions and migrations. DNA barcoding is a potent approach for rapid identification of fungal specimens, generating novel species hypothesis, and guiding biodiversity and ecological studies. In this mini-review, I briefly summarize (i) the history of DNA sequence-based fungal identification; (ii) the emergence of the ITS region as the consensus primary fungal barcode; (iii) the use of the ITS barcodes to address a variety of issues on fungal diversity from local to global scales, including generating a large number of species hypothesis; and (iv) the problems with the ITS barcode region and the approaches to overcome these problems. Similar to DNA barcoding research on plants and animals, significant progress has been achieved over the last few years in terms of both the questions being addressed and the foundations being laid for future research endeavors. However, significant challenges remain. I suggest three broad areas of research to enhance the usefulness of fungal DNA barcoding to meet the current and future challenges: (i) develop a common set of primers and technologies that allow the amplification and sequencing of all fungi at both the primary and secondary barcode loci; (ii) compile a centralized reference database that includes all recognized fungal species as well as species hypothesis, and allows regular updates from the research community; and (iii) establish a consensus set of new species recognition criteria based on barcode DNA sequences that can be applied across the fungal kingdom.Key words: ITS, secondary barcode, herbarium, metabarcoding, operational taxonomic unit. Résumé :Les champignons sont présents partout, tant dans les environnements naturels qu'anthropisés. Ils jouent des rôles importants dans la santé des plantes, des animaux et des humains de même que dans les écosystèmes. Ainsi, la disponibilité d'un système efficace d'identification des espèces pourrait grandement augmenter la capacité de traiter les maladies fongiques et de surveiller les variations spatiales et temporelles dans la distribution et la migration des champignons. Le codage à barres de l'ADN est une approche potentiellement puissante pour l'identification rapide de spécimens fongiques, permettant d'identifier de nouvelles espèces et guidant les études sur la biodiversité et les écosystèmes. Dans cette mini-synthèse, l'auteur résume brièvement (i) l'histoire de l'identification des champignons sur la base de séquences d'ADN; (ii) l'émergence de la région ITS en tant que code à barre fongique primaire consensuel; (iii) l'emploi des codes à barres ITS pour étudier nombre de questions sur la diversité des champignons de l'échelle locale à globale, inc...

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Section: Edible and Medicinal Mushroomsmentioning

confidence: 99%

Fungal DNA barcoding

2016

Genome

209

149

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“…A great diversity of living organisms can be used for the improvement of human health, such as biologically active components extracted from the plants, mushrooms and lichens [1][2][3]. In searching for new therapeutic alternatives, many different lichen species were investigated, due to significant antiviral, antimicrobial, anticancer, antihyperglycemic, cardioprotective, antiparasitic, anti-inflammatory and antibiotic effects that they have exhibited so far [3][4][5].…”

Section: Introductionmentioning

confidence: 99%

Chemical composition and bioactive properties of the lichen, <i>Pleurosticta acetabulum</i >

Tomović¹,

Kosanić²,

Ristić³

et al. 2018

Trop. J. Pharm Res

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“…crispa -цінний їстівний лікарський гриб, відомий у медичній практиці як «гриб-баран» (Wasser 2010(Wasser , 2014Choi et al, 2013;Hu et al, 2016). У народній медицині східних слов'ян настої його плодових тіл застосовували при лікуванні захворювань печінки та жовчного міхура .…”

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Морфолого-культуральні властивості рідкісного лікарського гриба Sparassis crispa (Sparassidaceae, Polyporales)

Mykchaylova¹,

Gryganskyi²,

Lomberg³

et al. 2017

Ukr J Ecol

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A verification of Sparassis crispa (Wulfen) Fr. strains of the IBK Mushroom Culture Collection using molecular-genetic and cultural-morphological methods were done. For all S. crispa strains a complete determination of the nucleotide sequences of the internal transcribed spacer: ITS1, 5.8S and ITS2 regions of rRNA, as well as the partial determination of 18S and 28S sequences surrounding the ITS, was performed. As a result of the search in the gene bank, the S. crispa samples deposited there showed 99-98% identity with the sequences we received, thus confirming the species of the examined strains. On the basis of the sequences we received, investigated strains were registered in NCBI GenBank. To confirm the taxonomic affiliation of the strains we studied their cultural and morphological characteristics. Microstructures of vegetative mycelium by optical and scanning electron microscopy were investigated. In all S. crispa cultures we observed the hyphae with regular one-sided gapless clamp connections, numerous secretory cells on the surface of the hyphae, anastomoses, filamentous strands and films. We studied the growth rate and morphology of the strains on seven agar nutrient media. According to the radial growth rate of S. crispa cultures can be placed to the very slowly growing mushrooms, growth rate of 0,5−2,8 mm/day. We found the selective media such as malt agar with the addition of pine sawdust (SS) and larch sawdust (SM) as most favorable for the vegetative growth and generative stage for all strains, temperature of incubation was 26 ± 0.1 °C. For mycelial growth the critical temperature was 39 ± 0.1 °C for S. crispa strains 312, 314 and 40 ± 0.1 °C for the strains 304, 2004. We confirmed belonging of studied strains to S. сrispa species according to established morphological and cultural characteristics. This result coincided with the results of DNA typing. Thus, the obtained mycelium growth parameters on nutrient media, micro-and macromorphological characteristics can be used as additional taxonomic characteristics of S. сrispa culture in the vegetative stage of growth. The strain S. сrispa 314 may become a potential producer for new fungal biotechnologies in Ukraine in the near future and reintroduction of this species in the nature.

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Medicinal mushroom science: Current perspectives, advances, evidences, and challenges

Cited by 284 publications

References 71 publications

Fungal DNA barcoding

Fungal DNA barcoding

Chemical composition and bioactive properties of the lichen, <i>Pleurosticta acetabulum</i >

Морфолого-культуральні властивості рідкісного лікарського гриба Sparassis crispa (Sparassidaceae, Polyporales)

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