Medical ozone therapy reduces oxidative stress and intestinal damage in an experimental model of necrotizing enterocolitis in neonatal rats

Güven, Ahmet; Gündoğdu, Gökhan; Vurucu, Sebahattin; Uysal, Bülent; Öztaş, Emin; Öztürk, Haluk; Korkmaz, Ahmet

doi:10.1016/j.jpedsurg.2009.01.007

Cited by 62 publications

(44 citation statements)

References 42 publications

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“…[4][5][6] A gas mixture comprising ozone/oxygen used in medicine is known as medical ozone therapy. It was demonstrated that ozone increases antioxidant enzyme activities such as glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and catalase, preparing the host to face physiopathologic conditions mediated by reactive oxygen species (ROS), [7][8][9] as reported in our previous works. 7,10 Clinically important beneficial effects of ozone therapy in some disorders such as peritonitis, infected wounds, skin ulcers, and gangrenes have been reported.…”

Section: Introductionmentioning

confidence: 66%

“…It was demonstrated that ozone increases antioxidant enzyme activities such as glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and catalase, preparing the host to face physiopathologic conditions mediated by reactive oxygen species (ROS), [7][8][9] as reported in our previous works. 7,10 Clinically important beneficial effects of ozone therapy in some disorders such as peritonitis, infected wounds, skin ulcers, and gangrenes have been reported. 8,9 In this study we hypothesized that the ozone therapy may have protective effects in rats with APAP-induced nephrotoxicity by improving antioxidant activity.…”

Section: Introductionmentioning

confidence: 66%

“…H 2 O 2 , an essential ROS molecule, is able to act as an ozone messenger for eliciting several biological and therapeutic effects. 7,8,10,21 The dogma that H 2 O 2 is always harmful has been changed because, in physiological amounts, it behaves as a regulator of signal transduction and is an important mediator of host defense and immune responses. 9,21 While H 2 O 2 acts immediately and disappears (early and short-acting messenger), LOPs, via the circulation, distribute throughout the tissues and become late and long-lasting messengers.…”

Section: Discussionmentioning

confidence: 99%

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Effects of medical ozone therapy on acetaminophen-induced nephrotoxicity in rats

et al. 2010

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Acetaminophen (APAP), also known as paracetamol, is the commonest cause of toxic ingestion in the world. Because overdose of APAP has life-threatening effects on kidney, treatment of APAP-induced nephrotoxicity has life-saving importance. Aim of the study was to evaluate the efficacy of medical ozone therapy in experimental model of APAP toxication. Twenty-one male Wistar rats (200-250 g) were randomly assigned into three groups containing seven rats each: Sham, control (only APAP treated), and APAP + ozone therapy groups. Rats were killed 48 hours after administration of APAP. Urea, creatinine levels in the blood, and malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) activity in renal tissue were measured. Kidney tissues were stained with hematoxylin and eosin for histological assessment. APAP administration deteriorated the renal functions and significantly elevated renal MDA levels and depleted SOD and GSH-Px activities. Ozone therapy significantly reduced the MDA level, increased the SOD and GSHPx activities, and normalized the renal histology. In conclusion, our study results are consistent with encouraging data for ozone therapy on APAP-induced nephrotoxicity in rats by improving antioxidant mechanism and oxidative stress.

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Section: Introductionmentioning

confidence: 66%

Section: Introductionmentioning

confidence: 66%

Section: Discussionmentioning

confidence: 99%

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Effects of medical ozone therapy on acetaminophen-induced nephrotoxicity in rats

et al. 2010

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“…Under physiological conditions, NO maintains vascular tone and inhibits aggregation and adhesion of neutrophils and platelets to vascular endothelium; these are beneficial aspects of NO function. 8,16,35 NO-induced IRI may also be a result of production of ONOO -, since NO couples with O…”

Section: Mda (Nmol/g-protein)mentioning

confidence: 99%

Beneficial Effects ofN-Acetylcysteine and Ebselen on Renal Ischemia/Reperfusion Injury

et al. 2011

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Introduction: It has been demonstrated that peroxynitrite accompanies acute renal ischemia and contributes to the pathophysiology of renal damage. Therefore, we aimed to investigate the roles of N-acetylcysteine (NAC), a well-known powerful antioxidant, and ebselen (E), a scavenger of peroxynitrite, on renal injury induced by renal ischemia/reperfusion injury (IRI) of rat kidney. Materials and methods: Forty male Sprague-Dawley rats were divided into five groups: sham, renal IRI, renal IRI+NAC, renal IRI+E, and renal IRI+NAC+E. IR injury was induced by 60 min of bilateral renal ischemia followed by 6 h of reperfusion. After reperfusion, kidneys and blood samples were obtained for histopathological and biochemical evaluations. Results: Renal IR resulted in increased malondialdehyde and nitrite/nitrate levels suggesting increased lipid peroxidation and peroxynitrite production and decreased superoxide dismutase and glutathione peroxidase activities. Both NAC and E alone significantly decreased malondialdehyde and nitrite/nitrate levels and increased superoxide dismutase and glutathione peroxidase activities. Additionally in the renal IRI+NAC+E group, all biochemical results were quite close to those of sham group. Histopathologically, the kidney injury in rats treated with combination of NAC and E was found significantly less than the other groups. Conclusions: Both NAC and E are able to ameliorate IRI of the kidney by decreasing oxidative and nitrosative stresses and increasing free radical scavenger properties. Additionally, combination of NAC and E prevents kidney damage more than when each drug is used alone, suggesting that scavenging peroxynitrite nearby antioxidant activity is important in preventing renal IRI.

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“…A gas mixture comprising no less than 95% oxygen and no more than 5% ozone used in medicine is known as medical ozone therapy (Bocci, 2006;Guven et al, 2009). When the gas mixture composed of 95% oxygen and of no more than 5% ozone, is mixed ex vivo, a complex series of physical and chemical processes occur: oxygen dissolved in plasmatic water, almost instantaneously saturates hemoglobin to form oxyhemoglobin and the pO2 increases far higher than physiological level while pCO2 and pH remains fairly constant (Bocci, 1996).…”

Section: Ozone: Its General Effects and Use In Medicinementioning

confidence: 99%

How Ozone Treatment Affects Erythrocytes

Aydoğan¹,

Artis²

2012

Hemodynamics - New Diagnostic and Therapeutic Approaches

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Medical ozone therapy reduces oxidative stress and intestinal damage in an experimental model of necrotizing enterocolitis in neonatal rats

Cited by 62 publications

References 42 publications

Effects of medical ozone therapy on acetaminophen-induced nephrotoxicity in rats

Effects of medical ozone therapy on acetaminophen-induced nephrotoxicity in rats

Beneficial Effects ofN-Acetylcysteine and Ebselen on Renal Ischemia/Reperfusion Injury

How Ozone Treatment Affects Erythrocytes

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