Medicago truncatula root developmental changes by growth-promoting microbes isolated from Fabaceae, growing on organic farms, involve cell cycle changes and WOX5 gene expression

Kępczyńska, Ewa; Karczyński, Piotr

doi:10.1007/s00425-019-03300-5

Cited by 7 publications

(20 citation statements)

References 68 publications

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“…Microalgae application could be expected to directly impact shoot and root elongation since eukaryotic green microalgae produce auxins and cytokinins. Our results are consistent with what has been reported on auxin producing microorganisms such as bacteria and endophytic fungi, which were found to promote plant growth [18,[70][71][72][85][86][87][88]. The difference observed in the different algae treatments could be attributed to the ratios of auxins/cytokinins or different concentrations of specific phytohormones.…”

Section: Discussionsupporting

confidence: 92%

“…The vast majority of studies of growth promotion in M. truncatula and its relatives such as M. sativa concentrate on the effects of growth-promoting bacteria [18,[70][71][72]. These studies especially focus on the mechanisms of these microorganisms in root development and nodulation but pay little attention to the plant architecture and leaf morphology.…”

Section: Discussionmentioning

confidence: 99%

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Strain-Specific Biostimulant Effects of Chlorella and Chlamydomonas Green Microalgae on Medicago truncatula

Gitau

Farkas

Balla

et al. 2021

Plants

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Microalgae have been identified to produce a plethora of bioactive compounds exerting growth stimulating effects on plants. The objective of this study was to investigate the plant-growth-promoting effects of three selected strains of eukaryotic green microalgae. The biostimulatory effects of two Chlorella species (MACC-360 and MACC-38) and a Chlamydomonas reinhardtii strain (cc124) were investigated in a Medicago truncatula model plant grown under controlled greenhouse conditions. The physiological responses of the M. truncatula A17 ecotype to algal biomass addition were characterized thoroughly. The plants were cultivated in pots containing a mixture of vermiculite and soil (1:3) layered with clay at the bottom. The application of live algae cells using the soil drench method significantly increased the plants’ shoot length, leaf size, fresh weight, number of flowers and pigment content. For most of the parameters analyzed, the effects of treatment proved to be specific for the applied algae strains. Overall, Chlorella application led to more robust plants with increased fresh biomass, bigger leaves and more flowers/pods compared to the control and Chlamydomonas-treated samples receiving identical total nutrients.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Strain-Specific Biostimulant Effects of Chlorella and Chlamydomonas Green Microalgae on Medicago truncatula

Gitau

Farkas

Balla

et al. 2021

Plants

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“…Paenibacillus has also been reported as a nodule endophyte in L. albus [ 87 ]. Bacteria isolated from L. luteus and L. angustifolius have been reported to cluster with strains in the genera Rahnella, Serratia, Raoultella, and Stenotrophomonas [ 88 ]. Most of the endophytes isolated from L. mutabilis nodules belong to the family Bacillaceae [ 89 ].…”

Section: Rhizobia That Nodulate Lupins Many More Than Initially Expectedmentioning

confidence: 99%

Lupin, a Unique Legume That Is Nodulated by Multiple Microsymbionts: The Role of Horizontal Gene Transfer

Msaddak

Mars

Quiñones

et al. 2023

IJMS

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Lupin is a high-protein legume crop that grows in a wide range of edaphoclimatic conditions where other crops are not viable. Its unique seed nutrient profile can promote health benefits, and it has been proposed as a phytoremediation plant. Most rhizobia nodulating Lupinus species belong to the genus Bradyrhizobium, comprising strains that are phylogenetically related to B. cytisi, B. hipponenese, B. rifense, B. iriomotense/B. stylosanthis, B. diazoefficiens, B. japonicum, B. canariense/B. lupini, and B. retamae/B. valentinum. Lupins are also nodulated by fast-growing bacteria within the genera Microvirga, Ochrobactrum, Devosia, Phyllobacterium, Agrobacterium, Rhizobium, and Neorhizobium. Phylogenetic analyses of the nod and nif genes, involved in microbial colonization and symbiotic nitrogen fixation, respectively, suggest that fast-growing lupin-nodulating bacteria have acquired their symbiotic genes from rhizobial genera other than Bradyrhizobium. Horizontal transfer represents a key mechanism allowing lupin to form symbioses with bacteria that were previously considered as non-symbiotic or unable to nodulate lupin, which might favor lupin’s adaptation to specific habitats. The characterization of yet-unstudied Lupinus species, including microsymbiont whole genome analyses, will most likely expand and modify the current lupin microsymbiont taxonomy, and provide additional knowledge that might help to further increase lupin’s adaptability to marginal soils and climates.

