Mediators of Acute Inflammation in Leucocyte Lysosomes

Janoff, Aaron; Schaefer, Sonja

doi:10.1038/213144a0

Cited by 33 publications

(9 citation statements)

References 14 publications

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“…Arginine or lysine-rich histones and poly-1-lysine have been shown to release histamine from mast cells, (15) and rat eosinophil peroxidase has been implicated as a mediator of histamine release (4). Although it is possible that some of these substances are active in our system, identification of the histamine-releasing agents in human lymphocytes and granulocytes must await purification of the active principles involved.…”

Section: Discussionmentioning

confidence: 99%

Mediators of histamine release from human platelets, lymphocytes, and granulocytes

Kelly¹,

Martin²,

White³

1971

J. Clin. Invest.

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A B S T R AC T Lysates of mixed human leukocyte suspensions released histamine from intact human leukocytes in vitro. Microgram quantities of leukocyte lysate protein released up to 90% of the total available histamine. The mixed leukocyte lysates were separated by differential centrifugation into nuclear (800 g pellet), lysosomal (25,000 g pellet), and postlysosomal supernatant (25,000 g supernatant) fractions. The degree of separation of the lysosomal from the other two fractions was assessed by measuring the relative activities of four lysosomal enzymes. The average distribution of enzyme activity was 11 ±2% (mean ±1 SD), 72 ±10%, and 17 +8% for the nuclear, lysosomal, and supernatant fractions respectively. The histamine-releasing activity was equally distributed between the lysosomal and supernatant fractions, each of which had 5-fold greater activity than the nuclear fraction.Purified suspensions of platelets, lymphocytes, and granulocytes were prepared, and the lysates of these suspensions all had histamine-releasing activity. Centrifugation at 100,000 g for 18 hr sedimented the histaminereleasing activity from all three types of lysate. After 20% ethanol fractionation for the preparation of cationic protein, only the activity from the platelet lysates was found in the 20% ethanol insoluble fraction.These mediators of histamine release from human platelets, lymphocytes, and granulocytes may play a role in the development of the vasodilation and increased vascular permeability which characterize the acute inflamniatory response. INTRODUCTIONThe acute inflammatory response is characterized by certain vascular phenomena including vasodilation and increased vascular permeability. Various naturally oc-A preliminary report of this work appeared in abstract form in 1969. Clin. Res. 17: 541.

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Section: Discussionmentioning

confidence: 99%

Mediators of histamine release from human platelets, lymphocytes, and granulocytes

Kelly¹,

Martin²,

White³

1971

J. Clin. Invest.

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“…A similar release may have oc curred from the cells trapped during the injection of ALG. Platelets, neu trophils as well as lymphocytes contain a cationic lysosomal protein which induces histamine release from mast cells [15,21,31]. The capaci ty of that histamine-releasing mechanism has been demonstrated by injec tion of lysates of platelets, neutrophils or lymphocytes in dogs.…”

Section: Discussionmentioning

confidence: 99%

Role of Platelets and Lymphocytes for the Hemodynamic Effects of Rapidly Injected Antilymphocyte Globulin

Henricsson¹,

Bergentz²

1978

Eur Surg Res

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Xenogenic antilymphocyte globulin (ALG) given as a rapid intravenous injection into dogs caused a significant decrease in arterial blood pressure and oxygenation as well as an increase in portal pressure. At the same time, there was an almost complete elimination of lymphocytes and an extensive but almost completely reversible thrombocytopenia. Lymphocytopenia induced by a previous injection of ALG was found to eliminate completely the circulatory response to a second injection of ALG in spite of the fact that an extensive thrombocytopenia was induced. Extensive thrombocytopenia induced by injection of an antiplatelet globulin was also found to prevent completely the hemodynamic effects of ALG although as many lymphocytes were eliminated as in the intact dogs. Thus both platelets and lymphocytes are necessary to trigger the circulatory response induced by ALG.

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“…It can be suggested that few molecules of degranulators induce release from eosinophils to any great extent of mast cell lytic polypeptides whose cytotoxic effect, as demonstrated by Janoff and Schaefer [1967], almost equalizes in terms of molar concentration the one of 48/80.…”

Section: Discussionmentioning

confidence: 99%

Isolation of Mast Cell Degranulators from the Celomatic Fluid of <i>Parascaris equorum</i>

Tolone¹,

Bonasera²,

Brai³

et al. 1974

Pathobiology

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Two mast cell degranulating factors, which closely resemble proteins in their chemical-physical behavior, were isolated from the celomatic fluid of Parascaris equorum und highly purified by combinations of salting out, ion-exchange chromatography and isoelectrofocusing. Both factors proved to be positively charged molecules of molecular weight close to 3-4 × 10⁴ which, in terms of molar concentration, were as efficient as compound 48/80 in selective induction of histamine release.

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Mediators of Acute Inflammation in Leucocyte Lysosomes

Cited by 33 publications

References 14 publications

Mediators of histamine release from human platelets, lymphocytes, and granulocytes

Mediators of histamine release from human platelets, lymphocytes, and granulocytes

Role of Platelets and Lymphocytes for the Hemodynamic Effects of Rapidly Injected Antilymphocyte Globulin

Isolation of Mast Cell Degranulators from the Celomatic Fluid of <i>Parascaris equorum</i>

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