The rampant practice of traditional medicine in the big cities in Java can not be separated from the influence of globalization. It is advertised through flyers, pamphlets, signage, television and internet. Medical practice can not be separated from the mixture of elements of the local culture, external/ foreign as well as modern practice medical later adopted in order to enhance public interest in their treatment. The purpose of this article is to analyze the practice of acculturation in traditional medicine related to methods, tools, advertising and meaning behind the ways the adoption of other culture into the traditional practice. An integrative ethnographic study was conducted in Bantul and Sleman, Yogyakarta, on traditional healers such as gurah, bekam, metaphysical/ spiritual, herbal, traditional massage therapy, as well as hemorrhoids. Other information was obtained from interviews with the patients. Medical pluralism by wrapping traditional therapy combined with health culture from the outside attracts consumers with a dogmatic way was made by Javanese traditional healers. They advertise with testimony on local television with the aim of introducing methods, tools, disease cure rates, so the number of patients increases. Acculturation appears that more and more people believe in the business practices and their treatment.