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“…We had previously identified, and registered in GenBank, 33 PGPR strains including Pseudomonas fluorescens (Ms9N) and Stenotrophomonas maltophilia (Ll4) from the rhizosphere and nodules of alfalfa, barrel medic ( Medicago truncatula ), bean and lupin. We characterized them and showed their growth-promoting effect on Medicago truncatula (Kisiel and Kępczyńska 2016 ; Kępczyńska and Karczyński 2020 ). To our knowledge, effects of these bacteria on in vitro growth of soil-borne Fusarium fungi have not been investigated yet.…”

Section: Introductionmentioning

confidence: 99%

“…Hence, identification of any new potential rhizobacterial isolate and understanding its direct and indirect biocontrol activity is a contribution to sustainable agriculture (Meena et al 2020 ). Here, we broaden the understanding of the molecular roots-to-leaves response in M. truncatula as a consequence of soil treatment with suspensions of Pseudomonas fluorescens (Ms9N) and Stenotrophomonas maltophilia (Ll4); both PGPR bacteria are capable of producing siderophores, but only Ll4 have chitinase activity (Kępczyńska and Karczyński 2020 ) . Thus, the present study was aimed at (i) finding out whether the two M. truncatula PGPB identified earlier, namely Pseudomonas fluorescens (Ms9N) and Stenotrophomonas maltophilia (Ll4) are capable of directly controlling the mycelial growth of three legume soil-borne fungi: Fusarium culmorum Cul-3, F. oxysporum 857 and F. oxysporum f. sp.…”

Section: Introductionmentioning

confidence: 99%

Two Medicago truncatula growth-promoting rhizobacteria capable of limiting in vitro growth of the Fusarium soil-borne pathogens modulate defense genes expression

Karczyński

Orłowska

Kępczyńska

2023

Planta

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Main conclusion PGPRs: P. fluorescens Ms9N and S. maltophilia Ll4 inhibit in vitro growth of three legume fungal pathogens from the genus Fusarium. One or both trigger up-regulation of some genes (CHIT, GLU, PAL, MYB, WRKY) in M. truncatula roots and leaves in response to soil inoculation. Abstract Pseudomonas fluorescens (referred to as Ms9N; GenBank accession No. MF618323, not showing chitinase activity) and Stenotrophomonas maltophilia (Ll4; GenBank accession No. MF624721, showing chitinase activity), previously identified as promoting growth rhizobacteria of Medicago truncatula, were found, during an in vitro experiment, to exert an inhibitory effect on three soil-borne fungi: Fusarium culmorum Cul-3, F. oxysporum 857 and F. oxysporum f. sp. medicaginis strain CBS 179.29, responsible for serious diseases of most legumes including M. truncatula. S. maltophilia was more active than P. fluorescens in suppressing the mycelium growth of two out of three Fusarium strains. Both bacteria showed β-1,3-glucanase activity which was about 5 times higher in P. fluorescens than in S. maltophilia. Upon soil treatment with a bacterial suspension, both bacteria, but particularly S. maltophilia, brought about up-regulation of plant genes encoding chitinases (MtCHITII, MtCHITIV, MtCHITV), glucanases (MtGLU) and phenylalanine ammonia lyases (MtPAL2, MtPAL4, MtPAL5). Moreover, the bacteria up-regulate some genes from the MYB (MtMYB74, MtMYB102) and WRKY (MtWRKY6, MtWRKY29, MtWRKY53, MtWRKY70) families which encode TFs in M. truncatula roots and leaves playing multiple roles in plants, including a defense response. The effect depended on the bacterium species and the plant organ. This study provides novel information about effects of two M. truncatula growth-promoting rhizobacteria strains and suggests that both have a potential to be candidates for PGPR inoculant products on account of their ability to inhibit in vitro growth of Fusarium directly and indirectly by up-regulation of some defense priming markers such as CHIT, GLU and PAL genes in plants. This is also the first study of the expression of some MYB and WRKY genes in roots and leaves of M. truncatula upon soil treatment with two PGPR suspensions.

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Medicago truncatula root developmental changes by growth-promoting microbes isolated from Fabaceae, growing on organic farms, involve cell cycle changes and WOX5 gene expression

Cited by 7 publications

References 68 publications

Strain-Specific Biostimulant Effects of Chlorella and Chlamydomonas Green Microalgae on Medicago truncatula

Strain-Specific Biostimulant Effects of Chlorella and Chlamydomonas Green Microalgae on Medicago truncatula

Lupin, a Unique Legume That Is Nodulated by Multiple Microsymbionts: The Role of Horizontal Gene Transfer

Two Medicago truncatula growth-promoting rhizobacteria capable of limiting in vitro growth of the Fusarium soil-borne pathogens modulate defense genes expression

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