Medical Parasitology

Markell, Edward K.; Voge, Marietta; L, Abad Polo

doi:10.1097/00000441-196508000-00047

Cited by 20 publications

(34 citation statements)

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“…African sleeping sickness is found in many regions of Africa, caused by either T brucei gambiense or T brucei rhodesiense, whereas in cattle, 7: congolense causes the corresponding disease known as nagana. Leishmania are widely spread in nature, causing a variety of diseases, including human visceral leishmaniasis (kala azar, L. donovani), human cutaneous leishmaniasis (oriental sore, L. tropica), and mucocutaneous leishmaniasis (espundia, L. braziliensis) (Markell & Voge, 1981). The enzymes that effect the synthesis of TSH in these pathogenic organisms represent important and potentially specific targets for the development of antiparasitic chemotherapy.…”

Section: Discussionmentioning

confidence: 99%

Purification of glutathionylspermidine and trypanothione synthetases from crithidia fasciculata

et al. 1992

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Two enzymes involved in the biosynthesis of the trypanosomatid-specific dithiol trypanothione-glutathionylspermidine (Gsp) synthetase and trypanothione (TSH) synthetase-have been identified and purified individually from Crithidia fusciculutu. The Gsp synthetase has been purified 93-fold and the TSH synthetase 52-fold to apparent homogeneity from a single DEAE fraction that contained both activities. This constitutes the first indication that the enzymatic conversion of two glutathione molecules and one spermidine to the N ' , NE-bis(glutathiony1)spermidine (TSH) occurs in two discrete enzymatic steps. Gsp synthetase, which has a kc,, of 600/min, shows no detectable TSH synthetase activity, whereas TSH synthetase does not make any detectable Gsp and has a kc,, of 75/min. The 90-kDa Gsp synthetase and 82-kDa TSH synthetase are separable on phenyl Superose and remain separated on gel filtration columns in high salt (0.8 M NaCl). Active complexes can be formed under low to moderate salt conditions (0.0-0.15 M NaCl), consistent with a functional complex in vivo.Keywords: amide-forming synthetases; glutathione metabolism; glutathionylspermidine synthetase; polyamine metabolism; trypanothione synthetase The polyamines (putrescine, spermidine, and spermine) and GSH are found in millimolar concentrations in most biological systems. They are thought to be involved in cell growth and differentiation and regulation of important enzymatic processes. GSH plays an important role in the management of oxidative stress and in the maintenance and the regulation of intracellular thiol/disulfide redox balance. Polyamines are required for optimal growth in many cell types, although their precise regulatory functions remain obscure (Tabor & Tabor, 1984). The majority of intracellular GSH of the trypanosomatid parasites,

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Section: Discussionmentioning

confidence: 99%

Purification of glutathionylspermidine and trypanothione synthetases from crithidia fasciculata

et al. 1992

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“…Each stool sample was analysed 1-10 h after collection using the techniques of Faust and Kato Katz according to international recommendations (WHO, 1991;Markell and Voge, 1992). The intensity of infection was indirectly estimated by counting the number of eggs per gram of faeces (epg) (Stephenson and Holland, 1987).…”

Section: Methodsmentioning

confidence: 99%

Trichuriasis and low-iron status in schoolchildren from Northwest Mexico

et al. 2010

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Background/Objectives: The aim of this study was to investigate the association between trichuriasis and iron status in rural schoolchildren from Northwest Mexico. Subjects/Methods: A total of 73 schoolchildren (37 boys, 36 girls) between the ages of 6 and 10 years were voluntarily recruited from the public primary school of the rural community of El Higueral in Culiacan, Sinaloa (Northwest Mexico) for a cross-sectional study with a longitudinal follow-up of 5 weeks. Data were collected on socioeconomic status, anthropometry, haematological and biochemical indices of iron status, daily iron intake, and prevalence and intensity of trichuriasis. Multiple regression models, independent t-test and paired t-test were used to analyse the association between trichuriasis and iron status in cross-sectional and longitudinal samples, respectively. Adjusted models were tested for linear regression assumptions using residual plots. Results: The mean age of the Trichuris-free and Trichuris-infected groups was 7.7±1.3 and 7.7±1.5 years respectively (P ¼ 0.92). The height for age was significantly higher in the Trichuris-free group than the Trichuris-infected group (P ¼ 0.02). No differences were found in the socioeconomic variables between the two groups. At baseline, significantly higher concentrations of haemoglobin, haematocrit, blood cell count (RBC) and serum iron were measured in the Trichuris-free group compared with the Trichuris-infected children (Po0.05). An association was found between trichuriasis and haemoglobin adjusted for socioeconomic variables, age and sex. Haemoglobin, RBC and serum ferritin concentrations were significantly increased in the infected children 5 weeks after treatment (Po0.05). Conclusions: Trichuriasis could be a risk factor for low-iron status in the schoolchildren of Northwest Mexico.

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“…Las características de los pacientes (mujer de 25 años; varón de 15 años) indican que la edad y el sexo en las infestaciones es variado, al igual que lo registrado a nivel mundil 1,2,4,11 . Los síntomas de las myiasis son inespecíficos y pueden confundirse con otros disturbios del tubo digestivo ocasionados por virosis, bacteriosis o intoxicaciones 8 . Sus aspectos clínicos varían de acuerdo al número de huevos o larvas tragadas, al órgano afectado y a la especie de mosca involucrada 8 .…”

Section: Miasis Consumo Doméstico De áGua Eristalis Tenaxunclassified

“…Los síntomas de las myiasis son inespecíficos y pueden confundirse con otros disturbios del tubo digestivo ocasionados por virosis, bacteriosis o intoxicaciones 8 . Sus aspectos clínicos varían de acuerdo al número de huevos o larvas tragadas, al órgano afectado y a la especie de mosca involucrada 8 . En general, los pacientes concurren a Figura-Larva de Eristalis tenax recuperada de heces humanas.…”

Section: Miasis Consumo Doméstico De áGua Eristalis Tenaxunclassified

Myiasis gastrointestinal humana por Eristalis tenax

1998

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Son caracterizadas las myiasis registradas en Bariloche y establecidas las condiciones probables bajo las cuales se produjeron las infestaciones. Las larvas obtenidas a partir de heces de 2 pacientes fueron identificadas como Eristalis tenax (Diptera: Syrphidae) de acuerdo a las claves de Hartley (1961) y Organización Panamericana de la Salud (1962). Estos 2 casos de myiasis gastrointestinal humana constituyen los primeros registrados en Bariloche (Patagonia, Argentina) y sus características responden a las registradas para esta especie de Díptera en otras partes del mundo. La falta de control específico en el sistema domiciliario de suministro de agua ha sido la causa más probable de la infestación. Este registro extiende la distribución de E. tenax y de las myiasis gastrointestinales humanas en América del Sur hasta los 41º 03' S.

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Medical Parasitology

Cited by 20 publications

References 0 publications

Purification of glutathionylspermidine and trypanothione synthetases from crithidia fasciculata

Purification of glutathionylspermidine and trypanothione synthetases from crithidia fasciculata

Trichuriasis and low-iron status in schoolchildren from Northwest Mexico

Myiasis gastrointestinal humana por Eristalis tenax

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